The Effect of Window Size on Pathologists' Search for Rare Elements in a Digital Pathology Setting.

Context.—: Digital pathology requires pathologists to assess tissue digitally rather than on an analog microscope, which has been the mainstay tool for tissue assessment for more than a century. The impact of different digital interaction configurations on pathologists' performance is not well understood.

Sellar and perisellar meningiomas: effects on pituitary function in a Spanish cohort observational study.

Purpose: Studies focused on the effects of sellar and/or perisellar (S/PS) meningiomas on pituitary function are scarce. The primary objective of the present study was to determinate the effects that S/PS meningiomas and their treatments have on pituitary function. Also, we described the clinical characteristics and therapeutic outcomes of the cohort of adult Spanish patients.

[Pediatric guidelines for coeliac disease: Treatment and follow-up].

The goal of the treatment is to relieve symptoms, achieve duodenal mucosal healing, avoid long term complications, and ensure children´s appropriate growth, for which it´s necessary to follow a lifelong, nutritionally complete and healthy gluten free diet (GFD). The Celiac Disease Working Group of the Gastroenterology Committee of the Sociedad Argentina de Pediatría developed this guide based on expert consensus, aimed at gastroenterologists, pediatricians and primary care physicians with the objective of updating the following topics: Treatment. Definition of gluten free food.

3D-Printable Rotary Valves for Low-Pressure Pneumatic and Fluidic Applications in Analytical Chemistry.

We report on a fused deposition modeling 3D-printable rotary valve fabricated from high-grade plastics such as polyether ether ketone or lower-grade plastics like polylactic acid. The valve weighs less than 90 g and has the potential to be integrated into portable and autonomous chemical analysis systems. It has been demonstrated to be leak-proof up to 2.

Partial biochemical response of adrenal artery embolization for pheochromocytoma: A case report and review of the literature.

We present the case of a 22-year-old male with a left adrenal pheochromocytoma, initially diagnosed during a workup for thoracic pain. The patient's tumor was refractory to medical management, and surgical resection was ruled out due to high cardiovascular risk, stemming from cyanotic congenital heart disease, aortic aneurysm, and factor VII deficiency. The patient underwent adrenal artery embolization (AAE) as a salvage treatment.

Comparing cryoprecipitate-poor plasma to fresh frozen plasma as replacement therapy in thrombotic thrombocytopenic purpura: An updated meta-analysis.

Background: Cryoprecipitate-poor plasma (CPP) has been suggested as a promising alternative to the standard fresh frozen plasma (FFP) in plasma exchange therapy (TPE) for thrombotic thrombocytopenic purpura (TTP) given its lower concentrations of von Willebrand Factor (VWF). However, its efficacy and safety remain a topic of debate.

Study Design And Methods: We conducted a systematic review and meta-analysis comparing CPP to FFP during TPE in patients with TTP.

Prevalence of hybrid TLR4M2 monocytes/macrophages in peripheral blood and lung of systemic sclerosis patients with interstitial lung disease.

Introduction: Systemic sclerosis (SSc) is a complex autoimmune connective tissue disease characterized by microvascular damage, immune system reactivity and progressive fibrosis of skin and internal organs. Interstitial lung disease is the leading cause of death for SSc patients (SSc-ILD), and the process of lung fibrosis involves also circulating monocytes and alveolar macrophages.

Methods: Current study aimed to identify monocyte/macrophage phenotypes in lung and peripheral blood of SSc-ILD patients by immunostaining and flow cytometry, respectively.

Influence of Sodium Borohydride Content on Triangular Silver Nanoprisms Dropped on Copper Hydroxide Nanowire-Based SERS Substrates.

In this work, surface-enhanced Raman scattering substrates with triangular silver nanoprisms (AgNPrs) dropped on copper hydroxide nanowires (CuOHNWs) were evaluated. AgNPrs were synthesized in colloidal solution using Ag nitrate, polyvinylpyrrolidone, trisodium citrate dihydrate, hydrogen peroxide, and sodium borohydride (NaBH). A set of five solutions with volume percentages from 0.

Histological ovarian features and hormonal determinations in assigned females at birth transgender individuals according to different testosterone preparations.

Introduction: Distinct androgen formulations have been used as gender-affirming hormone treatment, but little is known about the specific changes that may occur in the ovary depending on the testosterone preparation used. The study aims to evaluate the histological modifications of the ovarian tissue and the hormonal changes after gender-affirming surgery based on the testosterone preparation employed, such as testosterone cypionate or undecanoate.

Design: Unicenter transversal cohort study.

Fatty Acid Analysis by Capillary Electrophoresis and Contactless Conductivity Detection for Future Life Detection Missions.

Future life-detection missions will likely search for biosignatures within a wide range of organic compounds, including fatty acids. In order to determine such biosignatures, it is necessary to identify and quantify individual fatty acids present within a sample. In this study, we present a method using capillary electrophoresis coupled to contactless conductivity detection (CE-CD) for the separation and detection of both saturated and unsaturated fatty acids after derivatization with N,N-diethylethylenediamine, triethylamine, and 2-chloro-1-methylpyridinium iodide at 40°C for 10 min.

[Pediatric coeliac disease: Clinical features and diagnostic process].

Celiac disease is a chronic condition that affects approximately 1% of the world's population, becoming a public health problem. The numerous publications and international guides published in recent years make it necessary to update aspects related to its diagnosis. Highly sensitive and specific serological determinations have allowed the identification of the clinical polymorphism.

Anti-LGI1 encephalitis and Langerhans cell histiocytosis: Two associated entities? A case report.

