Publications by authors named "Monisha Thiyagarajan"
Article Synopsis
- The film rehydration method is widely used for incorporating membrane proteins (MPs) into block copolymer membranes, but inconsistencies during rehydration lead to mixed populations of proteopolymersomes, complicating device performance.
- This study focused on optimizing the rehydration process for improved MP reconstitution, specifically using AquaporinZ (AqpZ), which is known for efficiently transporting water molecules.
- Findings revealed that prehydrating the membrane with buffer before adding AqpZ resulted in the best reconstitution outcomes, enhancing vesicle formation and maintaining AqpZ functionality, while emphasizing the significance of membrane fluidity during integration.
