Publications by authors named "Morteza Hosseini"

The challenge of rapid identification of bacteria in food and water still persists as a major health problem. To tackle this matter, we have developed a single-probe liquid crystal (LC)-based optical sensing platform for the differentiation of five common bacterial strains, including , , , , and , using cysteine-functionalized silver nanotriangles as signal enhancers. Unique optical patterns were generated from the interaction of the samples with the LC interface and captured by using a camera under polarized light.

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Article Synopsis
  • An advanced electrochemiluminescence (ECL) detection system has been developed, combining a bipolar electrode system with MXene material for improved electrical conductivity and redox-active surface area.
  • The system utilizes the ROR1 antibody to enhance ECL signals through an immunoassay with a modified Ag triangle, enabling sensitive cancer detection.
  • The low-cost immunosensors demonstrated high sensitivity over a wide range (10 fg/mL to 1 µg/mL) and achieved approximately 90% accuracy in diagnosing real cancer samples.
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Quantum dot-based optical biosensors represent a significant advancement for detection of mycotoxins that are toxic secondary metabolites produced by fungi and pose serious health risk effects. This review highlights the importance of detection of filamentous fungi such as Aspergillus, Penicillium, Fusarium, Claviceps, and Alternaria in mycotoxin production, leading to contamination of agricultural products and subsequent health issues. Conventional detection methods such as thin-layer chromatography, high-performance liquid chromatography, gas chromatography, and enzyme-linked immunosorbent assay are discussed with their respective advantages and limitations.

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The rapid and accurate detection of pathogens is crucial for effective disease prevention and management in healthcare, food safety, and environmental monitoring. While conventional pathogen detection methods like culture-based techniques and PCR are sensitive and selective, they are often time-consuming, require skilled operators, and are not suitable for point-of-care or on-site testing. To address these limitations, innovative sensor technologies have emerged that leverage the unique properties of nanomaterials.

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Electrochemiluminescence (ECL) sensing systems have surged in popularity in recent years, making significant strides in sensing and biosensing applications. The realization of high-throughput ECL sensors hinges on the implementation of novel signal amplification strategies, propelling the field toward a new era of ultrasensitive analysis. A key strategy for developing advanced ECL sensors and biosensors involves utilizing novel structures with remarkable properties.

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Nano-based drug delivery systems are regarded as a promising tool for efficient epilepsy treatment and seizure medication with the least general side effects and socioeconomic challenges. In the current study, we have designed a smart nanoscale drug delivery platform and applied it in the kindling model of epilepsy that is triggered rapidly by epileptic discharges and releases anticonvulsant drugs in situ, such as carbamazepine (CBZ). The CBZ-loaded electroactive ferrocene nanoliposomes had an average diameter of 100.

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Using a chemiluminescence reaction between luminol and HO in basic solution, an ultrasensitive electrochemiluminescence (ECL) aptasensor was developed for the determination of tobramycin (TOB), as an aminoglycoside antibiotic. TiC/Ni/Sm-LDH-based nanocomposite effectively catalyzes the oxidation of luminol and decomposition of HO, leading to the formation of different reactive oxygen species (ROSs), thus amplifying the ECL signal intensity of luminol, which can be used for the determination of TOB concentration. To evaluate the performance of the electrochemiluminescence aptasensor and synthesized nanocomposite, different methods such as cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) analyses were performed.

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A method is presented for chemiluminescence resonance energy transfer (CRET) using APTES-FeO as a highly efficient energy acceptor with strong magnetic effectiveness over extended distances, while an Au@BSA-luminol composite acts as the donor. In order to boost the chemiluminescence reactions, CuO nanoparticles were successfully employed. The distance between the donor and acceptor is a crucial factor in the occurrence of the CRET phenomenon.

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To address the need for facile, rapid detection of pathogens in water supplies, a fluorescent sensing array platform based on antibiotic-stabilized metal nanoclusters was developed for the multiplex detection of pathogens. Using five common antibiotics, eight different nanoclusters (NCs) were synthesized including ampicillin stabilized copper NCs, cefepime stabilized gold and copper NCs, kanamycin stabilized gold and copper NCs, lysozyme stabilized gold NCs, and vancomycin stabilized gold/silver and copper NCs. Based on the different interaction of each NC with the bacteria strains, unique patterns were generated.

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We have developed a rapid, facile liquid crystal (LC)-based aptasensor for E. coli detection in water and juice samples. A textile grid-anchored LC platform was used with specific aptamers adsorbed via a cationic surfactant, cetyltrimethylammonium bromide (CTAB), on the LC surface.

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MicroRNAs (miRNAs) are associated with certain types of cancer, tumor stages, and responses to treatment, thus efficient methods are required to identify them quickly and accurately. Abnormal expression of microRNA-191 (miR-191) has been linked to particular cancers and several other health conditions, such as diabetes and Alzheimer's disease. In this study, a new dual-biosensor based on the zirconium and preasodium-based metal-organic framework (Zr/Pr MOF) was developed for the rapid, ultrasensitive, and selective detection of miRNA-191.

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Early detection of the coronavirus is acknowledged as a crucial measure to mitigate the spread of the pandemic, facilitating timely isolation of infected individuals, and disrupting the transmission chain. In this study, we leveraged the properties of synthesized Ag-MOF, including high porosity and increased flow intensity. Electrochemical techniques such as cyclic voltammetry (CV) and differential pulse voltammetry (DPV) were employed to develop an economical and portable sensor with exceptional selectivity for COVID-19 detection.

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An innovative electrochemical sensor is introduced that utilizes bipolar electrochemistry on a paper substrate for detecting glucose in sweat. The sensor employs a three-dimensional porous nanocomposite (MXene/NiSm-LDH) formed by decorating nickel-samarium nanoparticles with double-layer MXene hydroxide. These specially designed electrodes exhibit exceptional electrocatalytic activity during glucose oxidation.

