Comparative analysis of ACE2 mutations and their impact on SARS-CoV-2 susceptibility within diverse ethnic groups.

The COVID-19 pandemic, caused by the SARS-CoV-2 virus, has significantly impacted on global public health and economies. Emerging from China, the virus generated diverse variants with varying infectivity and drug resistance profiles. SARS-COV-2 requires an ACE-2 receptor for its adhesion, and and genetic analyses have identified multiple single-nucleotide variations in ACE2 across different human populations.

Appraisal of CRISPR Technology as an Innovative Screening to Therapeutic Toolkit for Genetic Disorders.

The high frequency of genetic diseases compels the development of refined diagnostic and therapeutic systems. CRISPR is a precise genome editing tool that offers detection of genetic mutation with high sensitivity, specificity and flexibility for point-of-care testing in low resource environment. Advancements in CRISPR ushered new hope for the detection of genetic diseases.

Participatory epidemiological approaches for risk assessment of bed bug (Insecta: Hemiptera: Cimicidae) infestation in public hospitals in the city of Faisalabad, Punjab, Pakistan.

This study was to determine risk assessment associated with bed bugs (Insecta: Hemiptera: Cimicidae) using participatory epidemiological tools in the selected public hospitals of district Faisalabad, Punjab, Pakistan. Bed bugs were collected and morphologically identified through a standard taxonomic key. A descriptive cross-sectional survey of patients using a pre-designed questionnaire containing open-ended and closed-ended questions was conducted for six months between January 2023 and June 2023.

Carvone-loaded chitosan nanoparticles alleviate joint destruction by downregulating the expression of pro, inflammatory cytokines and MMP-13 in adjuvant-induced rat model.

Rheumatoid arthritis is an autoimmune illness causing deformity, edema, and joint tenderness. Its long-term treatment burdens the healthcare system and leads to toxicity, and thus, finding safe, effective, and affordable therapies is essential. The current study aimed to exhibit the anti-arthritic activity of Carvone-loaded chitosan nanoparticles to treat Freund's complete adjuvant (FCA) arthritis in rats.

Characterizing the bacteriophage PKp-V1 as a potential treatment for ESBL-producing hypervirulent K1 ST258 isolated from veterinary specimens.

Background And Aim: The dearth of new antibiotics necessitates alternative approaches for managing infections caused by resistant superbugs. This study aimed to evaluate the lytic potential of the purified bacteriophage PKp-V1 against extended-spectrum β-lactamase (ESBL) harboring hypervirulent (hvKp)-K1 recovered from veterinary specimens.

Materials And Methods: A total of 50 samples were collected from various veterinary specimens to isolate , followed by antimicrobial susceptibility testing and molecular detection of various virulence and ESBL genes.

Dietary Chlorella vulgaris mitigates aflatoxin B1 toxicity in broiler chicken: Toxicopathological, hematobiochemical and immunological perspectives.

Mycotoxins are the chemical substances, produced as the secondary metabolites of some toxigenic species of fungi which cause critical health issues in humans, birds and different animal species while Chlorella vulgaris (CV) is a unicellular microalga which contains plenty of important nutritional ingredients. This study was planned to evaluate the toxicopathological, hematobiochemical and immune changes incurred by dietary supplementation of aflatoxin B1 (AFB1) and their mitigation through CV in broilers. For this study to be conducted, 180 broiler birds of one day old were uniformly distributed into six (06) groups and administered various combinations of AFB1 (200 μg/kg) or CV (0.

Ethanolic extract of Euphorbia royleana Boiss. reduces metastasis of breast cancer cells and inhibits tumor progression in vivo.

Metastasis is the most devastating attribute of breast cancer (BC) that leads to high mortality. It is a complex process of tumor cell migration, invasion, and angiogenesis. In this study, we evaluated the effect of ERA on BC metastasis and BC progression in vivo.

Optical, Dielectric, Magnetic, Photocatalytic, and Antibacterial Properties of Ga-Doped BiGaFeO Synthesized by the Microemulsion Approach.

The effect of Ga-substitution on bismuth ferrite BiGaFeO ( = 0, 0.05, 0.10, 0.

Phage-antibiotic synergism against Salmonella typhi isolated from stool samples of typhoid patients.

Background: Typhoid fever is a fatal disease in humans that is caused by Salmonella typhi. S. typhi infections need immediate antibiotic therapy, and their extensive use has led to multidrug-resistant (MDR) pathogens.

Graphene nanoplatelets (GNPs): a source to bring change in the properties of Co-Ni-Gd-ferrite/GNP nanocomposites.

The nanocomposites of CoNiGdFeO/graphene nanoplatelets (CNGF/GNPs) were synthesized by a cost-effective sol-gel auto combustion (SGAC) route. The X-ray diffraction analysis confirmed the cubic structure of the as-prepared nanocomposites, and a crystallite size of 32.28 nm was observed for the 7.

