Publications by authors named "Muhammad Wajid Ullah"

Sustainable lignin-based materials are becoming increasingly valuable in agriculture, where climate change and nutrient deficiencies threaten crop productivity. We developed lignin-derived cryogels using waste biomass to improve soil nutrients, seed germination, water retention, and photosynthetic pigment levels. These cryogels were synthesized with gum Arabic (GA), keratin (K), and N-vinylpyrrolidone at lignin concentrations of 0.

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This study presents the ex situ development and characterization of bacterial cellulose (BC) membranes loaded with bioactive Sage and Neem extracts for enhanced antimicrobial applications. Utilizing discarded fruit waste as a cost-effective carbon source, BC production was optimized, yielding membranes with improved properties. Neem and Sage extracts, obtained via Soxhlet extraction, exhibited significant antibacterial activity against Escherichia coli and Staphylococcus aureus, with minimum inhibitory concentrations of 3.

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Article Synopsis
  • Ammonia (NH) is a toxic gas that negatively impacts the environment and the immune systems of chickens, although the exact mechanisms behind these effects are not fully understood.
  • In this study, researchers used advanced imaging techniques to observe structural damage in the bursa of Fabricius and thymus in chickens exposed to NH, including swollen mitochondria and other cellular changes.
  • The exposure also led to an enhanced immune response, activating specific inflammatory pathways and upregulating important genes related to inflammation, which may inform future treatments to support poultry health.
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Article Synopsis
  • The text highlights the growing interest in lignin, a biopolymer, due to its potential for creating eco-friendly products, with β-etherases being critical for breaking down its complex structure.
  • It provides an overview of recent advancements in β-etherase research, including their substrate selectivity, oxidative activity, and engineering methods, which enhance our understanding of their applications.
  • The review also addresses the challenges and promising future of β-etherases in lignin valorization for sustainable and economically viable biorefinery processes.
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Recent advancements in in vitro transcribed mRNA (IVT-mRNA) vaccine manufacturing have attracted considerable interest as advanced methods for combating viral infections. The respiratory mucosa is a primary target for pathogen attack, but traditional intramuscular vaccines are not effective in generating protective ion mucosal surfaces. Mucosal immunization can induce both systemic and mucosal immunity by effectively eliminating microorganisms before their growth and development.

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Nanocellulose (NC) is a promising biopolymer for various biomedical applications owing to its biocompatibility and low toxicity. However, it faces challenges in tissue engineering (TE) applications due to the inconsistency of the microenvironment within the NC-based scaffolds with target tissues, including anisotropy microstructure and biomechanics. To address this challenge, a facile swelling-induced nanofiber alignment and a novel in situ biomineralization reinforcement strategies were developed for the preparation of NC-based scaffolds with tunable anisotropic structure and mechanical strength for guiding the differentiation of bone marrow-derived mesenchymal stem cells for potential TE application.

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Soybean research has gained immense attention due to its extensive use in food, feedstock, and various industrial applications, such as the production of lubricants and engine oils. In oil crops, the process of seed development and storage substances accumulation is intricate and regulated by multiple transcription factors (TFs). In this study, FUSCA3 (GmFUS3) was characterized for its roles in plant development, lipid metabolism, and stress regulation.

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Montmorillonite clay and agar are naturally occurring materials of significant importance in designing biocompatible materials tailored for applications in biotechnology and medicine. The introduction of magnetic properties has the potential to significantly boost their characteristics and expand their applications. In this study, we have successfully synthesized highly intercalated magnetic composites, incorporating magnetic iron oxide nanoparticles (MNPs), montmorillonite clay (MMT), and agar (AG), through a thermo-physicomechanical method.

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Cellulose, the most abundant polymer on Earth, has been widely utilized in its nanoform due to its excellent properties, finding applications across various scientific fields. As the demand for nanocellulose continues to rise and its ease of use becomes apparent, there has been a significant increase in research publications centered on this biomaterial. Nanocellulose, in its different forms, has shown tremendous promise as a tissue engineered scaffold for regeneration and repair.

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Industrialization and population growth are leading to the production of significant amounts of sewage containing hazardous xenobiotic compounds. These compounds pose a threat to human and animal health, as well as the overall ecosystem. To combat this issue, chemical, physical, and biological techniques have been used to remove these contaminants from water bodies affected by human activity.

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Nylon-6,6 microplastics (NMPs) in aquatic systems have emerged as potential contaminants to the global environment and have garnered immense consideration over the years. Unfortunately, there is currently no efficient method available to eliminate NMPs from sewage. This study aims to address this issue by isolating Brucella intermedia ZL-06, a bacterium capable of producing a bacterial polysaccharide-based flocculant (PBF).

