Testing intravitreal toxicity of rapamycin in rabbit eyes.

Purpose: To evaluate retinal toxicity of varying doses of rapamycin when injected intravitreally in rabbits. Rapamycin is a potent immunosuppressive agent with significant antitumor and antiangiogenic properties, clinically approved for prevention of organ transplant rejection.

Methods: Twelve New Zealand albino rabbits were divided into four groups.

Intravitreal toxicity of ketorolac tris salt and flurbiprofen.

Background And Objective: To determine the retinal toxicity of intravitreal ketorolac tris salt and flurbiprofen.

Materials And Methods: Thirty-two New Zealand rabbits were injected intravitreally with 125, 250, or 500 microg or 1 mg of ketorolac tris salt or flurbiprofen in one eye and 8 fellow eyes were injected with 5% dextrose as a control. All animals underwent indirect ophthalmoscopy and slit-lamp biomicroscopy before injection and on days 1, 7, and 14 after the intravitreal injection.

Inhibition of experimental angiogenesis of cornea by various doses of doxycycline and combination of triamcinolone acetonide with low-molecular-weight heparin and doxycycline.

Purpose: To evaluate the effect of topically administered doxycycline in various doses; the combination of triamcinolone acetonide and low-molecular-weight heparin (LMWH); and the combination of triamcinolone acetonide and doxycycline on experimental corneal neovascularization in rats.

Methods: This project is the combination of 2 separate studies. First, the chemical cauterization of corneas in 36 eyes of 36 Long Evans male rats was performed by using silver nitrate/potassium nitrate sticks.

Ocular toxicity of intravitreous adalimumab (Humira) in the rabbit.

Purpose: To evaluate the ocular toxicity of escalating doses of intravitreous adalimumab (Humira) in the rabbit eye.

Methods: Twelve New Zealand albino rabbits received unilateral intravitreous injections of 0.1 ml of adalimumab 0.

Threshold parameters for choroidal vessel closure with the photosensitizer, lambda 27.

Objective: To determine the threshold photodynamic therapy parameters, required for choroidal vessel closure, with the photosensitizer, Lambda 27, in Dutch belted rabbits, using fluorescein angiography and histopathology.

Design: A pre-clinical experiment.

Place And Duration Of Study: Department of Ophthalmology at the Tulane University Health Sciences Center, between June 2001 - July 2002.

The effect of intravitreally injected povidone-iodine on Staphylococcus epidermidis in rabbit eyes.

Purpose: Firstly, the aim of this study was to determine the maximally tolerated dose of intravitreally injected povidone-iodine (PVP-I). A second aim was to test the efficacy of PVP-I on rabbit eyes infected intravitreally with Staphylococcus epidermidis.

Methods: Phase I of the study used 16 New Zealand albino rabbits, divided into 4 groups (n=4 each).

Intravitreal toxicity of high-dose etanercept.

Purpose: The aim of this study was to evaluate the retinal toxicity of high-dose intravitreal etanercept, a U.S. Food and Drug Administration-approved anti-inflammatory drug, in the rabbit model.

Inhibition of corneal angiogenesis by ascorbic acid in the rat model.

Purpose: To evaluate the effect of topically administered ascorbic acid on experimentally induced corneal neovascularization in the rat model.

Materials And Methods: Corneal chemical cauterization of 72 eyes in Long-Evans male rats was performed using silver nitrate/potassium nitrate sticks. Nine groups of eight eyes were used to evaluate eight concentrations of ascorbic acid with one group of eight eyes serving as a control.

Inhibition of experimental corneal neovascularisation by bevacizumab (Avastin).

Aim: To evaluate the effect of topically administered bevacizumab (Avastin) on experimental corneal neovascularisation in rats.

Methods: Silver nitrate sticks (75% silver nitrate, 25% potassium nitrate) were used to perform chemical cauterisation on the corneas of 16 eyes from 16 male Long Evans rats. For the following 7 days, the 10 eyes in the treatment group were instilled with bevacizumab 4 mg/ml drops twice daily, whereas the 6 eyes in the control group received placebo (normal saline drops twice daily).

Prevention of corneal neovascularization: evaluation of various commercially available compounds in an experimental rat model.

Purpose: To evaluate the effect of topically administered agents doxycycline, triamcinolone, low molecular weight heparin (LMWH), and flurbiprofen on experimental corneal neovascularization in rats.

Methods: Chemical cauterization of cornea was performed in 36 eyes of 36 rats by using silver nitrate/potassium nitrate sticks. Topical instillation of doxycycline, triamcinolone, LMWH, flurbiprofen, and normal saline solution was continued for 7 days.

The effect of combinations of flurbiprofen, low molecular weight heparin, and doxycycline on the inhibition of corneal neovascularization.

Purpose: To determine the efficacy of various combinations of flurbiprofen, low molecular weight heparin (LMWH), and doxycycline on the inhibition of corneal neovascularization in rats.

Methods: Chemical cauterization of the cornea in 32 eyes of 32 rats was performed using silver nitrate/potassium nitrate sticks. The animals were divided into 4 groups.

Retinal toxicity of intravitreal 5-fluorouracil in silicone-filled rabbit eyes.

Objective: To determine the retinal toxicity of 5-fluorouracil, by means of electroretinography and histopathology, at different doses in vitrectomized rabbit eyes injected with silicone oil.

Design: A pre-clinical experimental study.

Place And Duration Of Study: The Louisiana State University Eye Center and the Department of Ophthalmology at the Tulane University Health Sciences Center, between June 1999 and May 2002.

Absorption of diode 810 nm used for transpupillary thermotherapy by blood. An in vitro study.

Background And Objectives: To evaluate the in vitro energy attenuation by transpupillary thermotherapy (TTT) using an 810-nm diode laser after the treatment of exudative age-related macular degeneration.

Study Design/materials And Methods: In this experiment, an 810-nm diode laser was coupled to a slit lamp to deliver laser energy to a laser-power sensor adjusted in the track of the laser light. The spotlight was 0.

Testing intravitreal toxicity of bevacizumab (Avastin).

Purpose: To evaluate the retinal toxicity of varying doses of bevacizumab when injected intravitreally in rabbits. Bevacizumab has been approved by the US Food and Drug Administration for the treatment of metastatic colorectal cancer.

Materials And Methods: Twelve New Zealand albino rabbits were used for this study and divided into four groups.