3'UTR enhances hCD47 cell surface expression, self-signal function, and reduces ER stress in porcine fibroblasts.

Introduction: Macrophages contribute to xenograft rejection by direct cytotoxicity and by amplifying T cell-mediated immune responses. It has been shown that transgenic expression of hCD47 protects porcine cells from human macrophages by restoring the CD47-SIRPα self-recognition signal. It has also been reported that the long 3' untranslated region (3'UTR) of the hCD47 gene, which is missing from constructs previously used to make hCD47 transgenic pigs, is critical for efficient cell surface expression in human cells.

Wide genetic variations at 18S ribosomal RNA locus of Cyclospora cayetanensis isolated from Egyptian patients using high resolution melting curve.

A variable clinical picture of cyclosporiasis including gastrointestinal tract (GIT) symptomatic or asymptomatic beside extraintestinal consequences suggests a possibility of heterogenicity of Cyclospora cayetanensis. The present work aimed to explore the possibility of genetic variation of C. cayetanensis using high-resolution melting (HRM) curve of polymerase chain reaction (PCR) amplified 18S rRNA genes.

Evaluation of circulating Transforming growth factor-beta1, Glypican-3 and Golgi protein-73 mRNAs expression as predictive markers for hepatocellular carcinoma in Egyptian patients.

Hepatocellular carcinoma (HCC) incidence is fast-growing especially in countries highly prevalent with viral hepatitis. Its poor prognosis has driven the research toward the discovery of sensitive markers for early detection. We investigated the usefulness of serum Transforming growth factor-beta1 (TGF-β1), Glypican-3 (GPC3), and Golgi protein-73 (GP73) mRNAs as early biomarkers in HCC Egyptian patients chronically infected with hepatitis C virus (HCV) in comparison with serum alpha-fetoprotein (AFP).

Helicobacter Pylori DNA in Liver Tissues From Chronic Hepatitis C Egyptian Patients.

Background: Hepatitis C virus (HCV) is considered the most common etiology of chronic liver disease in Egypt, which may progress to cirrhosis and hepatocellular carcinoma (HCC). Previous studies have documented an association between () infection and liver cirrhosis with or without HCC. This study aimed to investigate the presence of DNA in the liver tissue of Egyptian patients with chronic hepatitis C (CHC).

Serum interleukin-8 and insulin like growth factor-1 in Egyptian bladder cancer patients.

Background: Bladder cancer is a major health problem in Egypt as it represents the most common malignancy.

Aim: Evaluation of the potential usefulness of serum IL-8 and IGF-1 in Egyptian bladder cancer patients.

Methods: Serum levels of IL-8 and IGF-1 were determined in 51 bladder cancer patients and 45 controls using a chemiluminescence enzyme immunometric assay.

Alterations in neutrophil surface expression of L-selectin (CD62L) and soluble L-selectin (sL-selectin) in hemodialysis patients: relation to HCV.

High levels of L-selectin (CD62L) are a strong indicator of endothelial dysfunction, and atherosclerosis. Atherosclerosis is the leading cause of mortality and morbidity in hemodialysis (HD) patients. Whether HCV infection (highly prevalent in HD patients and also associated with alterations in adhesion molecules) would affect the leukocytic expression and/or the soluble form of L-selectin in hemodialysis patients is unknown.

Expression of telomerase reverse transcriptase in psoriatic lesional skin.

Background: Little information is known about telomerase expression in the chronic benign hyperproliferative skin disease, psoriasis. Further studies are still required to investigate its usefulness as a biomarker of this skin disorder.

Aim: To investigate the expression of human telomerase reverse transcriptase (hTERT) in psoriatic lesional skin and its relation to disease severity.

TT virus infection among Egyptian patients with hepatocellular carcinoma.

Hepatitis B and C viruses (HBV and HCV) have been associated with hepatocellular carcinoma (HCC). Recently, a novel DNA virus was isolated from a patient with posttransfusion hepatitis of unknown etiology and designated TT virus (TTV). To examine whether this virus is associated with HCC, we investigated sera from 82 Egyptian patients with histopathologically-diagnosed HCC.