Publications by authors named "Muhammad K R Khan"

Heat stress poses a significant environmental challenge that profoundly impacts wheat productivity. It disrupts vital physiological processes such as photosynthesis, by impeding the functionality of the photosynthetic apparatus and compromising plasma membrane stability, thereby detrimentally affecting grain development in wheat. The scarcity of identified marker trait associations pertinent to thermotolerance presents a formidable obstacle in the development of marker-assisted selection strategies against heat stress.

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Human activities and climate change have resulted in frequent and intense weather fluctuations, leading to diverse abiotic stresses on crops which hampers greatly their metabolic activities. Heat stress, a prevalent abiotic factor, significantly influences cotton plant biological activities resulting in reducing yield and production. We must deepen our understanding of how plants respond to heat stress across various dimensions, encompassing genes, RNAs, proteins, metabolites for effective cotton breeding.

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Background: Aldehyde dehydrogenases (ALDHs) are a family of enzymes that catalyze the oxidation of aldehyde molecules into the corresponding carboxylic acid, regulate the balance of aldehydes and protect plants from the poisoning caused by excessive accumulation of aldehydes; however, this gene family has rarely been studied in cotton.

Results: In the present study, genome-wide identification was performed, and a total of 114 ALDH family members were found in three cotton species, Gossypium hirsutum, Gossypium arboreum and Gossypium raimondii. The ALDH genes were divided into six subgroups by evolutionary analysis.

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Cotton ( L.) is a significant fiber crop. Being a major contributor to the textile industry requires continuous care and attention.

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Analysis of fiber quality lncRNAs and their target genes from a pair of Gossypium mustelinum near-isogenic lines provide new prospects for improving the fiber quality of Upland cotton. Long noncoding RNAs (lncRNAs) are an important part of genome transcription and play roles in a wide range of biological processes in plants. In this research, a pair of near-isogenic cotton lines, namely, a Gossypium mustelinum introgression line (IL9) with outstanding fiber quality and its recurrent Upland cotton parent (PD94042), were used as the experimental materials.

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Plant Carbonic anhydrases (Cas) have been shown to be stress-responsive enzymes that may play a role in adapting to adverse conditions. Cotton is a significant economic crop in China, with upland cotton (Gossypium hirsutum) being the most widely cultivated species. We conducted genome-wide identification of the βCA gene in six cotton species and preliminary analysis of the βCA gene in upland cotton.

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Background: As the world's leading fiber crop and a major oil-producing crop, cotton fiber yield and fiber quality are affected by environmental stresses, especially heat, drought and salinity. The LAZ1 (Lazarus 1) family genes are responsive to abscisic acid, drought, and salt treatments. Currently, mining and functional analyses of LAZ1 family genes in cotton have not been reported.

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To investigate the toxic effects of lead (Pb) on key metabolic activities essential for proper germination and seedling growth of maize seeds, experiments were carried out with different levels of Pb (0 to 120 mg of Pb L as PbCl) applied through growth medium to two maize hybrids H-3310S and H-6724. The research findings indicated that growth and metabolic activities were adversely affected by increased Pb contamination in growth medium; however, a slow increase in these parameters was recorded with increasing time from 0 to 120 h. Protease activity decreased with an increase in the level of Pb contamination but increased with time; consequently, a reduction in seed proteins and an increase in total free amino acids were observed with time.

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Cottonseed is an invaluable resource, providing protein, oil, and abundant minerals that significantly contribute to the well-being and nutritional needs of both humans and livestock. However, cottonseed also contains a toxic substance called gossypol, a secondary metabolite in Gossypium species that plays an important role in cotton plant development and self-protection. Herein, genome-wide analysis and characterization of the terpene synthase (TPS) gene family identified 304 TPS genes in Gossypium.

