Fabrication and evaluation of chondroitin sulfate based hydrogels loaded with chitosan nanoparticles for oral delivery of vildagliptin.

Vildagliptin is a drug of choice in type II diabetes mellitus that suffers from limitations like short half-life with reduced bioavailability. To improve the therapeutic performance of vildagliptin, this study aimed to synthesize chitosan nanoparticles (NPs) loaded hydrogel by using biological polysaccharides like sodium alginate (SA) and chondroitin sulfate (CS). The NPs were prepared by ionic gelation method and various characterization tests like surface morphology, size and zeta potential, entrapment efficiency, and in-vitro drug release studies were performed.

3D MFA: An automated 3D Multi-Feature Attention based approach for spine segmentation using a multi-stage network pruning.

Spine segmentation poses significant challenges due to the complex anatomical structure of the spine and the variability in imaging modalities, which often results in unclear boundaries and overlaps with surrounding tissues. In this research, a novel 3D Multi-Feature Attention (MFA) model is proposed for spine segmentation. The standard MobileNetv3 is modified by adding the RCBAM (Reverse Convolution Block Attention Module) module, and FPP (Feature Pyramid Pooling) for feature enhancement.

Renewable energy resource management using an integrated robust decision making model under entropy and similarity measures of fuzzy hypersoft set.

The demand for renewable energy has significantly increased over the last decade with increased attention to the preservation of the environment and sustainable, optimal resource management. As traditional sources of energy production are depleting at an alarming rate and causing long-lasting environmental damage, it is essential to explore green and cost-effective methodologies for meeting energy demand. With each country having different geographical, political, social, and natural factors, the problem arises of which renewable energy should be utilized for optimal resource management.

Ring opening of epoxides: a facile approach towards the synthesis of polyketides and related stereoenriched natural products: a review.

Epoxides are significant heterocycles in the structural makeup of a variety of natural products. Their ring-opening reactions have emerged as a versatile and efficient strategies for synthesizing a variety of functionalized molecules. Such reactions have been extensively applied towards the preparation of complex naturally occurring products.

Spatial microenvironment enhanced photocatalytic reduction of uranyl ions under solar light irradiation.

Photocatalytic reduction of uranyl ions (UO) is an environmentally friendly, energy efficient, and highly effective method for uranium-containing wastewater treatment and uranium recovery. Herein, a novel photocatalytic material CH-8 @NNFO-4 with abundant oxygen vacancies was synthesize by growing Ca(OH) on the surface of Fe doped NaNbO in situ. The Ca(OH) synergizes with the oxygen vacancies, creating a microenvironment that narrows the bandgap and extends the light response range.

Distribution of HPV Types in Cervical Cancer in Pakistan: Implications for Screening and Vaccination Programs.

Background: HPV plays a key role in the development of cervical cancer. This study aims to investigate the prevalence of HPV genotypes in patients with Squamous cell carcinoma (SSC) and Adenocarcinoma (ADC) at NORI cancer Hospital Pakistan, with the aim of improving screening and prevention strategies.

Method: Cervical scrapings were collected from 129 diagnosed cervical cancer patients.

Methodological optimization for efficient degradation of Acid Violet 49 using advanced oxidation processes and varied photocatalyst combinations.

This study investigates the photodegradation of Acid Violet 49 in an aqueous medium under UV, UV/HO and combined with photo catalyst (UV/HO/TiO, UV/HO/ZnO, and UV/HO/SnO). The impact of all operational parameters including catalytic dosage, peroxidation (HO), pH and dye concentration were evaluated. The degradation efficiency of AV49 was enhanced up to 96% with the alternative photocatalyst UV/HO/ZnO under the circumstances of 0.

Three-step surface design of spore-displayed keratinase improved acid tolerance and feather degradation.

The global chicken business has grown rapidly, producing millions of tons of feather waste annually. Keratinase is a special enzyme that catalyzes the degradation of keratin and can be applied to the feed industry. In this study, we initially set the tone for the acid-resistant mutation of spore surface-display keratinase cotG-KERQ7 by replacing base-catalytic residues in the active center.

Bevacizumab for the treatment of metastatic colorectal cancer complicated by abdominal aortic aneurysm and mural thrombus: a case report.

Bevacizumab, a humanized monoclonal antibody targeting vascular endothelial growth factor (VEGF), was the first anti-angiogenic agent incorporated into metastatic colorectal cancer treatment strategies and demonstrated broad-spectrum anti-tumor efficacy. Commonly reported adverse events include hypertension, proteinuria, gastrointestinal perforation, bleeding, and thromboembolism. However, there are only a few reports on abdominal aortic aneurysms (AAA) as a complication of bevacizumab therapy.

