First seroprevalence survey of bovine anaplasmosis: an emerging tick-borne disease in commercial livestock and dairy farms in Bangladesh.

Bovine anaplasmosis is an infectious, tick-borne disease caused by species, which is accountable for huge economic loss in dairy industry. This study was aimed to determine the seroprevalence of bovine anaplasmosis on randomly selected 61 commercial dairy farms in 3 intensive regions of Bangladesh. A total of 1472 sera were analysed using VMRD Antibody Test Kit cELISA v2 for the presence of -specific antibodies.

Violence against physicians working in public tertiary care hospital of Bangladesh: a facility-based cross-sectional study.

Background: Violence against physicians in the workplace is a prevalent global issue, and Bangladesh is no exception. Such violence significantly disrupts healthcare delivery and the attainment of universal health coverage. This study aimed to comprehensively evaluate the prevalence, nature and associated risk factors of workplace violence (WPV) against physicians in Bangladesh.

Liquid Biopsy Snapshots of Key Phosphoproteomic Pathways in Lung Cancer Patients for Diagnosis and Therapy Monitoring.

Phosphorylation is a post-translational modification in proteins that changes protein conformation and activity for regulating signal transduction pathways. This mechanism is frequently impaired in lung cancer, resulting in permanently active constitutive phosphorylation to initiate tumor growth and/or reactivate pathways in response to therapy. We developed a multiplexed phosphoprotein analyzer chip (MPAC) that enables rapid (detection time: 5 min) and sensitive (LOD: 2 pg/μL) detection of protein phosphorylation and presents phosphoproteomic profiling of major phosphorylation pathways in lung cancer.

Toward Precision Deposition of Conductive Charge-Transfer Complex Crystals Using Nanoelectrochemistry.

The lack of understanding for precise synthesis and assembly of nano-entities remains a major challenge for nanofabrication. Electrocrystallization of a charge-transfer complex (CTC), tetrathiafulvalene bromide (TTF)Br, is studied on micro/nanoelectrodes for precision deposition of functional materials. The study reveals new insights into the entire CTC electrocrystallization process from the initial nanocluster nucleation to the final elongated crystals with hollow ends grown from the working electrode to the neighboring receiving electrode.

Optimal control for COVID-19 pandemic with quarantine and antiviral therapy.

In the absence of a proper cure for the disease, the recent pandemic caused by COVID-19 has been focused on isolation strategies and government measures to control the disease, such as lockdown, media coverage, and improve public hygiene. Mathematical models can help when these intervention mechanisms find some optimal strategies for controlling the spread of such diseases. We propose a set of nonlinear dynamic systems with optimal strategy including practical measures to limit the spread of the virus and to diagnose and isolate infected people while maintaining consciousness for citizens.

Efficacy of limited antiviral treatment, testing, hospitalization, and social distancing for COVID-19 pandemic.

The control measures of a pandemic must be cautiously evaluated, especially when resources are "limited". A model of COVID-19 transmission dynamics is applied to assess the impact of antiviral treatment, testing, hospitalization, and social distancing. Under the assumption of "unlimited" resources, five control strategies involving social distancing, testing, hospitalization, and antiviral treatment are tested.

Liquid-Metal-Assisted Deposition and Patterning of Molybdenum Dioxide at Low Temperature.

Molybdenum dioxide (MoO), considering its near-metallic conductivity and surface plasmonic properties, is a great material for electronics, energy storage devices and biosensing. Yet to this day, room-temperature synthesis of large area MoO, which allows deposition on arbitrary substrates, has remained a challenge. Due to their reactive interfaces and specific solubility conditions, gallium-based liquid metal alloys offer unique opportunities for synthesizing materials that can meet these challenges.

Phosphoprotein Biosensors for Monitoring Pathological Protein Structural Changes.

Current biotechnological developments are driving a significant shift towards integrating proteomic analysis with landmark genomic, methylomic, and transcriptomic data to elucidate functional effects. For the majority of proteins, structure and function are closely intertwined. Post-translational protein modifications (e.

Reading Conformational Changes in Proteins with a New Colloidal-Based Interfacial Biosensing System.

Many biological events such as mutations or aberrant post-translational modifications can alter the conformation and/or folding stability of proteins and their subsequent biological function, which may trigger the onset of diseases like cancer. Evaluating protein folding is hence crucial for the diagnosis of these diseases. Yet, it is still challenging to detect changes in protein folding, especially if they are subtle, in a simple and highly sensitive manner with the current assays.

An exosomal- and interfacial-biosensing based strategy for remote monitoring of aberrantly phosphorylated proteins in lung cancer cells.

It is a well-known phenomenon that cancer cells release key biological information such as DNA, RNA or proteins into body fluids (e.g., blood, urine or saliva).

Interfacial nano-mixing in a miniaturised platform enables signal enhancement and in situ detection of cancer biomarkers.

Interfacial biosensing performs the detection of biomolecules at the bare-metal interface for disease diagnosis by comparing how biological species derived from patients and healthy individuals interact with bare metal surfaces. This technique retrieves clinicopathological information without complex surface functionalisation which is a major limitation of conventional techniques. However, it is still challenging to detect subtle molecular changes by interfacial biosensing, and the detection often requires prolonged sensing times due to the slow diffusion process of the biomolecules towards the sensor surface.

Detection of aberrant protein phosphorylation in cancer using direct gold-protein affinity interactions.

Protein phosphorylation is one of the most prominent post-translational mechanisms for protein regulation, which is frequently impaired in cancer. Through the covalent addition of phosphate groups to certain amino-acids, the interactions of former residues with nearby amino-acids are drastically altered, resulting in major changes of protein conformation that impacts its biological function. Herein, we report that these conformational changes can also disturb the protein's ability to interact with and adsorb onto bare gold surfaces.