Publications by authors named "Motoshi Narita"
Background: Focal embouchure dystonia (FED) is a type of task-specific dystonia affecting wind instrument players, and it frequently ends their professional careers. The results of the treatments of FED in the reported literature are disappointing.
Objective: We report 3 patients with FED who were brass instrument players.
Article Synopsis
- The study investigated the role of phosphodiesterase 4 (PDE4) in HMG human malignant melanoma cells, finding that PDE4 activity is present and can be inhibited by rolipram.
- Specific PDE4 inhibitors and forskolin were shown to increase intracellular cAMP levels, which then influenced cell growth.
- The research indicates that targeting PDE4 could potentially be a new therapeutic strategy for treating human malignant melanoma, as previous studies have not explored this positive regulation of cell growth through PDE4 inhibition.
Article Synopsis
- The HOSM-2 osteosarcoma cell line, derived from a human mandible, has been subcultured for 15 years, with no change in cell shape or doubling time, but notable changes in chromosomal numbers and structures across generations.
- Later generations of HOSM-2 exhibited tumorigenicity in nude mice, alongside a mutation in the p53 gene that resulted in a stop codon, indicating a potential link between genetic changes and cancer development.
- These findings suggest that HOSM-2 cells, which express osteoblast markers and differ from limb-derived osteosarcoma lines, could serve as a useful model for studying mandibular osteosarcoma and osteoblast behavior
Reverse transcription-polymerase chain reaction (RT-PCR) analysis revealed that HOSM-1 cells, an osteosarcoma cell line established from human mandible, expressed mRNA for osteoblastic markers, such as alkaline phosphatase, osteonectin, osteocalcin and parathyroid hormone receptor, thus exhibiting an osteoblastic phenotype. We have investigated a possible role of cyclic nucleotide phosphodiesterases (PDEs) in osteosarcoma cells. RT-PCR analysis revealed that HOSM-1 cells expressed mRNA for PDE4A, 4B and 4C.
View Article and Find Full Text PDFLittle is known concerning the expression, distribution and function of phosphodiesterase (PDE) 3s in malignant tumor cells, including human malignant melanoma HMG and osteosarcoma HOSM-1 cells. PDE3 activity was detected in homogenates of HMG cells; however, much less activity was found in HOSM-1 cells. In HMG cells, most of the PDE3 activity was in the particulate fraction.
View Article and Find Full Text PDF