Purification and characterization of latent polyphenol oxidase from truffles (Terfezia arenaria).

The polyphenol oxidase was extracted and purified from truffles (Terfezia arenaria) and it exhibited a molecular weight of 67 kDa. The truffle PPO was able to oxidize monophenolic, o-diphenolic and triphenolic substrates. Thus, the enzyme seems to be stable under wide range of pH and temperature.

Characterization of three polyphenol oxidase isoforms in royal dates and inhibition of its enzymatic browning reaction by indole-3-acetic acid.

In this study, Polyphenol oxidases (PPO) from royal dates fruit was purified and characterized. These procedures led to 8-fold purification with 14.72% recovery.

Antioxidant and antiradical properties of methanolic extracts from algerian wild edible desert truffles (terfezia and tirmania, ascomycetes).

Wild edible truffles (namely, Terfezia leonis, Tirmania pinoyi, and T. nivea) were commercially obtained from Southern Algeria and methanolic extracts were prepared from these truffles. Their antioxidant and antiradical properties were studied by using five analytical methods: scavenging capacity on 1,1-diphenyl-2-picrylhydrazyl (DPPH·), 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS·+), superoxide anion (O2·-) radicals, ferric reducing antioxidant power (FRAP), and ferricyanide/Prussian blue assay.