Utility of whole exome sequencing in the evaluation of isolated fetal growth restriction in normal chromosomal microarray analysis.

Objective: To investigate the application of whole exome sequencing (WES) in the prenatal diagnosis of isolated fetal growth restriction (FGR) with a normal result by chromosomal microarray analysis (CMA).

Methods: This retrospective study included singleton fetuses with isolated FGR in Guangdong Women and Children Hospital between July 2018 and August 2023. All fetuses were subjected to invasive prenatal testing with CMA and WES.

Superficial Temporal Artery-Middle Cerebral Artery Bypass Treatment Planning for Cerebral Ischaemia Based on Multi-Scale Model.

Superficial temporal artery and middle cerebral artery (STA-MCA) bypass surgery is an effective method to enhance cerebral blood flow (CBF) in ischemic patients. However, the effectiveness of various bypass techniques varies with the diversity of Circle of Willis (CoW) structures. This study aims to develop a physiologically realistic hemodynamic model to optimize STA-MCA bypass planning for cerebral ischemia patients with different CoW structures.

Association of Multiple Clefts Nuclei in Blasts with TCF3::PBX1 Fusion in B-Lymphoblastic Leukemia/Lymphoma.

Background: Several particular kinds of typical morphology characteristics of leukemic blasts associated with the specific subtypes of leukemia have been reported. However, B acute lymphoblastic leukemia/lymphoma (B-ALL/LBL) has rarely been reported. The purpose of this study was to investigate the correlation of TCF3::PBX1 fusion with multiple clefts nuclei of blasts in patients with B-ALL/LBL.

Modelling the effects of temperature, pH and osmotic shifts on the autofluorescence of in vitro.

Aims: This study aims to investigate how different wound microenvironmental factors (temperature, pH, and osmotic pressure) influence the autofluorescence of Staphylococcus aureus ( and its underlying molecular mechanisms, specifically focusing on the porphobilinogen synthase gene gene expression.

Methods: We measured the average fluorescence intensity of colonies under varying conditions of pH (3, 5, 7, 9, 11), temperature (25°C, 31°C, 37°C, 43°C), and osmotic pressure (0.9%, 1.

Tailoring Self-Catalytic N─Co Bonds into Heterostructure Architectures: Deciphering Polytellurides Conversion Mechanism Toward Ultralong-Lifespan Potassium Ion Storage.

Transition metal tellurides (TMTes) are promising anodes for potassium-ion batteries (PIBs) due to their high theoretical specific capacity and impressive electronic conductivity. Nevertheless, TMTes suffer from persistent capacity degradation due to the large volume expansion, high ion-diffusion energy barriers, and the dissolution/shuttle of potassium polytellurides (KTe). Herein, a heterostructured CoTe composite equipped with a self-catalytic center (N-CoTe/LTTC) is developed, exploiting its low-tortuosity tunneling, chemical tunability, and self-catalytic properties to elevate cycling stability to new heights.

Mesoporous Nanogel Sprays as Universal Evaporation Interface Modifiers for Boosting Water-Cluster Evaporation.

Accelerating water evaporation is vital for processes like photosynthesis, dehydration, and desalination. Optimizing the pore structure and interfacial properties of evaporative materials can reduce evaporation enthalpy and increase efficiency. However, integrating the evaporation interface with water transport channels poses significant design challenges and complicates low-enthalpy evaporation analysis.

Identification and functional characterization of T-cell exhaustion-associated lncRNA AL031775.1 in osteosarcoma: a novel therapeutic target.

Background: Osteosarcoma, an aggressive bone malignancy predominantly affecting children and adolescents, presents significant therapeutic challenges with a 5-year survival rate below 30% in metastatic cases. T-cell exhaustion, characterized by the overexpression of immune checkpoint molecules, contributes to osteosarcoma progression and immune evasion. Although targeting these inhibitory pathways has shown potential in restoring T-cell activity, the molecular regulators of T-cell depletion in osteosarcoma are poorly understood.

Integrated multiomics analysis and machine learning refine neutrophil extracellular trap-related molecular subtypes and prognostic models for acute myeloid leukemia.

Background: Neutrophil extracellular traps (NETs) play pivotal roles in various pathological processes. The formation of NETs is impaired in acute myeloid leukemia (AML), which can result in immunodeficiency and increased susceptibility to infection.

