Mechanism assay of interaction between blood vessels-near infrared probe and cell surface marker proteins of endothelial cells.

blood vessels imaging is crucial to study blood vessels related diseases in real-time. For this purpose, fluorescent based imaging is one of the utmost techniques for imaging a living system. The discovery of a new near-infrared probe (CyA-B2) by screening chemical probe library in our previous report which showed the most specific binding on the blood capillaries of the 3D-tissue models give us interest to study more about the binding site of this probe to the surface of endothelial cells main component cell of blood capillaries.

A Near-Infrared Organic Fluorescent Probe for Broad Applications for Blood Vessels Imaging by High-Throughput Screening via 3D-Blood Vessel Models.

Blood vessels are present in all of the organs, reflecting their importance for oxygen and nutrient delivery to the cells. Until now, no organic fluorophore has been reported for the live imaging of endothelium although the layer is the key to blood vessel functions. Here, the discovery of a blood vessel organic probe at near-infrared (NIR) wavelength range (BV-NIR) through an engineered blood capillary-based screening system, which is a more physiological model than a conventional cell culture condition, is reported.

Fabrication of Blood Capillary Models for Live Imaging Microarray Analysis.

Conventional microarray analysis usually deals with the monolayer or two-dimensional (2D) assays for the high-throughput screening applications. Even though these cell-based assays are effective for preliminary screening at least to have information on cytotoxicity, they do not adequately re-create the in vivo complexity of three-dimensional (3D) tissues. In this study, 3D-blood capillary models were constructed by using physiological collagen microfibers (CMF), which provide the extracellular matrix in the complex tissue.