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Article Synopsis
  • Climate change is increasing salt stress, negatively impacting the quality and yield of cotton crops, especially during seedling and germination phases.
  • High salt levels lead to issues like delayed flowering, fewer fruits, and poor fiber quality, ultimately harming seed cotton outputs.
  • The review discusses the mechanisms of salt tolerance, the benefits of modern breeding methods like marker-assisted selection, and explores new strategies for developing salt-tolerant cotton varieties.
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Background: Geranylgeranyl pyrophosphate synthase (GGPS) is a structural enzyme of the terpene biosynthesis pathway that is involved in regulating plant photosynthesis, growth and development, but this gene family has not been systematically studied in cotton.

Results: In the current research, genome-wide identification was performed, and a total of 75 GGPS family members were found in four cotton species, Gossypium hirsutum, Gossypium barbadense, Gossypium arboreum and Gossypium raimondii. The GGPS genes were divided into three subgroups by evolutionary analysis.

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Uncovering the underlying mechanism of salt tolerance is important to breed cotton varieties with improved salt tolerance. In this study, transcriptome and proteome sequencing were performed on upland cotton ( L.) variety under salt stress, and integrated analysis was carried out to exploit salt-tolerance genes in cotton.

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Salinity stress is one of the primary threats to agricultural crops resulting in impaired crop growth and development. Although cotton is considered as reasonably salt tolerant, it is sensitive to salt stress at some critical stages like germination, flowering, boll formation, resulting in reduced biomass and fiber production. The mechanism of partial ion exclusion (exclusion of Na and/or Cl) in cotton appears to be responsible for the pattern of uptake and accumulation of harmful ions (Na and Cl) in tissues of plants exposed to saline conditions.

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Article Synopsis
  • Upland cotton is significantly impacted by salt stress during its growth, and the GUB_WAK_bind gene plays a critical role in the plant's stress response.
  • Researchers identified and analyzed 22 GUB_WAK_bind gene members in Gossypium hirsutum using bioinformatics, revealing their gene structure and positioning, as well as their expression under salt stress.
  • The study found that these genes are evolutionarily divided into three subgroups and are mostly induced by NaCl stress, contributing valuable insights into the salt tolerance mechanisms of cotton.
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  • CLASPs are proteins that interact with microtubules, crucial for plant growth, yet little is known about their role in upland cotton specifically.
  • This study conducted a genome analysis of the CLASP_N gene family in upland cotton, utilizing bioinformatics tools and real-time PCR to investigate their characteristics and expression patterns.
  • The research identified 16 CLASP_N genes, noted their chromosomal locations, and revealed that they are highly expressed during later stages of fiber development, paving the way for further investigation of their molecular functions in cotton.
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  • The study focuses on plant cytochrome P450 (P450) genes, which are important for various biological processes, including the synthesis of plant hormones and defensive compounds, particularly in salt-tolerant upland cotton "Tongyan No. 1."
  • Using bioinformatics and qRT-PCR, researchers analyzed the P450 genes' expression patterns and their structural variations, identifying 156 P450 genes with diverse characteristics like gene length and intron/exon numbers.
  • The findings suggest a link between P450 gene expression and salt tolerance in cotton seedlings, providing insights for future research on the P450 gene family and its evolutionary relationships.
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Cotton is an important fiber and cash crop. Extreme water scarceness affects the growth, quality, and productivity of cotton. Water shortage has threatened the future scenario for cotton growers, so it is imperative to devise a solution to this problem.

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Cotton is a primary agriculture product important for fiber use in textiles and the second major oil seed crop. Cotton is considered as moderately tolerant to salt stress with salinity threshold of 7.7 dS/m at seedling stage.

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This study evaluated the genetic diversity and population structures in a novel cotton germplasm collection comprising 132 diploids, including and allotetraploid cotton accessions, including , , , , and , from Santa Cruz, Isabella, San Cristobal, Hawaiian, Dominican Republic, and Wake Atoll islands. A total of 111 expressed sequence tag (EST) and genomic simple sequence repeat (gSSR) markers produced 382 polymorphic loci with an average of 3.44 polymorphic alleles per SSR marker.

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A high density genetic map was constructed using F2 population derived from an interspecific cross of G. hirsutum × G. tomentosum.

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