[Epidemiological and immunological criteria of the effectiveness of immunization with adsorbed DPT vaccine by the old and new schedules].

The study revealed that the immunization of children with adsorbed DPT vaccine from the age of 3-4 months, as compared with the immunization of children from the age of 5-6 months, did not lead to an essential increase in the coverage of children with immunization at the period under study (1970-1983) and did not affect the total level of pertussis morbidity, as well as the proportion of children aged up to 1 year in the total number of pertussis cases. Children immunized at an early age produced antibodies in titers, equivalent to the titers in older children, but their immunity against pertussis, in contrast to their immunity against diphtheria and tetanus, was retained for a shorter period. The injection of adsorbed DPT vaccine at the age of 3-4 months was accompanied by a poorly pronounced increase in the content of IgG, the predominant synthesis of IgM and the suppression of the synthesis of IgA.

[Effect of normal and immune IgG, IgM and IgA on the intestinal microflora of mice with experimental dysbacteriosis].

Human IgG, IgM and IgA produce a pronounced protective effect, preventing enterobacteria from penetration into the mucous membrane of the proximal section of the small intestine of mice in antibiotic-induced dysbacteriosis. Normal mouse IgG and IgM, in contrast to IgA, are effective against mucosal enterobacteria of the small intestine. Immune mouse IgG, IgM and IgA show greater activity in protecting the mucous membrane than normal immunoglobulins of these classes.

[Comparative study of the immunoglobulins and specific antibodies in the blood of rabbits immunized with ADTP vaccine and ADT-anatoxin with normal and reduced antigen contents].

Adsorbed DPT vaccine and adsorbed DT toxoids with normal and reduced antigen content were used for the immunization of rabbits. The levels of IgM and IgG and the dynamics of antibodies to diphtheria and tetanus toxins and to Bordetella pertussis in the blood sera of the animals were studied in the postvaccinal period (on days 15 and 34). This study revealed that the reduction of the antigen content of adsorbed DT toxoid to 5 Lf of diphtheria toxoid and 5 binding units of tetanus toxoid did not decrease the capacity of the preparation for increasing the levels of IgG and IgM, antibodies to diphtheria and tetanus toxins in the sera of the rabbits.

[Sensitizing and protective activity of a staphylococcal vaccine made of water-soluble antigens, depending on the method and schedule of administration].

An experiment on guinea pigs immunized with staphylococcal vaccine prepared from water-soluble antigens revealed that the degree of developing sensitization and specific resistance was essentially determined by the method and schedule of the administration of the preparation. The intranasal administration of the vaccine induced a lesser degree of sensitization in comparison with its subcutaneous injection. The optimum response to the administration of the vaccine (a low sensitization level and a high degree of protection from infection) was observed in the animals immunized first intranasally and then by subcutaneous injection.

[Use of the pneumococcal serovar 3 erythrocyte antibody diagnostic agent for analyzing polysaccharide-containing preparations].

Antibody diagnosticum to pneumococci (serovar 3) has been prepared. The analysis of different antigenic preparations of pneumococci (serovars 1,3 and 6B) and the filtrates of culture fluid obtained in the process of the cultivation of bacteria belonging to serovars 3,9N and 23F has been carried out by means of the indirect hemagglutination test. The new diagnosticum has proved to be type-specific.

[The adjuvant effect of synthetic glycopeptides on humoral immune response to thymus-dependent antigen].

The comparative study of 5 analogs of muramyldipeptide (MDP) on primary and secondary immune response to bovine serum albumin has been studied. The adjuvant activity of the MDP analogs has been shown to be closely connected with their structure. Thus, the replacement of glutamine by aspartic acid leads to the loss of adjuvant properties, and the replacement of L-alanine by its stereoisomer causes a perceptible decrease in these properties.

[Relationship of the sensitizing and protective properties of pertussis antigens].

When comparing delayed hypersensitivity (DN) to B. pertussis corpuscular antigens with the agglutinogenic composition of B. pertussis, as well as to its histamine-sensitizing, leukocyte-sensitizing, adjuvant and hemagglutinating activity, no correlation was detected between the specific sensitizing properties of these antigens and the serotype and studied nonspecific properties of B.

[Effect of homosensitization on the immune response to ADPT vaccine].

The influence of homosensitization with syngeneic tissue antigens on immune response to adsorbed DPT vaccine and sheep red blood cells (SRBC) was studied in experiments in CBA mice and noninbred white mice. These experiments revealed that preliminary homosensitization suppressed the production of antibodies to the components of adsorbed DPT vaccine, while after immunization with SRBC the immunosuppressing effect of homosensitization was absent.

[Erythrocyte diagnostica made from salmonellal phagolysates].

For the first time O antigens obtained from phagolysates were proved to be suitable for use as material for the production of highly specific erythrocyte diagnostic preparations. O antigens obtained from Salmonella by two methods, i.e.

[Use of the passive hemagglutination inhibition reaction for the purpose of determining the antigenic activity of anatoxins in vitro].

The work deals with the study of the possibility of using the passive hemagglutination inhibition test (antibody neutralization) for the determination of the antigenic activity of botulinum toxoids, types A, B and E. Erythrocytic diagnostic preparations were shown to allow the determination of up to 0.1 g of antigen.

[Antigenic activity of Bordetella pertussis during cultivation in liquid nutrient media].

The authors studied the antigenic activity of Bordetella pertussis during stab cultivation. For the first time there were revealed changes (oscillations) of antigenic activity: formation of 3 peaks of which the middle was the highest. A possibility of improving the quality of pertussis vaccine by using biomass at the height of the peak was experimentally founded.

Obtaining hyperimmune anti-coli (anti-Escherichia) human plasma.

Anticoli (anti-Escherichia) plasma has been obtained from donors immunized with viable Escherichia oral vaccines, prepared from Str-d mutants of E. coli. Anticoli plasma has an therapeutic effect against diseases of Escherichia etiology.

[Immune response of mice of different inbred lines to Clostridium oedematiens anatoxin].

Mice belonging to a number of inbred strains were immunized intradermally with Cl. oedematiens alpha-toxoid. The immunization was repeated 30 days later.

[Use of the passive hemagglutination test for the purpose of determining antibody levels following animal immunization with Cl. perfringens toxoid].

Data are presented on the study of possibilities of the application of the passive hemagglutination test for titration of the blood sera of mice, guinea pigs and rabbits immunized with Cl. perfringens toxoid. A diagnostic agent obtained by the sensitization of formalin- and tannin-treated sheep erythrocytes with the serologically pure toxoid, and homologous sera (as standard) were used in this test.

[Reactivity, immunogenicity and epidemiological effectiveness of vaccination according to various schemes of immunization against whooping-cough, diphtheria and tetanus].

An extended controlled epidemiological trial was carried out for the purpose of studying the reactogenic properties, immunological and epidemiological efficacy of immunization against whooping cough, diphtheria and tetanus according to a scheme suggested by the authors (AKdeltaC-AKdeltaC-KB) in comparison with the official scheme (AKdeltaC-AKdeltaC-AKdeltaC). There was revealed some increase in the frequency of general reactions in children vaccinated by the experimental scheme; however strong general reactions and local reactions of different intensity were encountered with equal frequency in both groups. Two months after the end of the vaccination significantly higher titres of pertussis agglutinins were revealed in children immunized by the AKdeltaC-AKdeltaC-KB scheme; no significant difference was found in the content of the protective titres of diphtheria and tetanus antitoxins.