Publications by authors named "Muhammad Hilman Fu'adil Amin"

Article Synopsis
  • The study introduces a cost-effective eDNA extraction method using guanidine hydrochloride (GuHCl), making it easier to assess aquatic biodiversity, particularly in developing countries.
  • The GuHCl method successfully detected more fish species in freshwater compared to the traditional Qiagen kit, but faced challenges in brackish water due to higher PCR inhibition.
  • Overall, the GuHCl protocol offers a more sensitive and affordable option for large-scale eDNA studies, especially beneficial for monitoring low-abundance species.
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This study offers an in-depth analysis of the mitochondrial genome of (Gill 1863), a species native to the Eastern Atlantic Ocean. The circular mitochondrial DNA molecule measures 16,541 base pairs and comprises 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, two ribosomal RNA genes, and a control region (CR). The nucleotide composition exhibits a notable adenine-thymine (AT) bias, accounting for 53.

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Article Synopsis
  • * Researchers sequenced the complete mitochondrial genome of P. perotaei, revealing 16,691 base pairs that include essential protein-coding genes and other genetic elements.
  • * The study found strong negative selection across fish species in this family and identified unique genetic characteristics useful for studying populations, while also revealing a complex evolutionary relationship among Pomadasys species.
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The global exploration of evolutionary trends in groupers, based on mitogenomes, is currently underway. This research extensively investigates the structure of and variations in species mitogenomes, along with their phylogenetic relationships, focusing specifically on from the Eastern Atlantic Ocean. The generated mitogenome spans 16,572 base pairs and exhibits a gene order analogous to that of the ancestral teleost's, featuring 13 protein-coding genes (PCGs), two ribosomal RNA genes (rRNAs), 22 transfer RNA genes (tRNAs), and an AT-rich control region.

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The mitogenome of an endemic catfish was determined from the Cameroon water. This circular mitogenome was 16,511 bp in length and comprised 13 protein-coding genes, 2 ribosomal RNAs, 22 transfer RNAs, and a single AT-rich control region. The heavy strand accommodates 28 genes, whereas the light strand is constituted by and eight transfer RNA (tRNA) genes.

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A species-specific quantitative PCR (qPCR) assay using environmental DNA (eDNA) is a promising tool for both qualitative and quantitative analyses of target species directly from water samples. Despite its reliability, an eDNA-based qPCR assay pipeline has not yet developed to monitor salmon species inhabiting Korean waters, which have been rapidly decreasing. We designed species-specific primers for four species inhabiting the eastern coastal waters along the Korean Peninsula.

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The complete mitochondrial DNA information of Bleeker, 1863, collected from Sierra Leone was determined using next-generation sequencing (NGS) and bioinfromatic analysis. Its mitogenome (16,504 bp) encoded the typical 13 protein-coding genes (PCGs), 2 ribosomal RNAs (12S & 16S), and 22 tRNAs. All 13 PCGs showed a standard start codon (ATG) but an unusual stop codon (AGA) was identified in gene.

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The first mitochondrial genome of was determined by the combination of next-generation sequencing (NGS) and Sanger sequencing methods. A complete circular mitogenome of (16,529 bp) consisted of 13 protein-coding genes, 22 transfer RNAs, two ribosomal RNAs, and two non-coding regions, including a control region (D-loop) and a light strand origin of replication (O). Two start codons (ATG and GTG) and four stop codons (TAG, TAA, TA-, and T-) were used in all the PCGs.

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We applied next-generation sequencing (NGS) method to construct the complete mitochondrial genome of . The obtained mitogenome of (16,590 bp) exhibited a typical structure harboring 13 protein-coding genes (PCGs), 22 transfer RNAs (tRNAs), two ribosomal RNAs (rRNAs), and one control regions (D-loop). Most of mitochondrial genes are encoded on the heavy (H) strand, except for eight tRNAs and ND6.

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