Comprehensive Improvement of Various Features of Cu-Cd Ferrites (CuCdFeCeO) by Cerium (Ce) Ion Substitution.

Cerium (Ce) substitution in Cu-Cd spinel nanoferrites with the compositional formula CuCdFeCeO ( = 0.0, 0.0125, 0.

Corrigendum: Sero-epidemiological study of zoonotic bacterial abortifacient agents in small ruminants.

[This corrects the article DOI: 10.3389/fvets.2023.

Novel insights regarding the safety and efficacy of pyrethroid-coated nanoparticles against ticks.

Nanoparticles have been shown to inhibit major life cycle stages of ticks, indicative of the promising application of nanomaterials against hard ticks. The study thus probed into one of the alternative options to curtail by employing nanocomposites consisting of pyrethroids (cypermethrin and deltamethrin) coated nanoparticles of iron oxides and iron sulfides keeping alongside the evaluation of their toxicity through plant and mammalian cell lines. The nanoparticles used in this study were roughly spherical in morphology and exhibited various size dimensions upon characterization using SEM, EDX, and FTIR.

Sero-epidemiological study of zoonotic bacterial abortifacient agents in small ruminants.

Abortion is one of the leading causes of economic losses in the livestock industry worldwide. , , and spp. are the leading cause of late fetal loss in small ruminants.

Optical, Photocatalytic, Electrochemical, Magnetic, Dielectric, and Ferroelectric Properties of Cd- and Er-Doped BiFeO Prepared via a Facile Microemulsion Route.

A series of Cd- and Er-doped bismuth ferrites were synthesized using a simple microemulsion technique. The influence of Cd and Er doping on the structural, ferroelectric, photocatalytic, and dielectric properties of bismuth ferrite (BFO) was examined in this research. The prepared materials were examined by X-ray diffraction, Raman, scanning electron microscopy, and UV-vis techniques.

Pattern of rifampicin resistance and gene xpert based molecular typing of tuberculosis patients in tertiary care hospitals.

Mycobacterium tuberculosis associated morbidity, mortality and drug resistance is a global health issue. The Gene Xpert is used for early diagnosis of TB and simultaneous detection of Rifampicin (RIF) resistance. We aimed to determine situation analysis of clinical TB in tertiary care hospitals of Faisalabad and to find out frequency of TB and drug resistance pattern by Gene Xpert.

The SHERLOCK Platform: An Insight into Advances in Viral Disease Diagnosis.

Persistence and prevalence of microbial diseases (pandemics, epidemics) is the most alarming threats to the human resulting in huge health and economic losses. Rapid detection and understanding of the disease dynamics by molecular biotechnology tools allow for robust reporting, treatment and control of diseases. As per WHO, the optimal diagnostic approach should be quick, specific, sensitive, without a stringed instrument, and low cost.

PPARγ signaling in hepatocarcinogenesis: Mechanistic insights for cellular reprogramming and therapeutic implications.

Liver cancer or hepatocellular carcinoma (HCC) is leading cause of cancer-related mortalities globally. The therapeutic approaches for chronic liver diseases-associated liver cancers aimed at modulating immune check-points and peroxisome proliferator-activated receptor gamma (PPARγ) signaling pathway during multistep process of hepatocarcinogenesis that played a dispensable role in immunopathogenesis and outcomes of disease. Herein, the review highlights PPARγ-induced effects in balancing inflammatory (tumor necrosis factor alpha (TNF-α), interleukin (IL)-6, IL-1) and anti-inflammatory cytokines (IL-10, transforming growth factor beta (TGF-β), and interplay of PPARγ, hepatic stellate cells and fibrogenic niche in cell-intrinsic and -extrinsic crosstalk of hepatocarcinogenesis.

Distribution and genetic diversity of multi-drug-resistant at the human-animal-environment interface in Pakistan.

Klebsiella pneumoniae is ubiquitous and known to be a notorious pathogen of humans, animals, and plant-based foods. is a recognized trafficker of antibiotic resistance genes (ARGs) between and from different ecological niches. A total of 775 samples ( = 775) were collected from September 2017 to August 2019 from humans, animals, and environmental sources by applying the random convenient sampling technique.

Methotrexate-loaded biodegradable nanoparticles exert anti-arthritic effect by downregulating pro-inflammatory cytokines in Freund's complete adjuvant-induced arthritic rats.

Methotrexate (MTX), the first-line drug for the treatment of rheumatoid arthritis (RA), can cause considerable toxicity, which limits effective dosage regimens. Moreover, it has rapid clearance, which leads to poor patient compliance. To mitigate such challenges, this study aimed to validate the use of MTX-loaded chitosan nanoparticles (NPs) in treating Freund's complete adjuvant (FCA) arthritis in rats.