AFNOR validation of Premi Test, a microbiological-based screening tube-test for the detection of antimicrobial residues in animal muscle tissue.

Premi Test contains viable spores of a strain of Bacillus stearothermophilus which is sensitive to antimicrobial residues, such as beta-lactams, tetracyclines, macrolides and sulphonamides. The growth of the strain is inhibited by the presence of antimicrobial residues in muscle tissue samples. Premi Test was validated according to AFNOR rules (French Association for Normalisation).

Elimination of aminoglycoside antibiotics in milk following intramammary administration.

The elimination in cow milk of aminoglycoside antibiotic residues (neomycin, dihydrostreptomycin, kanamycin and gentamicin) was studied after intramammary administration of eight drug formulations marketed in France. Quantitative residue analysis was performed by a cylinder plate method. The sensitivity was 0.

Residues of macrolide antibiotics in eggs following medication of laying hens.

1. The elimination kinetics of four macrolide antibiotics (tylosin, erythromycin, spiramycin and josamycin) in eggs were determined separately for albumen and yolk after oral administration through either drinking water or diet or after intramuscular injection. 2.

Sulphonamide residues in milk of dairy cows following intravenous injection.

An investigation was conducted into the elimination into milk of four sulphonamides (sulphadimidine, sulphamethoxypyridazine, sulphadimethoxine and sulphadoxine) after intravenous injections of drugs available in France. Sulphonamide determination in milk was performed using a high-performance liquid chromatography (HPLC) method with detection limit of 0.01 micrograms/ml.

Excretion of penicillins and cephalexin in bovine milk following intramammary administration.

The elimination into bovine milk of beta-lactam antibiotic residues (procaine penicillin G, cloxacillin, ampicillin, oxacillin, cephalexin) following intramammary administration of 10 preparations marketed in France was studied. The quantitative analysis of residues was carried out by a microbiological agar diffusion method using Bacillus stearothermophilus. Sensitivity ranged from 0.

Excretion of tetracycline and chlortetracycline in eggs after oral medication of laying hens.

After dosing laying hens orally with tetracycline (TC) through either drinking water (0.25 and 0.5 g/l for 5 days) or feed (300 and 600 ppm for 7 days), and chlortetracycline (CTC) through feed (600 ppm) residues were determined by an agar plate diffusion technique in cylinders with Bacillus cereus as test-organism, separately for albumen and for yolk.

Excretion of oxytetracycline in eggs after medication of laying hens.

The kinetics of oxytetracycline elimination into eggs were determined separately for albumen and yolk after oral administration through either drinking water (0.1-0.25 and 0.

[Elimination of polypeptide antibiotics (colistin and bacitracin) in the milk after intramuscular or intramammary administration].

The elimination in cow's milk of polypeptidic antibiotic residues (colistin sulfate, colistin methanesulfonate and bacitracin) was investigated following intramuscular and intramammary administrations of eight drugs marketed in France. The quantitative analysis was performed according to the microbiological method of agar diffusion. The mean elimination periods lasted between three and six milkings for injected preparations, between four and six milkings for intramammary ones.

Excretion of penicillins in bovine milk following intramuscular administration.

The kinetics of elimination into milk of sodium penicillin G, procaine penicillin G, benzathine penicillin G, ampicillin and amoxycillin residues have been determined after intramuscular administration of eleven drugs chosen among those commercially available in France. These investigations will be used as a basis to estimate and harmonize the withdrawal times demanded for veterinary drugs. The quantitative analysis of residues was carried out by a cylinder plate microbiological method with Bacillus stearothermophilus as test organism.