Autoimmune encephalitis (AE) is an immune-mediated condition that induces brain inflammation due to several neural-specific autoantibodies. The main triggering and predisposing factors are infections, genetics, the use of immune checkpoint inhibitors and tumors. We report a case of a 57-year-old male with a biopsy-confirmed Langerhans cell histiocytosis (LCH) and a concomitant anti-LGI1 encephalitis discussing a possible relationship in the pathogenesis of these phenomena.

Liquid Organic Hydrogen Carrier Concepts and Catalysts for Hydrogenation and Dehydrogenation Reactions.

Background: The issue of renewable energy (RE) source intermittency, such as wind and solar, along with the geographically uneven distribution of the global RE potential, makes it imperative to establish an energy transport medium to balance the energy demand and supply areas. A promising energy vector to address this situation is hydrogen, which is considered a clean energy carrier for various mobile and portable applications. Unfortunately, at standard pressure and temperature, its energy content per volume is very low (0.

Unravelling the mechanisms underlying the social interaction effects on the feed efficiency of rabbits.

The feed consumption and feeding patterns of group-housed livestock and their productive outputs can be affected by interactions among group members, such as competition for feeder access and aggression. The impact of competition is especially significant when feed restriction is implemented during the fattening stage, a common practice in rabbit farms to reduce mortality from digestive disorders during this period. The overall aim was to quantify the importance of social interaction effects at the genetic and environmental level on feed efficiency, feeding behaviour and social ranking traits in rabbit populations having access to electronic feeders whilst they were housed in groups during fattening.

Biomarkers of atrial fibrillation-related pathways and left atrial structure and function in an overweight and obese population.

Background: Exploring longitudinal associations of blood biomarkers with left atrial (LA) structure and function can enhance our understanding of atrial fibrillation (AF) etiopathogenesis.

Methods: We studied 532 participants of the PREDIMED-Plus trial, a multicenter randomized trial in overweight and obese adults with metabolic syndrome. At baseline, 3 and 5 years after randomization, participants underwent transthoracic echocardiography and provided blood for serum biomarker measurements [propeptide of procollagen type I (PICP), high-sensitivity (hs) troponin T (hsTnT), hs C-reactive protein (hsCRP), 3-nitrotyrosine (3-NT), and N-terminal propeptide of B-type natriuretic peptide (NT-proBNP)].

Feeder cell training shapes the phenotype and function of in vitro expanded natural killer cells.

Natural killer (NK) cells are candidates for adoptive cell therapy, and the protocols for their activation and expansion profoundly influence their function and fate. The complexity of NK cell origin and feeder cell cues impacts the heterogeneity of expanded NK (eNK) cells. To explore this, we compared the phenotype and function of peripheral blood-derived NK (PB-NK) and umbilical cord blood-derived NK (UCB-NK) cells activated by common feeder cell lines, including K562, PLH, and 221.

Genome-wide fitness analysis of reveals mutants are attenuated due to iron restriction .

is a globally disseminated pathogen that is the cause of over 100 million infections per year. The resulting diseases are dependent upon host susceptibility and the infecting serovar. As serovar Typhimurium induces a typhoid-like disease in mice, this model has been used extensively to illuminate various aspects of infection and host responses.

RANK in cancer-associated fibroblasts: A valuable prognostic determinant for metastasis in early-stage breast cancer patients.

Background: The molecular system of receptor activator of nuclear factor kappa-β (RANK) and its ligand (RANKL) plays a role in a variety of physiological and pathological processes. These encompass the regulation of bone metabolism, mammary gland development, immune function, as well as their involvement and tumorigenesis. Nevertheless, limited knowledge exists regarding their function within the tumor microenvironment.

Front-line liquid biopsy for early molecular assessment and treatment of hospitalized lung cancer patients.

Background: Molecular characterization is pivotal for managing non-small cell lung cancer (NSCLC), although this process is often time-consuming and patients' conditions might worsen while molecular analyses are processed. Our primary aim was to evaluate the performance of "up-front" next-generation sequencing (NGS) through liquid biopsy (LB) of hospitalized patients with newly detected lung neoplasm in parallel with conventional diagnosis. The secondary aim included longitudinal monitoring through LB of patients with oncogenic alterations at baseline.

Blood Parameters in Children and Adolescents With Underweight and Atypical Anorexia Nervosa.

Objective: This study aimed to identify biochemical, hematological, and endocrinological abnormalities in a sample of children and adolescents with underweight AN and atypical AN and to compare these results between the two groups.

Method: Based on the 5th BMI-percentile admission, adolescents with underweight AN (n = 520) and atypical AN (n = 255) were included and medical records were reviewed.

Results: Low prealbumin (35%) and neutropenia (39%), and several abnormalities in endocrinological parameters (50%) were the most common alterations found in the whole sample.

Novel truncating germline variant reinforces as a susceptibility gene for familial non-medullary thyroid cancer.

Background: It has long been observed that there are families in which non-medullary thyroid cancer (NMTC) occurs, but few syndromes and genes have been described to date. Proteins in the shelterin complex have been implied in cancer. Here, we have studied shelterin genes in families affected by NMTC (FNMTC).

Underground speciation: Unraveling the systematics and evolution of the highly diverse tuco-tucos (genus Ctenomys) with genomic data.

Subterranean rodents of the genus Ctenomys (tuco-tucos) are endemic to South America and have experienced relatively recent radiation. There are about 67 recognized species that originated in approximately 1-2 MY. They stand out for their species richness, extraordinary chromosomal diversity, and wide range of habitat they occupy in the continent.