Serotonergic elements in the nervous system of parasite of acipenserid fishes, Acrolichanus auriculatus (Digenea: Allocreadiidae).

The trematode Acrolichanus auriculatus is a widely distributed intestine parasite of acipenserid fishes. For the first time the localization and distribution of the serotonergic nerve elements in A. auriculatus was studied using immunocytochemical method and confocal laser scanning microscopy.

Neuromuscular system of the causative agent of dicrocoeliosis, Dicrocoelium lanceatum. II. Neuropeptide FMRFamide immunoreactivity in nervous system.

The parasitic flatworm, trematoda Dicrocoelium lanceatum or lancet fluke is the causative agent of a widespread parasite disease of grazing ruminants, dicrocoeliosis. The aim of this work is the study of the presence and localization of neuropeptide FMRFamide immunoreactive elements in the nervous system of D. lanceatum using immunocytochemical technique and confocal scanning laser microscopy.

Neuromuscular system of the causative agent of dicrocoeliosis, Dicrocoelium lanceatum. I. 5-Hydroxytryptamine in the nervous system.

The trematode Dicrocoelium lanceatum known as lancet fluke, is a causative agent of dicrocoeliosis, a widespread parasitic disease of the grazing ruminants. The investigation of the major neurotransmitters and their functions are an important step in the development of a new pharmacological strategy of the struggle against the dicrocoeliosis affecting the neuronal signal substances and the functions of its nervous system. The aim of this work was to study the presence and localization of the neurotransmitter serotonin (5-HT, 5-Hydroxytryptamine) in the nervous system of D.

Musculature and neurotransmitters of internal organs of trematodes (the digestive, reproductive and excretory systems).

The article analyzes the results on the presence and organization of the muscle elements in the visceral organs of parasitic flatworms, trematodes, as well as their innervations. The different regions of the digestive, reproductive and excretory systems of trematodes contain circular, longitudinal and diagonal muscle fibers. The results of immunocytochemical investigations and confocal scanning laser microscopy show the presence of serotonin and FMRFamide-like immunoreactivity in the nervous system elements in various parts of the digestive, reproductive and excretory systems of trematodes.

An in vitro vascularized micro-tumor model of human colorectal cancer recapitulates in vivo responses to standard-of-care therapy.

Around 95% of anti-cancer drugs that show promise during preclinical study fail to gain FDA-approval for clinical use. This failure of the preclinical pipeline highlights the need for improved, physiologically-relevant in vitro models that can better serve as reliable drug-screening and disease modeling tools. The vascularized micro-tumor (VMT) is a novel three-dimensional model system (tumor-on-a-chip) that recapitulates the complex human tumor microenvironment, including perfused vasculature, within a transparent microfluidic device, allowing real-time study of drug responses and tumor-stromal interactions.

Biodiversity of lung helminths in terrestrial mammals from Eastern Europe.

This review provides the results of many years of original studies of the authors published in different journals and compilations taking into account an analysis of literature data. The studies on biological diversity of lung helminths of terrestrial mammals were performed by the authors in Armenia, Bulgaria and Poland. Literature data on European Russia were also taken into account.

The neuroactive substances and associated muscle system in Rhipidocotyle campanula (Digenea, Bucephalidae) from the intestine of the pike Esox lucius.

We report about the muscular system and the serotonergic and FMRFamidergic components of the nervous system of the Bucephalidae trematode, Rhipidocotyle campanula, an intestinal parasite of the pike. We use immunocytochemical methods and confocal scanning laser microscopy (CLSM). The musculature is identified by histochemical staining with fluorescently labeled phalloidin.

The New Data on the Serotonin and FMRFamide Localization in the Nervous System of Opisthorchis felineus Metacercaria.

Background: Trematoda Opisthorchis felineus Rivolta, 1884 is the causative agent of dangerous parasite disease-opisthorchiasis, widespread in the Russian Federation. The details of the neuroanatomical localization of the serotoninergic and FMRFamidergic neurotransmitter elements as well as their functional roles remain not studied enough in both adult and larval forms of O. felineus.

Nematodes of birds of Armenia.

The review provides data on species composition of nematodes in 50 species of birds from Armenia (South of Lesser Caucasus). Most of the studied birds belong to Passeriformes and Charadriiformes orders. One of the studied species of birds (Larus armenicus) is an endemic.

Species diversity of nematodes in domestic and wild ruminants of Armenia.

The review provides data on species composition of nematode parasites of cattle, sheep, goats and wild ruminants (Ovis orientalis gmelini, Capreolus capreolus, Capra aegagrus aegagrus) of Armenia. Six species of lung nematodes and 22 species of gastrointestinal ones have been registered in ruminants of Armenia. Five of the species listed in the review (Protostrongylus davtiani, Protostrongylus muraschkinzewi, Nematodirus davtiani, Trichostrongylus andreevi, Trichostrongylus skrjabini) have been first identified in Armenia.

Nematodes of rodents of Armenia.

This review summarizes known data on the species composition of nematodes in 24 species of rodents inhabiting the territory of Armenia (South of Lesser Caucasus). The studied rodents include members of some cosmopolitan species (Mus musculus, Rattus norvegicus, Rattus rattus), as well as endemic and narrow-areal species (Meriones vinogradovi, Spermophilus xanthoprymnus, Microtus schidlovskii, Mesocricetus brandti, Sciurus anomalus). The taxonomical identity of the nematode species reported by previous authors is discussed with regard to present knowledge about helminth fauna of rodents.

Serotonin and Neuropeptide FMRFamide in the Attachment Organs of Trematodes.

The serotoninergic and FMRFamidergic nervous system of the attachment organs of trematodes were examined using immunocytochemical techniques and confocal scanning laser microscopy. Adult trematodes from eight families as well as cercariae and metacercariae from ten families were studied. TRITC-conjugated phalloidin was used to stain the muscle fibres.

The epidemiology of trichinellosis in the Arctic territories of a Far Eastern District of the Russian Federation.

Trichinellosis, a zoonotic disease caused by nematodes of the genus Trichinella, is still a public health concern in the Arctic. The aims of this study were to investigate the seroprevalence of anti-Trichinella IgG in aboriginal peoples of two settlements in the Chukotka Autonomous Okrug (Russian Federation) on the Arctic coast of the Bering Sea, and to evaluate the survival of Trichinella nativa larvae in local fermented and frozen meat products. A seroprevalence of 24.