Reducing the virulence of Pseudomonas aeruginosa by using multiple quorum-quenching enzymes.

Unlabelled: The emergence of multidrug-resistant Pseudomonas aeruginosa in healthcare settings poses a tremendous challenge to traditional antibiotic therapy. Pseudomonas aeruginosa utilizes quorum sensing (QS) to coordinate the production of virulence factors and the formation of drug-resistant biofilms. QS is mediated by signal compounds produced by P.

Bacterial Consortium-Based Sensing System for Detecting Organophosphorus Pesticides.

Engineered bacteria with synthetic gene circuits are attractive tools to detect environmental contaminants. However, their applications in realistic settings are hindered by its relatively low sensitivity, long response time, and limited portability. Here, we present a synthetic bacterial consortium-based system for detecting organophosphorus pesticides (OPs).

Stepwise Loop Insertion Strategy for Active Site Remodeling to Generate Novel Enzyme Functions.

The remodeling of active sites to generate novel biocatalysts is an attractive and challenging task. We developed a stepwise loop insertion strategy (StLois), in which randomized residue pairs are inserted into active site loops. The phosphotriesterase-like lactonase from Geobacillus kaustophilus (GkaP-PLL) was used to investigate StLois's potential for changing enzyme function.