Opuntia ficus-indica cladodes extract inhibits human neutrophil pro-inflammatory functions and protects rats from acetic acid-induced ulcerative colitis.

The increased production of reactive oxygen species (ROS) by human neutrophils can lead to oxidative imbalances and several diseases, such as inflammatory bowel disease (IBD). Opuntia ficus-indica (O. ficus-indica) is rich in bioactive substances with anti-inflammatory properties.

Anxiolytic and Antidepressant Effects of Ethanolic Extract in Scopolamine-Induced Amnesia in Zebrafish: Supported by Molecular Docking Investigation Targeting Monoamine Oxidase A.

Plants of the genus have been used in folk medicine for wound healing, alleviating liver, stomach, and rheumatism pains, and as cognitive enhancers, sedatives, antiseptics, tonics, and stimulants. The present work aimed to evaluate whether (Tt) administered for 15 days attenuated cognitive deficits and exhibited anxiolytic and antidepressant profiles in scopolamine-induced amnesia in zebrafish. Animals were randomly divided into six groups (eight animals per group): (1)-(3) Tt treatment groups (1, 3 and 6 mg/L), (4) control, (5) scopolamine (SCOP, 0.

The Effect of a -Based Formulation in Alleviating Cholinergic System Impairment and Scopolamine-Induced Memory Loss in Zebrafish (): Insights from Molecular Docking and In Vitro/In Vivo Approaches.

L. (Tt) has been recently gaining attention for its pharmacological value, including its neuroprotective activities. In this study, we explore the neuroprotective effects of a extract in a zebrafish () model of scopolamine (SCOP)-induced memory impairment and brain oxidative stress.

Preventive Anti-inflammatory Effects of Apocynin on Acetic Acid-Induced Colitis in Rats.

Ulcerative colitis is an inflammatory bowel disease with a complex aetiology characterised by abnormal immune responses and oxidative stress-induced tissue injury. Inflammatory cells play an important role in the progression of this pathology through the overproduction of reactive oxygen species (ROS) from various sources including the NADPH oxidases (NOXs). The aim of this study was to investigate the preventive effect of apocynin, a natural antioxidant molecule and a selective inhibitor of NOXs, on acetic acid (AA)-induced ulcerative colitis in rats.

Antibacterial Properties of Methanolic Leaf Extracts of L. against Gram-Positive and Gram-Negative Pathogenic Bacteria.

L., a family tree, is widely used in traditional folkloric medicine for its pharmaceutical properties. In the present study, we investigated the phytochemical composition of four methanolic leaf extracts of of various origins (Algeria and Tunisia) using high-performance liquid chromatography (HPLC).

Chemical Composition of (Mill.) Swingle Methanolic Leaf Extracts and Assessment of Their Antibacterial Activity through Oxidative Stress Induction.

The present study was conducted to investigate the chemical composition of (Mill.) Swingle methanolic leaf extracts from geographically distinct regions and to assess their antimicrobial properties along with their ability to induce oxidative stress. The HPLC-DAD analysis revealed the presence of phenolic acids and flavonoids including chlorogenic acid, gallic acid, synapic acid, p-coumaric acid, apigenin, hyperoside, isoamnétine-3-O-beta-D-glucotrioside, quercetin, and isoquercetin in various amounts depending on the origin of tested extracts.

Phytochemical profiling, antioxidant potential and protective effect of leaves extract of tunisian autochthonous accessions against acute CCl-injured hepatotoxicity in mice.

leaves (VVL) are agro-industrial waste. In the current study, the phytochemical profile of leaves extracts (VVLE) of two Tunisian autochthonous accessions was determined via LC-UV-ESI/MS, and their antioxidant and hepatoprotective properties were also assessed. Mice were pretreated orally with VVLE (7.

The NADPH Oxidase Inhibitors Apocynin and Diphenyleneiodonium Protect Rats from LPS-Induced Pulmonary Inflammation.