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Due to the limited resources and environmental problems associated with fossil fuels, there is a growing interest in utilizing renewable resources for the production of biofuels through microbial fermentation. Isobutanol is a promising biofuel that could potentially replace gasoline. However, its production efficiency is currently limited by the use of naturally isolated microorganisms.

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Weed communities influence the dynamics of ecosystems, particularly in disturbed environments where anthropogenic activities often result in higher pollution. Understanding the dynamics existing between native weed communities and invasive species in disturbed environments is crucial for effective management and normal ecosystem functioning. Recognising the potential resistance of native weed communities to invasion in disturbed environments can help identify suitable native plants for restoration operations.

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Hexabromocyclododecane (HBCD) is a widely used brominated flame retardant; however, it is a persistent organic pollutant as well as affects the human thyroid hormones and causes cancer. However, the degradation of HBCD has received little attention from researchers. Due to its bioaccumulative and hazardous properties, an appropriate strategy for its remediation is required.

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Benzene, a potent carcinogen, is known to cause acute myeloid leukaemia. While chemotherapy is commonly used for cancer treatment, its side effects have prompted scientists to explore natural products that can mitigate the haematotoxic effects induced by chemicals. One area of interest is nano-theragnostics, which aims to enhance the therapeutic potential of natural products.

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Transcatheter arterial embolization, a minimally invasive treatment to deliberately occlude the blood vessels, has become a safe and effective procedure for the management of vascular diseases and benign/malignant tumors. Particularly, hydrogel-based embolic agents have garnered much attention because of their potential to address some of the limitations of clinically used embolic agents and can be rationally designed to impart more favorable characteristics or functions. In this review, the recent progress toward the development of polymer-based hydrogels for effective endovascular embolization, including the in situ gelling hydrogels mediated by physically or chemically crosslinking, imageable hydrogels for intraprocedural and postprocedural feedback, use of hydrogels as the drug depot for local delivery of therapeutic drugs, hemostatic hydrogels inducing extrinsic or intrinsic coagulation of blood, stimuli-responsive shape memory hydrogels as the smart embolization devices, and hydrogels incorporating external-stimuli functional materials for multidisciplinary therapy, is systemically summarized.

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The anisotropic features play indispensable roles in regulating various life activities in different organisms. Increasing efforts have been made to learn and mimic various tissues' intrinsic anisotropic structure or functionality for broad applications in different areas, especially in biomedicine and pharmacy. This paper discusses the strategies for fabricating biomaterials using biopolymers for biomedical applications with the case study analysis.

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Bacteriophages are the most abundant biological entity in the world and hold a tremendous amount of unexplored genetic information. Since their discovery, phages have drawn a great deal of attention from researchers despite their small size. The development of advanced strategies to modify their genomes and produce engineered phages with desired traits has opened new avenues for their applications.

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This study was aimed to develop low-cost bacterial cellulose (BC)-based antibacterial composite with pomegranate (Punica granatum L.) peel extract (PGPE) for potential biomedical applications. BC was cost-effectively produced by utilizing food wastes, and PGPE was ex situ impregnated into its hydrogel.

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A major challenge to large-scale production and utilization of bacterial cellulose (BC) for various applications is its low yield and productivity by bacterial cells and the high cost of feedstock. A supplementation of the classical expensive Hestrin and Schramm (HS) medium with 1 % polyethylene terephthalate ammonia hydrolysate (PETAH) resulted in 215 % high yield. Although the physicochemical properties of BC were not significantly influenced, the BC produced in 1 % PETAH-supplemented HS medium showed a higher surface area, which showed 1.

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Bacterial cellulose (BC) is currently one of the most popular environmentally friendly materials with unique structural and physicochemical properties for obtaining various functional materials for a wide range of applications. In this regard, the literature reporting on bacterial nanocellulose has increased exponentially in the past decade. Currently, extensive investigations aim at promoting the manufacturing of BC-based nanocomposites with other components such as nanoparticles, polymers, and biomolecules, and that will enable to develop of a wide range of materials with advanced and novel functionalities.

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Deterioration in the environmental ecosystems through the depletion of nonrenewable resources and the burden of deleterious contaminants is considered a global concern. To this end, great interest has been shown in the use of renewable and environmentally-friendly reactive materials dually to promote environmental sustainability and cope with harmful contaminants. Among the different available options, the use of nanocellulose (NC) as an environmentally benign and renewable natural nanomaterial is an attractive candidate for environmental remediation owing to its miraculous physicochemical characteristics.

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