Antifungal activity of human antimicrobial peptides targeting apoptosis in .

Innovative antifungal therapies are of crucial importance to combat the potentially life-threatening infections linked to the multidrug-resistant fungal pathogen . Induction of regulated cell death, apoptosis, could provide an outline for future therapeutics. Human antimicrobial peptides (AMPs), well-known antifungal compounds, have shown the ability to induce apoptosis in pathogenic fungi.

Comparison of PetrifilmTM AC and pour plate techniques used for the heterotrophic aerobic bacterial count in water.

Heterotrophic bacteria are commonly found in water samples. While these Heterotrophic Bacterial/Plate Counts (HPC) do not necessarily indicate a health hazard, high counts provide a good indication of the efficiency of water disinfection and integrity of distribution systems. The aim of this study was to compare the PetrifimTM AC method to the pour plate technique for the testing of HPC in water samples.

Fungicidal activity of human antimicrobial peptides and their synergistic interaction with common antifungals against multidrug-resistant Candida auris.

Emergence of Candida auris, a multidrug-resistant yeast, demonstrates the urgent need for novel antifungal agents. Human antimicrobial peptides (AMPs) are naturally occurring molecules with wide spectrum antimicrobial activity, particularly against a variety of fungi. Therefore, this study examined the antifungal activity of seven different human AMPs against C.

Oral Cavity and : Colonisation to the Development of Infection.

colonisation of the oral cavity increases in immunocompromised individuals which leads to the development of oral candidiasis. In addition, host factors such as xerostomia, smoking, oral prostheses, dental caries, diabetes and cancer treatment accelerate the disease process. is the primary causative agent of this infection, owing to its ability to form biofilm and hyphae and to produce hydrolytic enzymes and candialysin.

Tolerance of Listeria monocytogenes to biocides used in food processing environments.

Listeria monocytogenes is a foodborne pathogen that causes a life-threatening disease in humans known as listeriosis. Contamination of food during processing is the main route of transmission of Listeria monocytogenes. Therefore, biocides play a crucial role in food processing environments as they act as the first line of defense in the prevention and control of L.

Infection control in dentistry during COVID - 19 pandemic: what has changed?

The novel coronavirus (COVID-19) pandemic has emerged disrupting many socio-economical and healthcare aspects across the world. This virus can be transmitted by symptomatic and asymptomatic individuals through saliva and contact. Due to its airborne transmission, aerosols created by natural activities and during dental treatment of infected individuals have become a potential vehicle of transmission and threat.

Mezoneuron benthamianum inhibits cell adherence, hyphae formation, and phospholipase production in Candida albicans.

The aim of this study was to evaluate the phytochemical constituents, antioxidant, antifungal, and anti-virulence activities of traditionally used Mezoneuron benthamianum leaves. Extracts were prepared using acetone and methanol, and the preliminary phytochemical screening was performed. The antioxidant activity was studied using the DPPH method.

Dodonaea viscosa var angustifolia derived 5,6,8-trihydroxy-7,4' dimethoxy flavone inhibits ergosterol synthesis and the production of hyphae and biofilm in Candida albicans.

Ethnopharmacological Relevance: Candida albicans is developing resistance to existing drugs increasing morbidity and mortality, which elevates an immediate need to explore new antifungal agents. Phytochemicals are an excellent source of therapeutic agents. We previously reported the antifungal activity of the crude extract of Dodonaea viscosa var.

Quantitative Analysis of Selected Microorganisms Present at Various Sites in a Prosthetics Clinic and Dental Laboratory during Complete Denture Fabrication.

: Contamination with oral commensals and pathogenic microorganisms, and cross contamination between clinic and laboratory can occur. The amount of contamination has not been determined. : Samples from different clinical and laboratory stages before and after disinfection (17 sites, 10 samples per stage) were collected.

Improved efficacy of antifungal drugs in combination with monoterpene phenols against Candida auris.

Emergence of Candida auris has been described as a global health threat due to its ability to cause invasive infections with high mortality rate and multidrug resistance. Novel drugs and therapies are required to target this organism and its pathogenicity. Anti-virulence approach and combination therapy have been proposed as alternatives in recent years.

Anti-acidogenic, anti-biofilm and slow release properties of Dodonaea viscosa var. angustifolia flavone stabilized polymeric nanoparticles.

Objective: Dental caries is caused by plaque associated oral bacteria including a pioneer species Streptococcus mutans. It has ability to form biofilm and produce acids in the oral cavity. Many oral hygiene products containing plant derived compounds have been investigated for their anti-S.

Viability of Candida albicans in Denture Base Resin After Disinfection: A Preliminary Study.

Purpose: To quantify the depth of penetration of Candida albicans (C albicans) into a denture base resin and to investigate its viability after disinfection.

Materials And Methods: Heat-polymerizing polymethyl methacrylate plates were contaminated with C albicans, then washed, dried, and prepared for scanning electron microscopy (SEM). Vertical surfaces were cut, and the depth of any penetration was measured.

Synergistic antifungal effect of cyclized chalcone derivatives and fluconazole against .

