A novel strategy for the quantify of emerging isomeric pollutants belonging to the dihydroxybenzene family for environmental sample monitoring by amperometric detection.

This study introduces an innovative approach for quantifying isomeric pollutants utilizing an amperometric sensor. The determination of the isomers hydroquinone and catechol is based on the use of a glassy carbon electrode modified with Cu@PtPd/C nanoparticles (Cu@PtPd/C/GCE) in core-shell form, showing significant electrocatalytic activity in the oxidation of the later compounds. The determination was carried out at two different potentials: one at which where only hydroquinone is oxidized, and another in which where both hydroquinone and catechol are oxidized.

Minimum variance optimized Fisher ratio analysis of comprehensive two-dimensional gas chromatography / mass spectrometry data: Study of the pacu fish metabolome.

Integration of rice and fish farming, eg., pacu fish in Argentina, has raised concern that herbicides used for rice paddies may adversely affect the fish metabolome. To study this issue, tile-based Fisher ratio (F-ratio) analysis was applied to comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometry (GC × GC-TOFMS) data of pacu fish raised in an integrated rice-fish farming system (farmed class) versus fish raised in tanks (tank-raised class) to discover class-distinguishing analytes.

Molecular epidemiological insights into Trypanosoma vivax in Argentina: From the endemic Gran Chaco to outbreaks in the Pampas.

Argentina is a home to millions of beef and dairy cattle and is one of the world's major exporters of meat. In the present study, Trypanosoma vivax was prevalent (2016-2018) in two major livestock farming regions, the Gran Chaco and the Pampas. In the Gran Chaco, 29% and 51% of animals (n = 72, taurine x zebuine crossbreed) were, respectively, positive by TviCATL-PCR and the more sensitive fluorescent fragment length barcoding (FFLB) method.

Delayed sleep timing and circadian rhythms in pregnancy and transdiagnostic symptoms associated with postpartum depression.

Later sleep timing, circadian preference, and circadian rhythm timing predict worse outcomes across multiple domains, including mood disorders, substance use, impulse control, and cognitive function. Disturbed sleep is common among pregnant and postpartum women. We examined whether sleep timing during third trimester of pregnancy predicted postpartum symptoms of mania, depression, and obsessive-compulsive disorder (OCD).

Heme oxygenase-1 arrests Leydig cells functions and impairs their regulation by histamine.

Testicular Leydig cells (LC) are modulated by several pathways, one of them being the histaminergic system. Heme oxygenase-1 (HO-1), whose upregulation comprises the primary response to oxidative noxae, has a central homeostatic role and might dysregulate LC functions when induced. In this report, we aimed to determine how hemin, an HO-1 inducer, affects LC proliferative capacity and whether HO-1 effects on LC functions are reversible.

Natural Frequencies of seiches in Lake Chapala.

This research presents an analysis of the frequencies and vibration modes of the structure of the superficial seiches of Lake Chapala using mathematical modelling and measurements. The HAMSOM model was run with wind fields from coastal meteorological stations and for different lake storage levels. The lake water level measurements were carried out in two campaigns in 2003.

Claudin-19 mediates the effects of NO on the paracellular pathway in thick ascending limbs.

Claudins are a family of tight junction proteins that provide size and charge selectivity to solutes traversing the paracellular space. Thick ascending limbs (TALs) express numerous claudins, including claudin-19. Nitric oxide (NO), via cGMP, reduces dilution potentials in perfused TALs, a measure of paracellular permeability, but the role of claudin-19 is unknown.

Thick Ascending Limb Sodium Transport in the Pathogenesis of Hypertension.

The thick ascending limb plays a key role in maintaining water and electrolyte balance. The importance of this segment in regulating blood pressure is evidenced by the effect of loop diuretics or local genetic defects on this parameter. Hormones and factors produced by thick ascending limbs have both autocrine and paracrine effects, which can extend prohypertensive signaling to other structures of the nephron.

Structure-Performance Relationships of Temperature-Responsive PLGA-PEG-PLGA Gels for Sustained Release of Bone Morphogenetic Protein-2.

