Enhanced Stability and Reusability of Subtilisin Carlsberg Through Immobilization on Magnetic Nanoparticles.

Introduction: Immobilizing enzymes on solid supports such as magnetic nanoparticles offers multi-dimensional advantages, including enhanced conformational, structural, and thermal stability for long-term storage and reusability.

Methodology: The gene encoding subtilisin Carlsberg was isolated from proteolytic , a bacterium derived from salt mines. The nucleotide sequence encoding pro-peptide and mature protein were cloned into pET22(a)+ vector and expressed in .