Inflammation is the body's response to insults, for instance, lung inflammation is generally caused by pathogens or by exposure to pollutants, irritants and toxins. This process involves many inflammatory cells such as epithelial cells, monocytes, macrophages and neutrophils. These cells produce and release inflammatory mediators such as pro-inflammatory cytokines, lipids and reactive oxygen species (ROS).

Sustainable Extraction, Chemical Profile, Cytotoxic and Antileishmanial Activities In-Vitro of Some Species Leaves Essential Oils.

Anti-leishmanial drugs extracted from natural sources have not been sufficiently explored in the literature. Until now, leishmaniasis treatments have been limited to synthetic and expensive drugs. This study investigated, for the first time, the anti-leishmanial efficacy of essential oils (EOs) from the leaves of species (, , and ).

Preventive and healing effect of high dosing grape seed flour on CKD patients of various stages and aetiologies.

Introduction: Chronic kidney disease (CKD) is a worldwide public health concern characterised by a progressive alteration of renal function, and obesity, diabetes and hypertension are major risk factors. Oxidative stress is commonly observed in CKD patients resulting from an imbalance between overproduction of reactive oxygen species (ROS) and impairment of defense mechanisms. Therefore, strategies that alleviate oxidative stress may have major clinical implications towards improving vascular health and reducing the burden of CKD.

Grape Seed Flour Extends Longevity by Improving Multi-Organ Dysfunction and Age-Associated Oxidative Stress and Inflammation in Healthy Rat.

According to the free-radical theory of aging, accumulation of reactive oxygen species (ROS) within mitochondria throughout life span leads to impairment of the main biological macromolecules as DNA, lipids, and proteins, which might be at the basis of premature aging. One way to test experimentally such a hypothesis consists in intervention studies using antioxidant nutrients aimed at limiting or inhibiting ROS production that should be able to reduce the aging rate and disease pathogenesis. Grape seed flour (GSF) contains a high level of phytochemicals among which bioactive polyphenols exhibit numerous biological properties and beneficial health effects as antioxidant, anti-inflammatory, anticarcinogenic, multi-organ (heart, liver, kidney, and brain among others) protective.

Untargeted Phytochemical Profile, Antioxidant Capacity and Enzyme Inhibitory Activity of Cultivated and Wild Lupin Seeds from Tunisia.

Lupin seeds can represent a valuable source of phenolics and other antioxidant compounds. In this work, a comprehensive analysis of the phytochemical profile was performed on seeds from three species, including one cultivar () and two wild accessions ( and ), collected from the northern region of Tunisia. Untargeted metabolomic profiling allowed to identify 249 compounds, with a great abundance of phenolics and alkaloids.

SSR markers and seed quality traits revealed genetic diversity in durum wheat (Triticum durum Desf.).

Genetic diversity and differences among durum-wheat cultivars evolved in various regions of the world are important for sustainable production in the current climate change scenario. Information regarding genetic differences was also important for the correct choice of parental material for the selection of high quality cultivars. Two elite and six obsolete cultivars of durum-wheat were characterized with 25-simple sequence repeats (SSR) markers.

Change of the litter fall, decomposition, and nutrient release in cork oak forest after anthropogenic disturbances in North West of Tunisia.

In Mediterranean forests, anthropogenic disturbances received little interest in regards to their shrub layer induced enlargement. We studied in the cork oak forest of Beni Métir and in undisturbed and disturbed sites, the relative contribution of the tree (L, DL) and shrub (L, DL) layers to litter fall, litter decomposition, and nutrients dynamic. Our results showed that disturbance significantly (p < 0.

Essential oils from species inhibit biofilm formation and the virulence of EPEC 2348/69.

Enteropathogenic E2346/69 (EPEC) has caused foodborne outbreaks worldwide and the bacterium forms antimicrobial-tolerant biofilms. The anti-biofilm formation of various components of essential oils extracted from selected medicinal plants were investigated and tested on EPEC and wild strains of . Oils extracted from the family Asteraceae and their major common constituents at 0.