The occurrence of invasive fungal diseases, particularly in immunocompromised patients, is life-threatening and increases the economic burden. The rising problem of multi-drug resistance is becoming a major concern for clinicians. In addition, a repertoire of antifungal agents is far less in number than antibacterial drugs.

Anti-acidogenic and anti-biofilm activity of 5,6,8-trihydroxy-7-methoxy-2-(4-methoxyphenyl)-4H-chromen-4-one.

Objectives: Crude extracts of Dodonaea viscosa var. angustifolia (DVA), has shown to have anticariogenic property. However the compound responsible for this activity has not been identified.

Effect of Punica granatum on the virulence factors of cariogenic bacteria Streptococcus mutans.

Dental caries is caused by acids produced by biofilm-forming Streptococcus mutans from fermentable carbohydrates and bacterial byproducts. Control of these bacteria is important in the prevention of dental caries. This study investigated the effect of the fruit peel of Punica granatum on biofilm formation, acid and extracellular polysaccharides production (EPS) by S.

Challenges in the Development of Antifungal Agents Against Candida: Scope of Phytochemical Research.

Background: Infections caused by Candida have become a major source of morbidity and mortality. Limited numbers of drugs are available to treat these infections. Phytochemicals can be the major source of antifungal compounds.

Detection of cfxA2, cfxA3, and cfxA6 genes in beta-lactamase producing oral anaerobes.

Purpose The aim of this study was to identify β-lactamase-producing oral anaerobic bacteria and screen them for the presence of cfxA and BlaTEM genes that are responsible for β-lactamase production and resistance to β-lactam antibiotics. Material and Methods Periodontal pocket debris samples were collected from 48 patients with chronic periodontitis and anaerobically cultured on blood agar plates with and without β-lactam antibiotics. Presumptive β-lactamase-producing isolates were evaluated for definite β-lactamase production using the nitrocefin slide method and identified using the API Rapid 32A system.

The efficacy of disinfectants in the decontamination of dental unit water lines: an laboratory study.

Objectives/aims: This laboratory study compared the efficacy of water, sodium percarbonate (SPC) and chlorine dioxide (ClO) solutions in the disinfection of dental unit water lines (DUWLs).

Materials And Methods: New DUWL tubes were cut, split open, and mono-culture and mixed-culture biofilms of , and were grown. Harvested biofilms from the sectioned DUWL tubes were exposed to sterile distilled water, SPC or 5 and 10 p.

Influence of cancer treatment on the Candida albicans isolated from the oral cavities of cancer patients.

Purpose: Cancer treatment causes mucositis and the manifestation of oral candidiasis. This study investigated the virulence properties and antifungal susceptibilities of Candida albicans isolated from cancer patients undergoing therapy.

Methods: C.

Virulence of oral Candida isolated from HIV-positive women with oral candidiasis and asymptomatic carriers.

Objective: This study compared the virulence of oral Candida species isolated from human immunodeficiency virus (HIV)-positive women with and without oral candidiasis.

Study Design: Candida species were isolated from 197 women, and their virulence attributes were measured.

Results: Of the 197 women, 117 (59.

Dodonaea viscosa var. angustifolia Inhibits Germ Tube and Biofilm Formation by C. albicans.

The virulence factors of Candida albicans are germ tube and biofilm formation, adherence to host tissues, and production of hydrolytic enzymes. This study investigated the effect of Dodonaea viscosa var. angustifolia extract on the germ tube and biofilm formation of C.

Inhibitory Effect of Dodonaea viscosa var. angustifolia on the Virulence Properties of the Oral Pathogens Streptococcus mutans and Porphyromonas gingivalis.

Aim. This study investigated the effect of Dodonaea viscosa var. angustifolia (DVA) on the virulence properties of cariogenic Streptococcus mutans and Porphyromonas gingivalis implicated in periodontal diseases.

Oral Candida colonization in HIV-positive women: associated factors and changes following antiretroviral therapy.

There is a paucity of information about the factors associated with oral colonization with Candida species and the changes associated with antiretroviral (ARV) therapy. This study investigated the role of ARV therapy and other factors in a study population. Relevant clinical and laboratory information was obtained and oral rinse specimens were tested for yeast identification.

Inhibitory activity of Dodonaea viscosa var. angustifolia extract against Streptococcus mutans and its biofilm.

Ethnopharmacological Relevance: The leaves of Dodonaea viscosa var. angustifolia (DVA) are traditionally used for the treatment of fever, colds, oral thrush, toothaches and related problems. Streptococcus mutans is implicated in many oral infections.

Isolation of Staphylococcus aureus and black-pigmented bacteroides indicate a high risk for the development of Ludwig's angina.

Objective: The aim of this study was to compare clinical, microbiological, enzymatic, and host immune response variables between subjects hospitalized with facial cellulitis, with Ludwig's angina (LA) and without Ludwig's angina (WOLA).

Study Design: Microbiological and enzymatic tests on pus, and hematological and immunological assessments on blood samples of 15 patients with LA and 42 patients with WOLA were performed. Laboratory findings of both groups were compared using the Student t test.