An improved high cell density cultivation-iHCDC-strategy for leucine auxotrophic K12 ER2507.

Fed-batch processes are commonly used tools in high cell density cultivation of . However, the setup of a fed-batch process can be challenging, especially when working with genetically modified strains. This work deals with the design of a specific strategy for the leucine auxotrophic strain K12 ER2507 that is of interest for arabinose-inducible gene expression systems due to its mutation.