Anti-biofilm activity of a novel nanoemulsion containing Curcuma xanthorrhiza oil.

Objectives: We aimed to solubilize Curcuma xanthorrhiza oil (CXO) using nanoemulsification and evaluate its inhibitory effects against biofilm formation.

Methods: The components of CXO were evaluated through high-performance liquid chromatography (HPLC) analysis. Healthy human saliva was inoculated onto hydroxyapatite discs to form microcosm biofilms for four days and treated six times with each antimicrobial agent: distilled water (DW), CXO emulsion (EM), CXO nanoemulsion (NE), and positive controls (Listerine and chlorhexidine).

Antimicrobial activity of nanoemulsions on biofilms.

In this study, an optimal nanoemulsion formulation for oil (Xan) was investigated using different sonication times. The antimicrobial effects of the nanoemulsion, the original emulsion, distilled water (DW), and Listerine, on biofilms were compared. The optimum ultrasonic time, determined in terms of droplet size and stability, was found to be 10 min.

Effects of short-time exposure of surface pre-reacted glass-ionomer eluate on dental microcosm biofilm.

This study evaluated the antibacterial effects of short-time exposure of surface pre-reacted glass-ionomer (S-PRG) eluate on oral microcosm biofilm. Biofilms were treated with an S-PRG eluate at different concentrations (25%, 50%, and 100%), distilled water (DW), and 0.1% chlorhexidine (CHX) twice a day for 5 min repeatedly.