Mutational analysis of hemoglobin genes and functional characterization of detected variants, through in-silico analysis, in Pakistani beta-thalassemia major patients.

Thalassemia is one of the most prevalent genetic disorders worldwide. The present study aimed to explore the mutational spectrum of all hemoglobin (HB) encoding genes and to identify the potentially damaging and pathogenic variants in the beta (β)-thalassemia major patients and thalassemia minor carriers of Southern Punjab, Pakistan. A total of 49 β-thalassemia major patients and 49 carrier samples were screened for the identification of HBA1, HBA2, HBB, HBD, HBE1, HBG1 and HBG2 variants by NGS.

Cytokinin Production by Contributes to Increase in Growth, Yield, Antioxidant, and Physiological Systems of Wheat ( L.).

Plant growth-promoting rhizobacteria are known to associate with several cereal crops. The rhizobacterium exerts its function by synthesizing diverse arrays of phytohormones, such as cytokinin (Ck). However, it is difficult to determine the plant growth promotion when a bacterium produces many different kinds of phytohormones.

Positive Selection Drives the Adaptive Evolution of Mitochondrial Antiviral Signaling (MAVS) Proteins-Mediating Innate Immunity in Mammals.

The regulated production of filamentous protein complexes is essential in many biological processes and provides a new paradigm in signal transmission. The mitochondrial antiviral signaling protein (MAVS) is a critical signaling hub in innate immunity that is activated when a receptor induces a shift in the globular caspase activation and recruitment domain of MAVS into helical superstructures (filaments). It is of interest whether adaptive evolution affects the proteins involved in innate immunity.

Public knowledge, attitudes, and practices towards COVID-19 in Pakistan: A cross-sectional study.

In response to the recent COVID-19 pandemic, each country has adopted different measures for its control, for instance, complete lockdown, smart lockdown based on hotspot analysis, and/or strict movement control on their residents. The effectiveness of such controls depends largely on public knowledge, attitude, and practices towards this novel virus. The prime objective of this study is to examine the knowledge, attitudes, and practices of university students and graduates in Pakistan regarding COVID-19.