Publications by authors named "Montanari M"

Background: Mitochondrial dysfunction and oxidative stress are central mechanisms in the progression of neurodegenerative diseases. This study first evaluated the toxicity of Q-Der (Q10-diacetate), a derivative of Coenzyme Q10, in HT22 hippocampal neurons under normal and oxidative stress conditions.

Methods: HT22 cells were treated with Q-Der at 2.

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Background: Platelet (PLT) transfusion is an essential strategy to prevent bleeding in children with thrombocytopenia associated to cancer treatment. However, data on optimal pediatric dosing and transfusion thresholds are limited.

Methods: This retrospective study analyzed data from 607 pediatric patients with hematologic malignancies, nonmalignant disorders, and solid tumors who developed hypoproliferative thrombocytopenia during therapy.

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One of the most pressing challenges facing society today is the rising prevalence of physical and cognitive frailty. This geriatric condition makes older adults more vulnerable to disability, illness, and a heightened risk of mortality. In this scenario, Parkinson's disease (PD) and geriatric frailty, which share several common characteristics, are becoming increasingly prevalent worldwide, underscoring the urgent need for innovative strategies.

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Bioprinting of nanohydroxyapatite (nHA)-based bioinks has attracted considerable interest in bone tissue engineering. However, the role and relevance of the physicochemical properties of nHA incorporated in a bioink, particularly in terms of its printability and the biological behavior of bioprinted cells, remain largely unexplored. In this study, two bioinspired nHAs with different chemical compositions, crystallinity, and morphologies were synthesized and characterized: a more crystalline, needle-like Mg-doped nHA (N-HA) and a more amorphous, rounded Mg- and CO-doped nHA (R-HA).

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Full-arch zirconia restorations on implants have gained popularity due to zirconia's strength and aesthetics, yet they are still associated with challenges like structural fractures, peri-implant complications, and design misfits. Advances in CAD/CAM and digital workflows offer potential improvements, but a technique that consistently addresses these issues in fixed, full-arch, implant-supported prostheses is needed. This novel technique integrates a facially and prosthetically driven treatment approach, which is divided into three phases: data acquisition, restoration design, and manufacturing/delivery.

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Background: Adherence to optimal practices in the preparation and issuance of pediatric blood components can significantly influence patient care outcomes. This study aims to examine the blood banking procedures across prominent Italian children's hospitals, with the goal of identifying both consistent and potentially divergent standards within this field.

Materials And Methods: A survey was conducted among the blood banks affiliated with the Italian Association of Pediatric Hospitals.

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Multiple gut antimicrobial mechanisms are coordinated in space and time to efficiently fight foodborne pathogens. In , production of reactive oxygen species (ROS) and antimicrobial peptides (AMPs) together with intestinal cell renewal play a key role in eliminating gut microbes. A complementary mechanism would be to isolate and treat pathogenic bacteria while allowing colonization by commensals.

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In an era when ecological and environmental needs and responsibilities apply pressure on the world's countries and sustainability takes centre stage, ecologic/environmental (E/E) laboratories stand as beacons of scientific inquiry, innovating, optimising, and applying various tests for a better knowledge of our natural resources and the quality status of ecosystems. The purpose of this review is to provide an overview of the use of flow cytometry (FC) as a tool for assessing environmental quality, mainly using living organisms and their biological changes as bioindicators. Cytometric approaches applied to both marine and terrestrial ecosystems ensure the detection of biochemical and functional status of the cells composing either an organ thereof or the organism itself.

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Article Synopsis
  • * This modified technique involves specific skin incisions and a unique order for removing carpal bones, aiming to improve visibility and reduce complications related to tethered tendons.
  • * Results from a study of 38 patients showed significant improvement in wrist function after using this new approach, particularly beneficial for cases with carpal tunnel syndrome or complicated wrist injuries.
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L. essential oil has attracted the interest of the scientific community thanks to its numerous biological activities. Several studies have evaluated EOs as alternative therapeutic approaches to limit the use of antibiotics; the present study aimed to evaluate the inhibitory and bactericidal activity of the essential oils obtained from the leaves and inflorescences of two hemp genotypes against twenty-one multidrug-resistant, methicillin-resistant strains isolated from canine clinical samples.

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  • * The update reflects new studies that reinforce the effectiveness of ECP in treating GVHD, with the goal of establishing consistent criteria for its use in both adults and children across Italy while ensuring patient safety and high-quality care.
  • * The guideline development involved a diverse group of specialists, including transfusionists and hematologists, with oversight by a hematologist skilled in systematic reviews, and also included input from a patient representative
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, identified in 2006, represents a challenging case of bacterial taxonomic identification because of its high similarity to . In this context, neither mass spectrometry (MS) nor 16S gene analysis cannot precisely reveal the difference between the two species. In our study, the sensitivity to antibiotics of isolated from blood culture was tested, and the investigation of the bacterial genome was performed by Multi-Locus Sequence Typing (MLST) and Whole-Genome Next-Generation Sequencing (WG-NGS).

