[Isolation and various properties of leucine aminopeptidase from biological fluids of patients with multiple sclerosis].

Specific form of leucine aminopeptidase (which was distinct from other forms of the enzyme found in blood) was characterized by its physico-chemical properties--pH optimum, substrate specificity, electrophoretic mobility and molecular mass. The enzyme was isolated from biological fluids of patients with multiple sclerosis. Free and bound forms of the enzyme were detected in blood and cerebrospinal fluid.

[Phosphorus and calcium metabolism and its hormonal regulation in chronic kidney failure].

In order to elucidate the blood serum calcium, inorganic phosphorus, parathyroid hormone (PTH) and calcitonin (CT) content in the course of the development of chronic renal failure (CRF), 80 patients with chronile glomerulonephritis were examined. Of these, in 24 glomerular filtration (GF) was normal, whereas 56 had CRF of varying degree. Thirty-three out of the 80 patients had the nephrotic syndrome (NS).

[Kinetic method for determination of isoenzymes of alkaline phosphatase in human blood serum].

Resistance of liver, bone, intestinal and placental human alkaline phosphatase isozymes to the effect of L-phenylalanine and urea was studied. The combined treatment with urea and L-phenylalanine increased an accuracy of estimation due to inhibition of bone and intestinal isozymes. A special equation enabled to simplify the calculations.