PLGA (poly(lactic-co-glycolic) acid)-PEG (polyethylene glycol)-PLGA synthesis conditions have an impact on the physicochemical features of the copolymer and its usefulness as biomaterial. This study reports on an analysis of the composition and structural properties of PLGA-PEG-PLGA copolymers applying a variety of analytical techniques. Viscoelastic properties and particularly the temperature-responsive behavior of PLGA-PEG-PLGA showed a marked dependence on copolymer structural features.

Nitric oxide reduces paracellular resistance in rat thick ascending limbs by increasing Na and Cl permeabilities.

About 50% of the Na reabsorbed in thick ascending limbs traverses the paracellular pathway. Nitric oxide (NO) reduces the permselectivity of this pathway via cGMP, but its effects on absolute Na ([Formula: see text]) and Cl ([Formula: see text]) permeabilities are unknown. To address this, we measured the effect of l-arginine (0.

Development of an ultra high performance liquid chromatography method for determining triamcinolone acetonide in hydrogels using the design of experiments/design space strategy in combination with process capability index.

An ultra high performance liquid chromatography method was developed and validated for the quantitation of triamcinolone acetonide in an injectable ophthalmic hydrogel to determine the contribution of analytical method error in the content uniformity measurement. During the development phase, the design of experiments/design space strategy was used. For this, the free R-program was used as a commercial software alternative, a fast efficient tool for data analysis.

[Epidemiology of caprine and ovine brucellosis in Formosa province, Argentina].

An epidemiological study of brucellosis was carried out in 516 goats and mixed flocks (goat/sheep) from the three agro-ecological regions of Formosa province, Argentina. Serum samples from a total of 25401 goats and 2453 sheeps were analyzed using buffered plate agglutination test (BPAT) and complement fixation test (CFT). Bacteriological and PCR analyses on milk samples from goats in three flocks with a history of brucellosis and recent abortions were also performed.

Nitric oxide decreases the permselectivity of the paracellular pathway in thick ascending limbs.

Thick ascending limbs reabsorb 25% to 30% of the filtered NaCl. About 50% to 70% is reabsorbed via the transcellular pathway and 30% to 50% is reabsorbed through the Na-selective paracellular pathway. Nitric oxide (NO) inhibits transepithelial Na reabsorption, but its effects on the paracellular pathway are unknown.

Histamine inhibits adrenocortical cell proliferation but does not affect steroidogenesis.

Histamine (HA) is a neurotransmitter synthesized in most mammalian tissues exclusively by histidine decarboxylase enzyme. Among the plethora of actions mediated by HA, the modulatory effects on steroidogenesis and proliferation in Leydig cells (LCs) have been described recently. To determine whether the effects on LCs reported could be extrapolated to all steroidogenic systems, in this study, we assessed the effect of this amine on adrenal proliferation and steroidogenesis, using two adrenocortical cell lines as experimental models, murine Y1 cells and human NCI-H295R cells.

[Odynophagia, fever and rash in a 33 year-old woman].

Hand-Foot-Mouth disease is a viral exanthematous disease primarily caused by Coxsackie virus that mainly affects children under 10 years-old during the spring or summer. It is a rare disease in adults, and rarer still in the immunocompetent. We report the case of a 33-year-old immunocompetent adult affected bys Hand Foot Mouth disease.

Proliferative effect of histamine on MA-10 Leydig tumor cells mediated through HRH2 activation, transient elevation in cAMP production, and increased extracellular signal-regulated kinase phosphorylation levels.

Mast cells (MC) occur normally in the testis with a species-specific distribution, yet their precise role remains unclear. Testicular MC express histidine decarboxylase (HDC), the unique enzyme responsible for histamine (HA) generation. Evidence to date supports a role for HA as a local regulator of steroidogenesis via functional H₁ and H₂ receptor subtypes (HRH1 and HRH2, respectively) present in Leydig cells.

A racemic approach to left-handed media.

