Con-DAM: Simultaneous measurement of food intake and sleep in at the single fly resolution.

Sleep and feeding are conserved behaviors across many taxa of the animal kingdom and are essential for an organism's survival and fitness. Although has been used to study these behaviors for decades, concurrent measurement of these two behaviors in the same flies on solid media has been a challenge. Here, we report Con-DAM, which enables simultaneous quantification of food intake and sleep/activity at the single fly resolution.

Dietary restriction fails to extend lifespan of Drosophila model of Werner syndrome.

Werner syndrome (WS) is a rare genetic disease in humans, caused by mutations in the WRN gene that encodes a protein containing helicase and exonuclease domains. WS is characterized by symptoms of accelerated aging in multiple tissues and organs, involving increased risk of cancer, heart failure, and metabolic dysfunction. These conditions ultimately lead to the premature mortality of patients with WS.

Con-FLIC: concurrent measurement of feeding behaviors and food consumption in at single-fly resolution.

Accurate quantification of food intake and feeding behaviors are essential for understanding various biological processes, such as metabolism and aging. Currently, no methods allow for the concurrent measurement of both parameters for the same individual flies. Here, we couple Con-Ex ( Con sumption- Ex cretion) and FLIC ( F ly L iquid-Food I nteraction C ounter), previously developed to measure food consumption and various feeding behaviors, respectively, into a single platform that we named Con-FLIC.