Publications by authors named "Muhammad Hanafiah"

Background And Aim: The capa plant ( L.) has been widely used as a traditional herbal medicine in many parts of the world, including South Aceh, Indonesia. It is generally used for wound healing due to its antibacterial and anti-inflammatory properties.

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Aim: The objective of this study was to produce recombinant protein GRA-4 (rGRA-4) of a local isolate as a candidate for a toxoplasmosis diagnosis kit in BL21 (DE3) competent cells using pET SUMO plasmid.

Materials And Methods: Samples used were stock tachyzoites DNA from the Parasitology Laboratory, Faculty of Veterinary Medicine, Gadjah Mada University, Yogyakarta. Amplified GRA-4 polymerase chain reaction product of tachyzoite DNA was cloned in the pET-SUMO TA cloning vector.

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Aim: The objective of this research was to identify the parasite species found in the gastrointestinal tract and pancreas of Aceh cattle slaughtered in a Banda Aceh slaughterhouse using lactophenol and semichon carmine staining.

Materials And Methods: Each sample out of 50 samples of gastrointestinal tract and pancreas from Aceh cattle slaughtered in a Banda Aceh slaughterhouse was separated by organ. Each organ was examined for the presence of worm.

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Aim: The aim of this research was to determine the copro-prevalence of using polymerase chain reaction (PCR) with repetitive 529 bp gene and to construct the phylogenetic tree of oocyst from pet cats in Yogyakarta.

Materials And Methods: 9 of 132 pet cat samples which serologically positive for were used in this research. To determine the copro-prevalence of in pet cat, 10 g of feces samples taken from practitioners and household cats in Yogyakarta were used in the PCR method utilizing repetitive 529 bp gene sequences.

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Aim: The aims of the study are to detect the presence of antigen and to determine its distribution location in several organs of domestic cat using immunohistochemistry (IHC) method with Labeled-[Strept] Avidin-Biotin (LAB-SA).

Material And Methods: Four domestic cats aged 1-2 years were used as sample in this research. The sample divided into two groups with two cats each.

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