It is established theoretically that racemic substances characterized by isomers with strong optical rotatory power are capable of producing negative refraction and focusing of arbitrarily polarized waves. A slab of such racemic material, while not chiral, acts just like Veselago's hypothetical left-handed media slab, providing subwavelength resolution as Pendry's ideal lens does and opening an avenue for optical and IR realizations. RF-microwave implementations in random and lattice fashion are also proposed.

Effects of heme oxygenase isozymes on Leydig cells steroidogenesis.

In the present study, we demonstrate the expression of heme oxygenase (HO) isozymes, HO-1 and HO-2 (listed as HMOX1 and HMOX2 in the MGI Database), in MA-10 Leydig tumor cells and its effect on steroidogenesis. The well-known HO inducer, hemin, increased both HO-1 and HO-2 protein levels and HO-specific activity. Induction of HO by hemin inhibited basal, hCG-, and dibutyryl cAMP (db-cAMP)-induced steroidogenesis in a reversible way.

Subwavelength imaging using conducting sheets.

Here we demonstrate that, paradoxically, subwavelength imaging can be produced by purely resistive means. Space acts like a low pass filter for highly evanescent field components, and if a sheet or thin layer of imperfectly conducting material is placed adjacent to a source, such that the layer overcomes the larger impedance of the spatial low pass filter, no relative attenuation of evanescent components is experienced at the location of the sheet. This results in near-field subwavelength imaging, which also holds for reactive sheets.

Anomalous power flow and "ghost" sources.

It is demonstrated that EM radiation from complex sources can result in real power in restricted regions of space flowing back towards the sources, thereby mimicking "ghost" sources. This counterintuitive mechanism of radiation does not rely on backward waves, as ordinary waves carry the power. Ways to harness the effect by making it directional are presented, together with selected applications, of which deception is a prime example due to the nature of the phenomenon.

Characterisation of a monoclonal antibody against Trypanosoma evansi and its application for detecting circulating antibodies.

Monoclonal antibodies were obtained against Trypanosoma evansi. The 2-4F6 IgM monoclonal antibody (Mab) was chosen for the study because of its ability to detect antigens and its specificity (as it did not recognise T. cruzi, T.

Rough ocean surface and sunglint region characteristics.

The sunglint geometrical optics equations of a statistically faceted sea, supported by the so-called interaction probability density and employing an averaging hybrid of the Cox-Munk and Mermelstein slope statistics, was successful in simulating 0.5 microm sunglint region characteristics. The results match independent experimental data, and good agreement is reported for various sea conditions and Sun locations, on sunglint amplitude, sunglint location, and azimuth range.

Three-dimensional focusing of broadband microwave beams by a layered photonic structure.

Experiment and analysis confirm that a finite layered structure involving glass layers with broad strip metallizations can be characterized by broadband negative refractionlike behavior in three dimensions. The structure is further characterized as capable of 2:1 broadband focusing of near field radiation, with verified experimental focusing bandwidth of 1:5:1. This represents a significant improvement over the two-dimensional and narrow band focusing operation of current implementations of left-handed media.

Retroreflective effect on a right angle left-handed media prism.

A different retroreflective effect which parallels that encountered with dihedral corner reflectors is found in the scattering response of a penetrable left-handed media epsilon= mu= -1 right angle prism. More significantly, no diffraction from the vertex is found to exist and hence no field singularity for the right angle wedge. Although the results are illustrated with microwaves, the concept finds applications in optics, acoustics, elasticity, and other media characterized by negative index wave propagation.

Complex source description of focal regions.

Closed-form solutions of the two-dimensional homogeneous wave equation are presented that provide focal-region descriptions corresponding to a converging bundle of rays. The solutions do have evanescent wave content and can be described as a source-sink pair or particle-antiparticle pair, collocated in complex space, with the complex location being critical in the determination of beam shape and focal region size. The wave solutions are not plagued by singularities, have a finite energy, and have a limitation on how small the focal size can get, with a penalty for limiting small spot sizes in the form of impractically high associated reactive energy.