Publications by authors named "Mouhamad Oseikria"
Article Synopsis
- The study aimed to assess if Chlamydia abortus can adhere to the zona pellucida of bovine embryos, both intact and free from the zona pellucida, following in vitro infection.
- It involved incubating bovine embryos with the bacteria and then washing them according to International Embryo Transfer Society (IETS) guidelines to evaluate the effectiveness of the washing process.
- Results showed that C. abortus DNA was present in all tested embryo batches, with a higher bacterial load found in ZP-free embryos compared to ZP-intact ones, indicating that the washing protocol may not effectively remove the bacteria.
Background: Supplementation of bovine oocyte-cumulus complexes during in vitro maturation (IVM) with 1 μM of docosahexaenoic acid (DHA), C22:6 n-3 polyunsaturated fatty acid, was reported to improve in vitro embryo development. The objective of this paper was to decipher the mechanisms of DHA action.
Results: Transcriptomic analysis of 1 μM DHA-treated and control cumulus cells after 4 h IVM showed no significant difference in gene expression.
The positive effect of n-3 polyunsaturated fatty acids (FAs) on fertility in ruminants seems to be partly mediated through direct effects on the oocyte developmental potential. We aimed to investigate whether supplementation with physiological levels of docosahexaenoic acid (DHA, C22:6 n-3 polyunsaturated fatty acids) during IVM has an effect on oocyte maturation and in vitro embryo development in cattle. We reported that DHA (0, 1, 10, or 100 μM) had no effect on oocyte viability or maturation rate after 22-hour IVM.
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