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Graphitic-phase carbon nitride (g-CN) materials have exhibited increasingly remarkable performance as emerging electrochemiluminescence (ECL) emitters, owing to their unique optical and electronic properties; however, the ECL merits of porous g-CN nanofibers doped with ternary metals are not yet explored. Deciphering the ECL properties of trimetal-doped g-CN nanofibers could provide an exquisite pathway for ultrasensitive sensing and imaging with impressive advantages of minimal background signal, great sensitivity, and durability. Herein, we rationally synthesized g-CN nanofibers doped atomically with Mn, Fe, and Co elements (Mn/Fe/Co/g-CN) in a one-pot via the protonation in ethanol and annealing process driven by the rolling up mechanism.

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A fast and sensitive aptasensor was developed using nanoplates with peroxidase activity as a novel approach detection is described using a silver/platinum nanoplate (Ag/Pt NPL) that interacts with an oligonucleotide aptamer as a bioreceptor. The size of the Ag/Pt NPLs was about 42 nm according to the FE-SEM images. The EDS result indicates that a thin layer of Pt ions was coated on the surface of the Ag NPLs.

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This study introduces a chitosan/boehmite biocomposite as an efficient adsorbent for removing anionic Congo Red (CR) and non-ionic Bromothymol Blue (BTB) from water. Boehmite nanoparticles were synthesized using the Sol-gel method and then attached to chitosan particles using sodium tripolyphosphate through co-precipitation method. Characterized through FTIR, FE-SEM, BET, and XRD, the biosorbent displayed structural integrity with optimized pH conditions of 3 for CR and 4 for BTB, achieving over 90 % adsorption within 30 min.

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Urea is a common milk adulterant that falsely increases its protein content. Excessive consumption of urea is harmful to the kidney, liver, and gastrointestinal system. The conventional methods for urea detection in milk are time-consuming, costly, and require highly skilled operators.

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Lactate is a metabolite that holds significant importance in human healthcare, biotechnology, and the food industry. The need for lactate monitoring has led to the development of various devices for measuring lactate concentration. Traditional laboratory methods, which involve extracting blood samples through invasive techniques such as needles, are costly, time-consuming, and require in-person sampling.

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FeO@SiO@NiAl-LDH three-components microsphere contains a Fe3O4@SiO2 magnetic core and a layered double hydroxide with nickel cation provide the binding ability to (His)-tagged-protein and exhibits high performance in protein separation and purification. The morphology and chemistry of the synthesized FeO@SiO@NiAl-LDH microspheres were characterized by energy-dispersive X-ray spectroscopy (EDX), scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transform infrared (FTIR), vibrating sample magnetometer (VSM), Dynamic light scattering (DLS). Purified enzyme was assesed with SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis and intrinsic fluorescence spectroscopy.

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Point-of-care testing plays a crucial role in diagnostics within resource-poor areas, necessitating the utilization of portable and user-friendly devices. The adaptation of biosensors for point-of-care applications requires careful considerations, such as miniaturization, cost-effectiveness, and streamlined sample processing. In recent years, the electrochemiluminescence (ECL) immunoassay has gained significant attention due to its visual detection capabilities and ability to facilitate high-throughput analysis.

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A dual-targeting nanobiosensor has been developed for the simultaneous detection of AMELX and AMELY genes based on the different fluorescence signals emitted from gold and silver nanoclusters, AuNCs and AgNCs respectively. In our design, both catalytic hairpin assembly (CHA) and hybridization chain reaction (HCR) have been used as isothermal, enzyme-free and simple methods for signal's amplification. The working principle is based on the initiation of a cascade of CHA-HCR reactions when AMELX is present, in which AuNCs, synthesized on the third hairpin, are aggregated on the surface of the dsDNA product, performing the phenomenon of aggregation induced emission (AIE) and enhancing their fluorescence signal.

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In this study, an aptamer-based, functionalized-DNA hydrogel system is developed for prostate-specific antigen (PSA) detection. A pure DNA hydrogel is constructed using specific DNA building blocks and an aptamer as a cross-linker. Firstly, silver nanoclusters (AgNCs) are constructed on the Y-shaped DNA (Y-DNA) building blocks.

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Phenylalanine dehydrogenase (PheDH) has been proposed as an ideal protein scaffold for the one-step and green synthesis of highly efficient multifunctional gold nanoclusters. The PheDH-stabilized fluorescent gold nanoclusters (PheDH-AuNCs) with dual emission/single excitation exhibited excellent and long-term stability, high water solubility, large Stokes shift and intense photoluminescence. Selectivity studies demonstrated that the red fluorescence emission intensity of PheDH-AuNCs was obviously decreased in less than 10 min by the addition of mercury, copper, cysteine or glutathione under the single excitation at 360 nm, without significant change in the blue emission of the PheDH-AuNCs.

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Corona Virus Disease 2019 (COVID-19) as the infectious disease caused the pandemic disease around the world through infection by SARS-CoV-2 virus. The common diagnosis approach is Quantitative RT-PCR (qRT-PCR) which is time consuming and labor intensive. In the present study a novel colorimetric aptasensor was developed based on intrinsic catalytic activity of chitosan film embedded with ZnO/CNT (ChF/ZnO/CNT) on 3,3',5,5'-tetramethylbenzidine (TMB) substrate.

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Terpenes are a widespread class of natural products with significant chemical and biological diversity, and many of these molecules have already made their way into medicines. In this work, we employ a data science-based approach to identify, compile, and characterize the diversity of terpenes currently known in a systematic way, in a total of 59,833 molecules. We also employed several methods for the purpose of classifying terpene subclasses using their physicochemical descriptors.

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