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  • The study investigates the microbial contamination of industrial hemp, highlighting risks of pathogens like E. coli and Salmonella, particularly in plants grown outdoors.
  • Seven Italian hemp genotypes were analyzed over three years in different locations, revealing that most samples failed to meet European microbiological safety standards.
  • Findings suggest a negative correlation between CBD levels and microbial contamination, indicating that higher CBD concentrations may offer some protection against harmful microbes.
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The valorization of discarded wool from dairy sheep breeding is a challenging issue. The most proposed strategies lie in the processing of keratin extracted from wool without reducing the molecular weight of the protein chains (the high molecular weight-HMW keratin). Here, the HMW keratin has been spun for the first time by solution blow spinning.

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Extracellular vesicles (EVs) are promising natural nanocarriers for the delivery of therapeutic agents. As with any other kind of cell, red blood cells (RBCs) produce a limited number of EVs under physiological and pathological conditions. Thus, RBC-derived extracellular vesicles (RBCEVs) have been recently suggested as next-generation delivery systems for therapeutic purposes.

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The dynamics of microtubule-mediated protrusions, termed Interplanar Amida Network (IPAN) in pupal wing, involve cell shape changes. The molecular mechanisms underlying these processes are yet to be fully understood. This study delineates the stages of cell shape alterations during the disassembly of microtubule protrusions and underscores the pivotal role of α-Spectrin in driving these changes by regulating both the microtubule and actomyosin networks.

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Sexually transmitted infections (STIs) have seen a considerable increase in the last years and given the health burden they may represent from both a personal and community perspective, they require surveillance and prevention programmes based on a timely and decentralized diagnosis. In this context, user-friendly rapid molecular tests may represent a good trade-off between diagnostic accuracy, accessibility and affordability. In this study we evaluated the diagnostic performance of a new real-time LAMP (Loop Mediated Isothermal Amplification) method for the rapid detection and differentiation of 7 major sexually transmissible pathogens by analysing real clinical samples (genital and extra-genital matrices) from individuals with suspected STIs.

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  • Hemp cultivation in Europe has grown significantly from 20,450 hectares in 2015 to 33,020 hectares in 2022, driven by diverse market opportunities in food, cosmetics, and energy.
  • Mycotoxin contamination in hemp seeds presents a concern, particularly with the presence of fungal pathogens that can affect quality and safety.
  • A survey of hemp seed samples in Italy from 2018 to 2022 revealed low levels of regulated mycotoxins, but concerningly high levels of alternariols and their associated toxins, influenced by weather and plant variety.
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Promoting neural cell proliferation may represent an important strategy for enhancing brain repair after developmental brain injury. The present study aimed to assess the effects of melatonin on cell proliferation after an ischemic injury in the developing hippocampus, focusing on cell cycle dynamics. After in vivo neonatal hypoxia-ischemia (HI), hippocampal cell cycle dynamics were assessed by flow cytometry, together with histological evaluation of dentate gyrus cellularity and proliferation.

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The survival of animals depends, among other things, on their ability to identify threats in their surrounding environment. Senses such as olfaction, vision and taste play an essential role in sampling their living environment, including microorganisms, some of which are potentially pathogenic. This study focuses on the mechanisms of detection of bacteria by the Drosophila gustatory system.

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Comprehensive analysis of cellular dynamics during the process of morphogenesis is fundamental to understanding the principles of animal development. Despite recent advancements in light microscopy, how successive cell shape changes lead to complex three-dimensional tissue morphogenesis is still largely unresolved. Using in vivo live imaging of Drosophila wing development, we have studied unique cellular structures comprising a microtubule-based membrane protrusion network.

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spp. are an important opportunistic pathogen that can represent a possible cause of severe infections, especially in immunocompromised individuals. The clinical impact of spp.

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Several studies have reported the high bioindication capacity of Isopoda (Crustacea, Oniscidea), which is related to their important ability to accumulate contaminants, usefulness in soil ecotoxicology and bioindication activities. Any change in the isopod population, diversity and life cycle can indicate relevant pollution levels. The analysis of target tissues, such as the hepatopancreas, is another emerging approach (from a cytologic/histological level) to detect contaminant accumulation from different sources.

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Loss-of-function mutations in the GNAL gene are responsible for DYT-GNAL dystonia. However, how GNAL mutations contribute to synaptic dysfunction is still unclear. The GNAL gene encodes the Gα protein, an isoform of stimulatory Gα enriched in the striatum, with a key role in the regulation of cAMP signaling.

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Purpose: Aims of our study were to define whether the great tuberosity (GT) positioning fixation in reverse shoulder arthroplasty (RSA) after proximal humeral fracture (PHF) could predict tuberosity healing and its impact on clinical outcomes.

Methods: We enrolled 59 patients treated with cementless reverse shoulder arthroplasty after PHF in our institute between 2012 and 2018. The mean follow-up was 57 months (± 23.

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