Detection of Residual Antibiotics and Their Differential Distribution in Broiler Chicken Tissues Using Enzyme-Linked Immunosorbent Assay.

The aim of this study was to estimate the residue levels of five commonly used antibiotics in poultry tissue samples using enzyme linked immunosorbent assay (ELISA). A total of 200 samples that comprised breast and liver (100 each) were collected from five poultry farms randomly selected from Muscat regions. The samples were analyzed for enrofloxacin (ENR), gentamicin (GEN), oxytetracycline (OTC), sulfamethazine (SMZ), and tylosin (TYL) residue concentrations.

Concurrent vaccination of goats with foot and mouth disease (FMD) and peste des petits ruminants (PPR) booster vaccines.

Foot and mouth disease (FMD) remains subclinical and self-limiting in small ruminants, but risk of spread of infection to susceptible cohorts is of great epidemiological significance; therefore, small ruminants must be included in vaccination campaigns in FMD endemic regions. Three groups of goats already immunized against peste des petits ruminants (PPR) were vaccinated with FMD and PPR vaccines alone or concurrently. The specific antibody response against three FMD virus strains and PPR virus were evaluated by competitive enzyme-linked immunosorbent assay (cELISA).

Seroprevalence of Q Fever (Coxiellosis) in Small Ruminants of Two Districts in Punjab, Pakistan.

Coxiellosis caused by Coxiella burnetii is a cosmopolitan zoonosis, which causes significant losses through abortions and stillbirths in small ruminants. A cross-sectional seroprevalence study was conducted in two major sheep and goat farming districts of Punjab (Layyah and Muzaffargarh), Pakistan. In total, 542 small ruminants (271 sheep and goats each) of both sexes (60 males and 482 females) of different age groups from 104 flocks (52 flocks of either species) were randomly selected for the collection of sera and related epidemiological information.

Seroprevalence of Babesia caballi and Theileria equi in five draught equine populated metropolises of Punjab, Pakistan.

Equine piroplasmosis (EP) caused by intraerythrocytic parasites (Theileria equi and Babesia caballi) is an emerging equine disease of world-wide distribution. In Pakistan, the prevalence and incidence of EP are unknown. In order to obtain the first insights into the prevalence of the disease, a total of 430 equids, including 33 mules, 65 horses and 332 donkeys, aging from ≤ 5 to ≥ 10 years of either sex, from five metropolises of Punjab, Pakistan, were serologically tested for the presence of antibodies directed against B.

Evaluation of a 3% surf solution (surf field mastitis test) for the diagnosis of subclinical bovine and bubaline mastitis.

Purpose: To evaluate a 3% solution of household detergent viz., Surf Excel (Surf field mastitis test, SFMT) vis-à-vis California mastitis test (CMT), Whiteside test (WST), somatic cell counts (SCC; cut off limit = 5 x 10(5) cells per millilitre) and bacteriological cultures for the detection of subclinical mastitis in quarter foremilk samples (n=800) of dairy cows and buffaloes.

Methods: Culture and SCC were used as gold standards.

Comparative evaluation of Rose Bengal plate agglutination test, mallein test, and some conventional serological tests for diagnosis of equine glanders.

The Rose Bengal plate agglutination test (RBT) was evaluated for the diagnosis of equine glanders, and its diagnostic efficiency was compared with that of mallein and other serological tests, including indirect hemagglutination test (IHAT), complement fixation test (CFT), and modified counter immunoelectrophoresis test (mCIET). Sera from 70 naturally infected culture-positive, 96 potentially exposed cohorts, and 110 healthy equines were tested. All tests but mCIET showed 100% specificity when testing the sera from glanders-negative equines.