Dynamics simulations of hypoxia inducible factor-1 regulatory network in cancer using formal verification techniques.

Hypoxia-inducible factor-1 (HIF-1) regulates cell growth, protein translation, metabolic pathways and therefore, has been advocated as a promising biological target for the therapeutic interventions against cancer. In general, hyperactivation of HIF-1 in cancer has been associated with increases in the expression of glucose transporter type-1 (GLUT-1) thus, enhancing glucose consumption and hyperactivating metabolic pathways. The collective behavior of GLUT-1 along with previously known key players AKT, OGT, and VEGF is not fully characterized and lacks clarity of how glucose uptake through this pathway (HIF-1) probes the cancer progression.

Prevalence and associated risk factors of Brucella abortus and Brucella melitensis in humans and cattle populations: A comprehensive study.

A zoonotic disease unique to South Asia, brucellosis causes major public health and financial problems, notably in Pakistan. This research aimed to ascertain the molecular frequency of Brucella abortus and Brucella melitensis in humans and cattle and to pinpoint related risk factors in the Southern Khyber Pakhtunkhwa, Pakistan, districts. A total of 800 blood samples, 600 from cattle and 200 from humans, were gathered and examined using traditional PCR targeting the IS711 locus.

Predictive analytics of complex healthcare systems using deep learning based disease diagnosis model.

Cancer is a life-threatening disease resulting from a genetic disorder and a range of metabolic anomalies. In particular, lung and colon cancer (LCC) are among the major causes of death and disease in humans. The histopathological diagnoses are critical in detecting this kind of cancer.

High-throughput screening and identification of lignin peroxidase based on spore surface display of Bacillus subtilis.

Background: Lignin peroxidase is closely related to agriculture and food as it improves the quality of feedstuffs, facilitates the degradation of lignin in agricultural wastes, and degrades azo dyes that have similar complex structures to lignin. However, the current status of homologous or heterologous expression of lignin peroxidase is unsatisfactory and needs to be modified with the help of immobilization and directed evolution to maximize its potential. Directed evolution technology is an effective strategy for designing and improving enzyme characteristics, and Bacillus subtilis spore surface display technology is an efficient method for preparing immobilized enzymes.

Pharmacogenomic Study of Selected Genes Affecting Amlodipine Blood Pressure Response in Patients with Hypertension.

Introduction: Despite the availability of various antihypertensive medications, the response to these medications varies among individuals. Understanding how individual genetic variations affect drugs treatment outcomes is a key area of focus in precision medicine. This study investigated the correlation between single nucleotide polymorphisms (SNPs) in selected genes (CACNA1C, CACNA1D, ABCB1, ACE, ADBR2, and NOS1AP) and the blood pressure (BP) control by amlodipine.

Exploring the factors affecting career progression in informal faculty mentoring sessions within mentor and mentee relationships: a qualitative study.

Background: Mentoring plays a pivotal role in mentees' professional advancement. However, the factors that affect career progression in informal mentoring relationships, especially with respect to faculty members, have not been extensively explored. This study aimed to explore the factors that affect career progression in informal faculty mentorings within mentor and mentee relationships.

Molecular prevalence, phylogenetic analysis, and PCR-based detection of Brucella melitensis in humans and cattle in Southern Khyber Pakhtunkhwa, Pakistan.

Brucellosis is a bacterial disease due to Brucella melitensis, considered a zoonotic agent affecting humans and animals, especially in areas with high disease occurrence, south Khyber Pakhtunkhwa, Pakistan. This work was designed to evaluate the molecular rate of B. melitensis in humans and cattle species and also to perform a phylogenetic analysis between both species.

Feeding and Growth Response of Fall Armyworm towards Different Host Plants.

The fall armyworm, , is a major migratory polyphagous insect pest of various crops. The essential nutrient and mineral profile of the host plants determines the feeding fitness of herbivorous insects. As a result, the growth and development of insects is affected.

AbAMPdb: a database of Acinetobacter baumannii specific antimicrobial peptides.

Acinetobacter baumannii has emerged as a prominent nosocomial pathogen, exhibiting a progressive rise in resistance to therapeutic interventions. This rise in resistance calls for alternative strategies. Here, we propose an alternative yet specialized resource on antimicrobial peptides (AMPs) against A.

Optimization study and application of box-behnken model for probing eggshell supported transition metals based catalysts to synthesize hydrazone & dihydropyrimidinones.

Solid supported catalysts have several synthetic applications. Herein, finely ground eggshells were used as a solid support for the preparation of transition metal (Ni, Zn, Cu, Sn and Co) based catalysts to synthesize 2,4-dinitrophenylhydrazone (3) and dihydropyrimidinones (7 and 8). The effect of catalyst load, time and temperature on product yield was studied.