Metabolic and genes expression analyses involved in proline metabolism of two rose species under drought stress.

An investigation was conducted to assess proline anabolism and catabolism pathway genes under drought stress. Treatments were irrigation in three levels (25, 50 and 100% field capacity) at 1, 3, 6 and 12 d and two rose species (Rosa canina L. and Rosa damascene Mill.

Regulation of stomatal aperture in response to drought stress mediating with polyamines, nitric oxide synthase and hydrogen peroxide in L.

To assess the role of genes involved in polyamines synthesis, nitric oxide synthase (NOS), copper amine oxidase activity (CuAO) and hydrogen peroxide (HO) in regulation of stomatal aperture to drought stress in L., a study was performed at three irrigating levels (25%, 50%, and 100% field capacity) with three replications at 1, 3, 6 and 12 days. The results showed that putrescine (Put) accumulation occurred under both 50% and 25% FC at 1 d.

Role of genes and metabolites involved in polyamines synthesis pathways and nitric oxide synthase in stomatal closure on Rosa damascena Mill. under drought stress.

In order to evaluate the genes involved in polyamines synthesis pathway and the role of nitric oxide synthase (NOS) and HO in stomatal closure under drought stress, a research conducted with three irrigation levels (100, 50 and 25% field capacity) at 1, 3, 6 and 12 days on Rosa damascena Mill. HPLC and qPCR results showed that putrescine (Put) accumulation occurred at first day in both 50 and 25% of field capacity and then decreased the other days. Furthermore, Put accumulation in the indirect pathway (ADC, AIH and CPA) was more effective related to the direct pathway (ODC) under severe stress.

Applying the AOGCM-AR5 models to the assessments of land suitability for walnut cultivation in response to climate change: A case study of Iran.

Due to higher temperatures and lower water availability, climate change is likely to have a major impact on walnut production in the near future. Climate change will alter the land suitability for walnut cultivation around the world, especially in arid and semi-arid regions like Iran. Here, land suitability for the cultivation of walnut (Juglans regia L.

Isopentenyl Transferase (IPT) Gene Transfer to Perennial Ryegrass Through Sonication-Assisted Agrobacterium-Mediated Transformation (SAAT), Vacuum and Heat Treatment.

The successful introduction of isopentenyl transferase (IPT) gene into perennial ryegrass, cultivars Numan and Grassland using Agrobacterium tumefaciens via three explants (callus, seed and meristem tip) under three individual experiment was evaluated. In the first experiment, the calli were inoculated with LBA4404 Agrobacterium strain under vacuum, heat and in combination of both at 42 °C for 5 min followed by vacuum treatment (390 mm Hg pressure) for 15 min. Sonication-assisted Agrobacterium-mediated transformation (SAAT) was applied for seed and meristem tip transformation of perennial ryegrass for the first time.

Temporal characterization of 2-phenylethanol in strongly and weakly scented genotypes of damask rose.

The molecular and physiological properties of 2-phenylethanol (2-PE) in the strongly scented genotype (SSG) and a weakly scented genotype (WSG) of damask rose at six floral developmental stages were investigated. The chemical compositions of volatile emissions were determined by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) analysis of the floral headspace. In both genotypes, the relative percentage of 2-PE increased more in SSG than WSG, as flowers developed.

Application of ISSR markers to analyze molecular relationships in Iranian jasmine (Jasminum spp.) accessions.

There are many species of jasmines in different regions of Iran in natural or cultivated form, and there is no information about their genetic status. Therefore, inter-simple sequence repeat (ISSR) analysis was used to evaluate genetic variations of the 53 accessions representing eight species of Jasminum collected from different regions of Iran. A total of 21 ISSR primers were used which generated 981 bands of different sizes.

Seismomorphogenesis: a novel approach to acclimatization of tissue culture regenerated plants.

Plantlets under in vitro conditions transferred to ex vivo conditions are exposed to biotic and abiotic stresses. Furthermore, in vitro regenerated plants are typically frail and sometimes difficult to handle subsequently increasing their risk to damage and disease; hence acclimatization of these plantlets is the most important step in tissue culture techniques. An experiment was conducted under in vitro conditions to study the effects of shaking duration (twice daily at 6:00 a.

Micropropagation of Araucaria excelsa R. Br. var. glauca Carrière from orthotropic stem explants.

The objectives of the present work were in vitro propagation of Araucaria excelsa R. Br. var.

RAPD fingerprint to appraise the genetic fidelity of in vitro propagated Araucaria excelsa R. Br. var. glauca plantlets.

Randomly amplified polymorphic DNA (RAPD) was used as a tool to assess the genetic fidelity of in vitro propagated Araucaria excelsa R. Br. var.

Microsatellite analysis of Damask rose (Rosa damascena Mill.) accessions from various regions in Iran reveals multiple genotypes.

Background: Damask roses (Rosa damascena Mill.) are mainly used for essential oil production. Previous studies have indicated that all production material in Bulgaria and Turkey consists of only one genotype.