Gastroprotective effect of leaf extract of two varieties grapevine ( L.) native wild and cultivar grown in North of Tunisia against the oxidative stress induced by ethanol in rats.

leaves are traditionally used in Tunisian folk medicine to treat digestive pathologies. We aimed to compare the gastroprotective effects of hydromethanolic leaves extracts of wild and cultivated grapes accessions native of Tunisia. The phytochemical analysis of grapevine leaves extracts was performed.

Increase interval training intensity improves plasma volume variations and aerobic performances in response to intermittent exercise.

Purpose: We studied the effect of two interval training programs of varying intensities (100% vs. 110% of maximal aerobic velocity [MAV]) on hematocrit (Ht), hemoglobin (Hb), and plasma volume variations (PVV) in young men.

Methods: Thirty-nine male volunteered were assigned to two control groups (CG, n = 9 and CG, n = 10), and two training groups (one with 100% MAV [EG, n = 10] and one with 110% MAV [EG, n = 10]).

Anticancer Activity of .

The extensive applications of species and their rich bioactive secondary metabolites have inspired many pharmacological investigations. Previous research has been conducted to examine the biological activities and numerous interesting pharmaceutical activities have been reported. However, the antitumor activities of these species are unclear.

Liver histopathology and biochemical biomarkers in Gobius niger and Zosterisessor ophiocephalus from polluted and non-polluted Tunisian lagoons (Southern Mediterranean Sea).

The aim of this study was to appraise the response of a multi-marker approach in fish species, Gobius niger and Zosterisessor ophiocephalus, in a polluted lagoon (Bizerte lagoon: MB and ML sites) and in a reference site (Ghar-El-Melh lagoon entrance) by the analysis of physiological indexes, liver histopathology and some biochemical biomarkers. The results showed liver hypertrophy in fish collected from Bizerte lagoon as well as many non-specific lesions, unlike the reference site. All Bizerte lagoon sites had the same prevalence of histopathological lesions, but the mean intensity (MI) of parasites seemed to be more sensible as an indicator of pollution levels.

Circadian variation in anticonvulsant activity of valproic acid in mice.

Purpose: This study aims to investigate whether valproic acid (VPA) anticonvulsant activity varied according to circadian dosing-time in mice.

Methods: VPA was administered to mice at four circadian stages (1, 7, 13 and 19h after light onset, (HALO)). The controls received a saline injection followed by a s.

Activity of anti-inflammatory, analgesic and antigenotoxic of the aqueous flower extracts of Opuntia microdasys Lem.Pfeiff.

Objectives: The aim of this study was to evaluate the analgesic and the anti-inflammatory activity of Opuntia microdasys at post flowering stage, F3 (OMF3) in rat and, in other hand, its antigenotoxic effects by the Allium cepa test.

Methods: OMF3 extracts were screened for activity of analgesic and anti-inflammatory using, respectively, the acetic acid writhing test in mice and the carrageenan-induced paw oedema assay in rats. The antigenotoxic has been evaluated by A.

Effects of the hydro-ethanolic extract of Marrubium vulgare in female rats.

Objective: To evaluate the effects of ethanol-water (80:20) extract of Marrubium vulgare (M. vulgare) on the hematological parameters, macroscopic and histological aspects of the uterus and fetus in non-pregnant and pregnant rats.

Methods: Female rats were divided into 4 equal groups (n = 9), group N (normal rats) and group G (pregnant rats) considered as control groups, group NE (normal rats treated with the ethanol-water (80:20, v/v) extract of M.

Dosing-time dependent oxidative effects of an immunosuppressive drug "Mycophenolate Mofetil" on rat kidneys.

This study investigates whether the toxicity in kidneys as well as oxidative stress varied according to the dosing time of an immunosuppressive agent "mycophenolate mofetil (MMF)" in Wistar Rat. 300mg/kg of MMF was injected by intraperitonal at four different circadian stages (1, 7, 13 and 19h after light onset, HALO). Rats were sacrificed after 3days, and the kidneys were removed for determination of oxidative stress and histological analysis.