Methods: The gene set variation analysis (GSVA) algorithm was employed for the calculation of NET score, while the consensus clustering algorithm was utilized to identify molecular subtypes.

Bioinformatics analysis reveals novel tumor antigens and immune subtypes of skin cutaneous melanoma contributing to mRNA vaccine development.

Introduction: Skin cutaneous melanoma (SKCM) is a common malignant skin cancer with high mortality and recurrence rates. Although the mRNA vaccine is a promising strategy for cancer treatment, its application against SKCM remains confusing. In this study, we employed computational bioinformatics analysis to explore SKCM-associated antigens for an mRNA vaccine and suitable populations for vaccination.

Application of Ultrasound Localization Microscopy in Evaluating the Type 2 Diabetes Progression.

Type 2 diabetes is considered as a chronic inflammatory disease in which the dense microvasculature reorganizes with disease progression and is highly correlated with β cell mass and islet function. In this study, we constructed rat models of type 2 diabetes and used ultrasound localization microscopy (ULM) imaging to noninvasively map the pancreatic microvasculature at microscopy resolution in vivo to reflect β cell loss and islet function deterioration, and evaluate the efficacy after anti-cytokine immunotherapy. It was unveiled that ULM morphological and hemodynamic parameters have a strong link with β cell loss and deterioration of pancreatic islet function.

Cepharanthine hydrochloride: a novel ferroptosis-inducing agent for prostate cancer treatment.

Background: Ferroptosis is an intracellular iron-dependent cell death that is distinct from apoptosis, necrosis, and autophagy. Increasing evidence indicated that ferroptosis plays a crucial role in suppressing tumors, thus providing new opportunities for cancer therapy. The drug cepharanthine, commonly used to treat leukopenia, has been discovered to function as an anticancer agent to multiple types of cancer via diverse mechanisms.

Minocycline-Loaded Titanium Dioxide Nanoparticles for Augmented Synergistic Periodontal Sonodynamic Chemotherapy.

Background: The current clinical treatment of periodontitis usually involves mechanical removal of pathogenic bacteria through ultrasonic scaling and root planing, supplemented with antibacterial medications to inhibit microbial overgrowth. However, the therapeutic efficiency remains unsatisfactory due to complicated periodontal anatomy, limited plaque removal, short retention of antibiotics, and related side effects.

Methods And Results: To address these issues, we successfully synthesized mesoporous titanium dioxide nanoparticles (MTN) via a sol-gel method, which were modified with hemoglobin (Hb) and loaded with minocycline (MINO).

Bibliometric and visual analysis in the field of electroacupuncture's analgesia and regulation on negative emotion from 2014 to 2024.

Introduction: This bibliometric study systematically analyzes the research landscape of electroacupuncture (EA), focusing on its applications in pain relief and emotional regulation from 2014 to 2024. EA, a contemporary adaptation of traditional acupuncture, has gained significant attention for its potential therapeutic benefits in managing chronic pain and mood disorders.

Methods: Using the Web of Science Core Collection as the primary data source, we identified 537 articles related to EA's therapeutic effects.

Cortical Activation and Functional Connectivity Response to Different Interactive Modes of Virtual Reality (VR)-Induced Analgesia: A Prospective Functional Near-Infrared Spectroscopy (fNIRS) Study.

Aim: Virtual reality (VR) can be analgesic through intercortical modulation. This study investigated neural activities and correlates during different interactive modes.

Methods: Fifteen healthy participants (4M, 11F, age 21.

Changes in Respiratory Sensitivity Status of Patients in a Hospital in Shanxi Province Before and After the COVID-19 Epidemic.

Background: The lifting of the regional blockade in early December 2022 in Shanxi Province, China, caused an epidemic of Coronavirus disease 2019 (COVID-19). And the high allergy season from July to September each year.

Purpose: To investigate the effect of the COVID-19 epidemic on the respiratory sensitivity status of the population, to provide a scientific and effective basis for the prevention, diagnosis, condition assessment, and treatment of allergic respiratory diseases.

Mechanical countermeasures for spaceflight-associated neuro-ocular syndrome during 30-days of head down tilt bed rest: design, implementation, and tolerability.

