Generation of -Triflylimino-λ-iodanes: Application to Imidation of Phosphines and Catalytic α-Amidation of 1,3-Dicarbonyl Compounds.

We describe the imidation of phosphines and α-amidation of 1,3-dicarbonyl compounds using -triflylimino-λ-iodane, which is generated from iodosylarene and triflylamide without any other additives. Furthermore, the imino-λ-iodane catalytically generated from an iodoarene precatalyst with oxone and triflylamide promotes α-amidation of 1,3-dicarbonyl compounds, representing the first method catalyzed by imino-λ-iodane.

A practical fluorogenic substrate for high-throughput screening of glutathione S-transferase inhibitors.

We report a new fluorogenic substrate for glutathione S-transferase (GST), 3,4-DNADCF, enabling the assay with a low level of nonenzymatic background reaction. Inhibitors against Noppera-bo/GSTe14 from Drosophila melanogaster were identified by high throughput screening using 3,4-DNADCF, demonstrating the utility of this substrate.

Preparation of bis(diazo) compounds incorporated into butadiyne and thiophene units and generation and characterization of their photoproducts.

(2,6-dimethyl-4-tert-butylphenyl)(2,4,6-tribromophenyl)diazomethane(-N(2)) was found to be stable enough to survive under Sonogashira coupling reaction conditions, and aryldiazomethyl substituents were introduced at the 1,4-positions of butadiyne (4-2N(2)) and the 2,5-positions of thiophene(5-2N(2)). Irradiation of those bis(diazo) compounds generated bis(carbenes), which were characterized by using ESR and UV/vis spectroscopic techniques in a matrix at low temperature as well as time-resolved UV/vis spectroscopy in solution at room temperature. These studies revealed that both of the bis(carbenes), 4 and 5, have singlet quinoidal diradical ground states with a very small singlet-triplet energy gap of less than 1 kcal mol(-1).

Preparation of bis(diazo) compounds incorporated into butadiyne and thiophene units, and generation and characterization of bis(carbene) from these compounds.

[9-[10-(4-t-Butyl-2,6-dimethyl)phenyl]anthryl](4-bromo-2,6-dimethylphenyl)diazomethane (1-N(2)) was found to be stable enough to survive under Sonogashira and Suzuki coupling reaction conditions, and bis(diazo) compounds incorporated into the 1,4-positions of butadiyne (3-2N(2)) and the 2,5-position of thiophene (4-2N(2)) were prepared. Irradiation of those bis(diazo) compounds generated bis(carbenes), which were characterized by ESR and UV-vis spectroscopic techniques in a matrix at low temperature, as well as time-resolved UV-vis spectroscopy in solution at room temperature. These studies revealed that both of the bis(carbenes), 3 and 4, have a singlet quinoidal diradical ground state with a very small singlet-triplet energy gap of less than 1 kcal/mol.

[A case report of anterior mediastinal cystic lymphangioma].

Fourty seven years old woman came to our hospital for further examination of incidentally found abnormal chest shadow. Chest US examination revealed lobulated cystic mass. The cyst wall was thin and smooth.

Immunosuppressive mechanism of 15-deoxyspergualin on sinusoidal lining cells in swine liver transplantation: suppression of MHC class II antigens and interleukin-1 production.

To elucidate the precise mechanism of action of 15-deoxyspergualin (DSG) in swine liver transplantation, the expression of MHC class II antigens (Ia) on hepatic sinusoidal lining cells (SLC) and their production of interleukin-1 (IL-1) were examined. In our previous study, we isolated sinusoidal endothelial cells (SEC) and Kupffer cells (KC) by enzymatic digestion and centrifugal elutriation, and demonstrated that both SEC and KC present alloantigens effectively and generated IL-1 in response to allogenic or lipopolysaccharide stimulation. Animals were divided into three groups: group 1, nontransplanted normal controls (n = 3); group 2, no immunosuppressive treatment following liver transplantation (n = 5); group 3, DSG (0.

Induction of antiidiotypic antibodies by donor-specific blood transfusions. Establishment of a human-mouse hybridoma secreting the MLR-inhibiting factor.

We describe a patient transfused with 200 ml of donor fresh whole blood three times at 2-week intervals. Three weeks after the last transfusion, transplantation and splenectomy were done at the same time. Splenic cells from this DST pretreated patient were fused with murine myeloma cells (X63-Ag8, 653).