An Efficient Method for Isolating and Purifying Nuclei from Mice Brain for Single-Molecule Imaging Using High-Speed Atomic Force Microscopy.

Nuclear pore complexes (NPCs) on the nuclear membrane surface have a crucial function in controlling the movement of small molecules and macromolecules between the cell nucleus and cytoplasm through their intricate core channel resembling a spiderweb with several layers. Currently, there are few methods available to accurately measure the dynamics of nuclear pores on the nuclear membranes at the nanoscale. The limitation of traditional optical imaging is due to diffraction, which prevents achieving the required resolution for observing a diverse array of organelles and proteins within cells.

The rise of circulatory endothelin (ET)-1 and endothelin receptors (ET, ET) expression in kidney of obese wistar rat.

Background: Endothelin (ET)-1, a circulatory protein, and its receptors (ET and ET) in various organs were reported to play a pivotal role in many diseases, including obesity. However, the changes of ET and ET expression in ventricle and kidney in obesity was less reported. The study is designed to observe the level of circulatory ET-1 and expression of ET/ET in ventricle and kidney of obese, as compared to non-obese, rats.