After longer duration space missions, some astronauts experience structural and functional changes in the eye and structural changes in the brain, termed Spaceflight-Associated Neuro-Ocular Syndrome (SANS). Countermeasures against SANS are required to minimize potential operation impacts and negative long-term health consequences. Headward fluid shifts, which appear to promote SANS, provide a target for countermeasures.

Development of a colloidal gold-based immunochromatographic strip for rapid detection of .

Background: (APV1), the causal agent of betel palm yellow leaf disease (YLD), has caused significant yield losses and decreased product quality of betel nuts, posing a serious economic threat to local farmers. There is an urgent need for a convenient and reliable method for the rapid detection and surveillance of APV1.

Methods: The Capsid protein (CP) of APV1 was expressed in and purified as antigen to immunize BALB/c mice.

Autophagy: regulating the seesaw of bone-fat balance.

The interrelationship between bone and fat can be described as a seesaw in bone homeostasis, in which both osteogenesis and adipogenesis occur in a delicate balance. Osteoblasts and adipocytes share a common origin and play key roles in osteogenesis and adipogenesis. Bone-fat balance indicates osteogenesis and adipogenesis keeps a balance for concordant distribution of trabecular bone and bone marrow adipose tissue in bone, thereby leading to the balance between bone metabolism and lipid metabolism.

Tissue-Resident Memory CD8+ T Cells: Differentiation, Phenotypic Heterogeneity, Biological Function, Disease, and Therapy.

CD8+ tissue-resident memory T cells (TRM) are strategically located in peripheral tissues, enabling a rapid response to local infections, which is different from circulating memory CD8+ T cells. Their unique positioning makes them promising targets for vaccines designed to enhance protection at barrier sites and other organs. Recent studies have shown a correlation between CD8+ TRM cells and favorable clinical outcomes in various types of cancer, indicating their potential role in immune checkpoint blockade (ICB) therapies.

HRR as a predictor of lung health: insights from the NHANES database.

Background: Chronic respiratory diseases (CRPD) are a global health threat characterized by oxidative stress, systemic inflammation, hypoxemia, and respiratory distress. Inflammatory indicators such as hemoglobin-to-red blood cell distribution width ratio (HRR) have been explored in relation to diseases of the respiratory system, but the correlation between HRR and pulmonary function has not been established. As part of this study, a representative sample of the National Health and Nutrition Examination Survey (NHANES) respondents aged 40 or over was used to examine the correlation between HRR and pulmonary function indices.

Role of environmental pollutants-induced ferroptosis in pulmonary diseases.

Respiratory diseases rank among the foremost causes of mortality and disability globally, with long-term exposure to environmental pollutants playing a critical role in their onset and progression. Despite this, the underlying mechanisms and effective targeted treatments for these disorders remain poorly understood, highlighting an urgent need for focused research. Cell death, a programmed cellular response to external harmful stimuli, including ferroptosis-a recently identified form of iron-dependent programmed cell death-emerges as a pivotal process.

Genome-wide association study of salicylic acid provides genetic insights for tea plant selective breeding.

Salicylic acid (SA) is a phenolic phytohormone widely believed to regulate plant growth and stress response. Despite its significance, the genetic basis of SA-mediated resistance to biotic stressors in tea plants is little understood. Our study investigated the genetic diversity, population structure, and linkage disequilibrium (LD) patterns of 299 tea accessions using 79 560 high-quality single nucleotide polymorphisms (SNPs) obtained from genotyping-by-sequencing (GBS) data.

The long non-coding RNA MSTRG.32189-PcmiR399b- module regulates phosphate accumulation and disease resistance to in pear.

Pear ring rot disease () is a significant threat to the healthy development of the pear industry. Recent research has identified the functional role of long non-coding RNAs (lncRNAs) in various biological processes of plants. The role of lncRNAs in the pear defense response remains unknown.

The Glucagon-Like Peptide-1 (GLP-1) Receptor Agonist Liraglutide Regulates Sirtuin-1-Mediated Neutrophil Extracellular Traps to Improve Diabetes-Induced Bone Metabolism Imbalance.

Background: Diabetes mellitus (DM) is a chronic metabolic disorder that disrupts normal bone remodeling.

Objectives: This study aimed to investigate how the glucagon-like peptide-1 (GLP-1) receptor agonist liraglutide (LIR) addresses bone metabolism imbalances induced by type-II diabetes.

Methods: Type-II diabetic rat models were established through a single intraperitoneal injection of streptozotocin (STZ).