Passive immunisation against Pseudomonas aeruginosa recombinant flagellin in an experimental model of burn wound sepsis.

Background: This study was aimed to investigate whether anti-recombinant flagellin type A (anti r-fla-A) immunoglobulin G (IgG) provides protection in a mouse burn model of infection, and to determine the role of anti r-fla-A IgG as an opsonin and motility inhibitor in local and systemic infections.

Methods: Following the preparation of r-flagellin type A, rabbit polyclonal IgG was prepared. Specificity of anti r-flagellin for r-flagellin was evaluated by immunoblot analysis.

The Essential Oils Activity of Eucalyptus polycarpa, E. largiflorence, E. malliodora and E. camaldulensis on Staphylococcus aureus.

Because of its resistance to antibiotics, Staphylococcus aureus causes many of problems in hospital and society. As one of the main reasons of clinical infections it can cause to serious surgical and cutaneous infections and pneumonia. The inhibitory effect of the essential oils include; Eucalyptus largiflorence, E.

The phenotypic and genotypic characterization of Bacillus anthracis isolates from Iran.

To understand epidemiology of Bacillus anthracis in Iran, the morphological, biochemical, and virulence specifications of 32 B. anthracis isolates, collected from human, sheep, cattle, goat, and environmental specimens obtained from throughout Iran were examined by conventional and molecular approaches. B.

Effect of cumin (Cuminum cyminum) seed essential oil on biofilm formation and plasmid Integrity of Klebsiella pneumoniae.

Seeds of the cumin plant (Cuminum cyminum L.) have been used since many years in Iranian traditional medicine. We assessed the effect of cumin seed essential oil on the biofilm-forming ability of Klebsiella pneumoniae strains and on the integrity of a native resistance plasmid DNA from K.

Modulatory effect of Acetobacter xylinum cellulose on peritoneal macrophages.

Introduction: The present work aimed to assess the effect of bacterial cellulose (BC) from Acetobacter xylinum on some functions of peritoneal macrophages.

Materials And Methods: Mice peritoneal macrophages were lavaged and co-cultured with various concentrations of BC in microtiter plate. Viability of macrophages was determined by MTT assay.

Prevalence of the genes encoding aminoglycoside-modifying enzymes and methicillin resistance among clinical isolates of Staphylococcus aureus in Tehran, Iran.

Aminoglycosides are potent bactericidal agents that play an important role in antistapylococcal therapy. In this study, we used a multiplex polymerase chain reaction assay to investigate the prevalence of aac(6')-Ie/aph(2''), ant(4')-Ia, and aph(3')-IIIa, the genes encoding the most clinically prevalent aminoglycoside-modifying enzymes, and simultaneous detection of the methicillin resistance gene, mecA, in Staphylococcus aureus isolates. A total of 100 S.

Cloning, expression, purification, and characterization of recombinant flagellin isolated from Pseudomonas aeruginosa.

Pseudomonas aeruginosa as an opportunistic pathogen causes lethal infections in immunocompromised individuals. This bacterium possesses a polar flagellum made up of flagellin subunits. Flagella have important roles in motility, chemotaxis, and establishment of P.

Effect of subinhibitory concentrations of cumin (Cuminum cyminum L.) seed essential oil and alcoholic extract on the morphology, capsule expression and urease activity of Klebsiella pneumoniae.

Cuminum cyminum L., commonly known as cumin, is a plant with a considerable reputation. The aim of this work was to study the activity of cumin seed essential oil and alcoholic extract against Klebsiella pneumoniae ATCC 13883 and clinical K.

In vivo induction of necrosis in mice fibrosarcoma via intravenous injection of type B staphylococcal enterotoxin.

The bacterial superantigen staphylococcal enterotoxin B (SEB) is a potent inducer of cytotoxic T-cell activity and cytokine production in vivo. We investigated the possibility of the therapeutic application of SEB in patients with fibrosarcoma. The anti-tumor effect of SEB in mice with inoculated fibrosarcoma (WEHI-164) was examined by intravenous (IV) and intratumoral (IT) injection and the sizes of the inoculated tumors, IFN-gamma production, and CD4+/CD8+ T cell infiltration were determined.

The antimicrobial effect of benzalkonium chloride on some pathogenic microbes observed on fibers of acrylic carpet.

In the presented research, the main aim is to investigate the antibacterial effectiveness of BAC solutions on acrylic fibers used in machinery carpet. An acrylic pile carpet laid in a public place for 30 days and the existence of some microbes were investigated on it. The antimicrobial effect of different BAC solutions for identified microbes was studied in vitro.