Ultrasound-Assisted Extraction, LC-MS/MS Analysis, Anticholinesterase, and Antioxidant Activities of Valuable Natural Metabolites from Willd.: In Silico Molecular Docking and In Vitro Enzymatic Studies.

The Willd. plant's phenolic constituent extraction and identification were optimized using the ultrasound-assisted extraction (UAE) method and the LC-MS/MS analysis, respectively. Additionally, cupric reducing antioxidant capacity (CUPRAC), beta carotene, reducing power, DMSO alcalin, silver nanoparticle (SNP)-based method, phenanthroline, and hydroxyl radical tests were utilized to assess the extract's antioxidant capacity, while the neuroprotective effect was examined in vitro against acetylcholinesterase enzyme.

Optimization of Supercritical Carbon Dioxide Extraction of Oil and Its Antimicrobial, Antioxidant, and Anticancer Activities.

is a medicinal plant with different bioactive compounds that have an essential role in biomedicine applications, especially in Arab nations. However, traditional extraction methods for oils can lead to the loss of some volatile and non-volatile oils. Therefore, this study aimed to optimize the supercritical fluid extraction (SFE) of oils from at pressures (10, 20, and 48 MPa).

Identification of peptides, metal binding and lipid peroxidation activities of HPLC fractions of hydrolyzed oat bran proteins.

The aim of this study was to evaluate metal binding and antioxidant activities of hydrolyzed oat bran proteins followed by the determination of peptide sequences. Protamex oat bran protein hydrolysates (OBPH) were separated by reverse-phase HPLC into eight peptide fractions (F1-F8) and their abilities to either chelate metals (Fe, Ca) or prevent the oxidation of lipids were investigated. In the Fe chelation assay, OBPH had significantly ( < 0.

Reduction of hexavalent chromium by digested oat bran proteins.

Hexavalent chromium (Cr(VI)) is associated with an increase in oxidative stress, such as DNA, lipid and protein oxidations. This study investigated, for the first time, the ability of digested food proteins to reduce Cr(VI) and scavenge free radicals. Protamex, an endopeptidase was used to digest oat bran protein isolates for 1, 2, 3, and 4h.