Molecular epidemiology of the Old World screwworm fly (OWSF) in Iraq, and the genetic structure of various OWSF populations worldwide.

Despite being endemic in Iraq, no reports have been published in the past 10 years to update the molecular epidemiology of the Old World screwworm fly (OWSF), Chrysomya bezziana, in this country. In the present study, 130 sheep from 10 Iraqi governorates were found infected with C. bezziana larvae, whose identities were PCR-confirmed based on the cytochrome b (Cytb) gene, and 23 isolates from various tested governorates were successfully sequenced.

Gastrointestinal parasites of cats in Egypt: high prevalence high zoonotic risk.

Background: Several gastrointestinal parasites that infect cats pose potential health threats for humans and animals. The present study is the first to report gastrointestinal (GIT) parasites in feces of stray cats from Gharbia governorate, Egypt. Findings were combined with those published in the earlier surveys from various Egyptian governorates, and various meta-analyses were conducted to underline the parasitic zoonoses from cats in Egypt.

Occurrence and molecular characterization of Acanthamoeba, Naegleria fowleri and Blastocystis in water samples from various sources in Egypt.

PCR-testing coupled to isolate sequencing was conducted to detect prevalence and various genotypes/subtypes of 3 neglected waterborne protists (Acanthamoeba, Naegleria fowleri and Blastocystis) in water samples from various sources in Dakahlia governorate, Egypt. Out of 62 protozoan-suspected samples by microscopy, Acanthamoeba was molecularly confirmed in 24 (38.7%) samples from various sources including tap water.

Molecular identification of different species infecting sheep and goats from Dakahlia governorate, Egypt.

Nematodes of genus can cause remarkable economic losses in the small ruminant`s industry and some species have a zoonotic potential. Since the most common source for human infection is the infected animals, accurate identification of different species in animals would reflect the species that can infect humans from the same area. The objective of the present study is to identify common species infecting small ruminants in Dakahlia governorate, Egypt using molecular-based techniques.

Combined Molecular and Lectin Binding Assays to Identify Different Trichostrongyle Eggs in Feces of Sheep and Goats from Egypt.

Background: Trichostrongyles are common causes of parasitic gastroenteritis in sheep and goats worldwide. Accurate identification of these nematodes to the genus and/or species level is important for therapy selection and control strategies. In the present study, molecular and egg-lectin binding approaches were employed to identify the most economically important trichostrongyles circulating in sheep and goat herds from six districts in Dakahlia governorate, Egypt.

Prevalence of species in sheep () from Dakahlia governorate, Egypt.

Feces from 184 sheep from Dakahlia governorate, Egypt were tested for species oocysts by using the standard floatation technique; oocysts were detected in 126 (68.4%). The prevalence was significantly higher in young sheep than adults.

First Evidence of Infection in Sheep from Egypt.

Trichostrongylid nematodes are a common cause of gastroenteritis in sheep. Despite its worldwide distribution, has not been included in reports listing the various trichostrongyles infecting sheep from Egypt. Herein, we describe the presence of 2 haplotypes infecting small ruminants from Egypt.

Molecular screening approach to identify protozoan and trichostrongylid parasites infecting one-humped camels (Camelus dromedarius).

Little is known about the diversity of many parasites infecting camels, with most relying on morphological parameters. DNA extracted from different tissues (n = 90) and fecal samples (n = 101) from dromedary camels (Camelus dromedarius) in Egypt were screened for multiple parasites using different molecular markers. Screening of tissue samples (heart) for Toxoplasma gondii and Sarcocystis spp.

Genetic variability within isolates of Sarcocystis species infecting sheep from Egypt.

In order to explain the exact role of sarcocystosis, principally Sarcocystis tenella, in losses of the Egyptian sheep industry, a precise confirmation about the existence of different Sarcocystis species infecting that economically important animal is needed. Therefore, this work aimed to molecularly identify, as well as illustrate the genetic variability within isolates of Sarcocystis spp. infecting sheep from Egypt.

Molecular identification of Trichodina compacta Van As and Basson, 1989 (Ciliophora: Peritrichia) from cultured Oreochromis niloticus in Egypt and its impact on immune responses and tissue pathology.

Trichodinids are peritrichous ciliated protozoa that affect both wild and cultured fishes. Several Trichodina species have low host specificity and are morphologically distinct, facilitating their identification based primarily on the presence of adhesive discs and the number of attached denticles. A trichodinid species named Trichodina compacta was first reported by Van As and Basson (1989) (Protozoa: Ciliophora: Peritrichia).