The diversity of bacteria isolated from antarctic freshwater reservoirs possessing the ability to produce polyhydroxyalkanoates.

The diversity of polyhydroxyalkanoates-producing bacteria in freshwater reservoirs in the Ecology Glacier foreland, Antarctica, was examined by a cultivation-dependent method. Isolated strains were analyzed phylogenetically by 16S rRNA gene sequencing, and classified as members of Alpha-, Beta-, or Gammaproteobacteria classes. Polymerase chain reaction was used to detect PHA synthase genes.

Pulsed feeding strategy is more favorable to medium-chain-length polyhydroxyalkanoates production from waste rapeseed oil.

This article presents the results of production and characterization of medium-chain-length polyhydroxyalkanoates (mcl-PHAs) using Pseudomonas sp. Gl01. Studies have been carried out to find suitable feeding strategies for mcl-PHAs production and, for the first time, to investigate in-depth the properties of biopolyesters obtained under controlled conditions with waste rapeseed oil as a substrate.

Molecular identification of polyhydroxyalkanoates-producing bacteria isolated from enriched microbial community.

Polyhydroxyalkanoates (PHAs) are especially interesting because of their similar properties to synthetic plastics and their potential use as biodegradable polymers. Many strategies have been employed to effectively and economically produce PHAs, among them a production process based on mixed microbial populations, enriched from activated sludge could be one of the alternative technologies. Defining the bacterial species creating these anonymous populations is crucial for the improvement of cultivation strategy.

Saponified waste palm oil as an attractive renewable resource for mcl-polyhydroxyalkanoate synthesis.

The synthesis of mcl-polyhydroxyalkanoates (mcl-PHAs) by Pseudomonas sp. Gl01 using saponified waste palm oil (SWPO) as the sole carbon source was investigated. It was shown that the analyzed strain accumulated biopolymers during the growth phase.

Ribosomal intergenic spacer analysis as a tool for monitoring methanogenic Archaea changes in an anaerobic digester.

The applicability of a newly-designed PCR primer pair in examination of methanogenic Archaea in a digester treating plant biomass was evaluated by Ribosmal Intergenic Spacer Analysis (RISA). To find a suitable approach, three variants of RISA were tested: (1) standard, polyacrylamide gel-based, (2) automated, utilized capillary electrophoresis (GA-ARISA), and (3) automated microfluidics-based (MF-ARISA). All three techniques yielded a consistent picture of archaeal community structure changes during anaerobic digestion monitored for more than 6 weeks.

Mcl-PHAs produced by Pseudomonas sp. Gl01 using fed-batch cultivation with waste rapeseed oil as carbon source.

The present study describes medium-chain-length polyhydroxyalkanoates (mcl-PHAs) production by the Pseudomonas Gl01 strain isolated from mixed microbial communities utilized for PHAs synthesis. A two-step fedbatch fermentation was conducted with glucose and waste rapeseed oil as the main carbon source for obtaining cell growth and mcl-PHAs accumulation, respectively. The results show that the Pseudomonas Gl01 strain is capable of growing and accumulating mcl-PHAs using a waste oily carbon source.

The influence of nitrogen limitation on mcl-PHA synthesis by two newly isolated strains of Pseudomonas sp.

The nucleotide composition of key enzymes involved in medium-chain-length polyhydroxyalkanoates (mcl-PHA) synthesis was analyzed in two newly isolated strains of Pseudomonas. The isolated strains were tested for their abilities to synthesize polyhydroxyalkanoates using three different substrates as a carbon source: sodium octanoate, oleic acid, and sodium gluconate. Both analyzed strains were able to accumulate mcl-PHA in a range from 2.

Changes in microbial community structure during adaptation towards polyhydroxyalkanoates production.

Polyhydroxyalkanoates (PHAs) are interesting as material for bioplastic production because they are recognized as biodegradable and could be produced from renewable resources. The industrial production of PHAs has already been used in practice by pure cultures. In recent years, many studies have been addressed of PHA production by mixed cultures.

S1 nuclease hydrolysis of single-stranded nucleic acids with partial double-stranded configuration.

The single-strand specific nuclease S1 from Aspergillus oryzae (EC 3.1.4.