Roadmap on Label-Free Super-Resolution Imaging.

Label-free super-resolution (LFSR) imaging relies on light-scattering processes in nanoscale objects without a need for fluorescent (FL) staining required in super-resolved FL microscopy. The objectives of this Roadmap are to present a comprehensive vision of the developments, the state-of-the-art in this field, and to discuss the resolution boundaries and hurdles which need to be overcome to break the classical diffraction limit of the LFSR imaging. The scope of this Roadmap spans from the advanced interference detection techniques, where the diffraction-limited lateral resolution is combined with unsurpassed axial and temporal resolution, to techniques with true lateral super-resolution capability which are based on understanding resolution as an information science problem, on using novel structured illumination, near-field scanning, and nonlinear optics approaches, and on designing superlenses based on nanoplasmonics, metamaterials, transformation optics, and microsphere-assisted approaches.

Laser Beam Self-Focusing in Silicon at an Absorbed Wavelength by a Vortex Beam in the Same Wavelength.

In this research, we present a novel approach to achieving super-resolution in silicon using the plasma dispersion effect (PDE) that temporarily controls the complex refractive index of matter. By employing a laser vortex pump beam, which is absorbed in the silicon, we can shape the complex refractive index as a gradient index (GRIN) lens, enabling the focusing of a laser probe beam within the material. Our study introduces a single beam at a wavelength of 775 nm for both the pump and the probe beams, offering tunable focusing capabilities and the potential to attain higher spatial resolution.

Dynamics of laser-induced tunable focusing in silicon.

We report here on focusing of a probe IR (λ = 1.55 μm) laser beam in silicon. The focusing is done by a second pump laser beam, at λ = 0.

An Engineered Nanocomplex with Photodynamic and Photothermal Synergistic Properties for Cancer Treatment.

Photodynamic therapy (PDT) and photothermal therapy (PTT) are promising therapeutic methods for cancer treatment; however, as single modality therapies, either PDT or PTT is still limited in its success rate. A dual application of both PDT and PTT, in a combined protocol, has gained immense interest. In this study, gold nanoparticles (AuNPs) were conjugated with a PDT agent, meso-tetrahydroxyphenylchlorin (mTHPC) photosensitizer, designed as nanotherapeutic agents that can activate a dual photodynamic/photothermal therapy in SH-SY5Y human neuroblastoma cells.

Innovative functional polymerization of pyrrole-N-propionic acid onto WS nanotubes using cerium-doped maghemite nanoparticles for photothermal therapy.

Tungsten disulfide nanotubes (WS-NTs) were found to be very active for photothermal therapy. However, their lack of stability in aqueous solutions inhibits their use in many applications, especially in biomedicine. Few attempts were made to chemically functionalize the surface of the NTs to improve their dispersability.

Author Correction: Generation and Manipulation of Superoscillatory Hotspots Using Virtual Fourier Filtering and CTF Shaping.

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

Author Correction: Non-Invasive Imaging Through Scattering Medium by Using a Reverse Response Wavefront Shaping Technique.

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

Generation and Manipulation of Superoscillatory Hotspots Using Virtual Fourier Filtering and CTF Shaping.

Superoscillation is a technique that is used to produce a spot of light (known as 'hotspot') which is smaller than the conventional diffraction limit of a lens and even smaller than the optical wavelength. Over the past few years, several techniques have been realized for the generation of the superoscillatory hotspot. In this article, for the first time to the best of our knowledge, we propose a novel and a more efficient technique for producing superoscillation in microscopic imaging by shaping the Coherent Transfer Function (CTF) of a lens via virtual Fourier filtering followed by a phase retrieval algorithm.

Plasma dispersion effect based super-resolved imaging in silicon.

We present here a new method for shaping a pulsed IR (λ = 1550nm) laser beam in silicon. The shaping is based on the plasma dispersion effect (PDE). The shaping is done by a second pulsed pump laser beam at 532nm (in either a Gaussian mode or a donut mode) which simultaneously and collinearly illuminates the silicon's surface with the IR beam.

Improved Margins Detection of Regions Enriched with Gold Nanoparticles inside Biological Phantom.

Utilizing the surface plasmon resonance (SPR) effect of gold nanoparticles (GNPs) enables their use as contrast agents in a variety of biomedical applications for diagnostics and treatment. These applications use both the very strong scattering and absorption properties of the GNPs due to their SPR effects. Most imaging methods use the light-scattering properties of the GNPs.

Towards real-time detection of tumor margins using photothermal imaging of immune-targeted gold nanoparticles.

Background: One of the critical problems in cancer management is local recurrence of disease. Between 20% and 30% of patients who undergo tumor resection surgery require reoperation due to incomplete excision. Currently, there are no validated methods for intraoperative tumor margin detection.