Poly(vinyl alcohol)/hydroxyapatite Monolithic In-Needle Extraction (MINE) device: Preparation and examination of drug affinity.

Polymer-ceramic materials based on poly(vinyl alcohol) (PVA) and hydroxyapatite were applied as sorption material in Monolithic In-Needle Extraction (MINE) device. The presented device provides new possibilities for the examination of bisphosphonates affinity for bone and will be a helpful tool in evaluation of potential antiresorptive drugs suitability. A ceramic part of monoliths was prepared by incorporation of hydroxyapatite (HA) into the reaction mixture or by using a soaking method (mineralization of HA on the PVA).

Experimental and in silico investigations of organic phosphates and phosphonates sorption on polymer-ceramic monolithic materials and hydroxyapatite.

A method based on experimental and in silico evaluations for investigating interactions of organic phosphates and phosphonates with hydroxyapatite was developed. This quick and easy method is used for determination of differences among organophosphorus compounds of various structures in their mineral binding affinities. Empirical sorption evaluation was carried out using liquid chromatography with tandem mass spectrometry or UV-VIS spectroscopy.

Polymer-ceramic Monolithic In-Needle Extraction (MINE) device: Preparation and examination of drug affinity.

Polymer-ceramic materials were placed in the in-needle device. Polymer-ceramic Monolithic In-Needle Extraction (MINE) device is an extraction device used in sample preparation step but, on the other hand, it can be a tool for examination of interactions between potential antiresorptive drugs and bones. MINE device was used as tool for determination of bisphosphonate affinity to hydroxyapatite.

Optimization of the in-needle extraction device for the direct flow of the liquid sample through the sorbent layer.

In-needle extraction was applied for preparation of aqueous samples. This technique was used for direct isolation of analytes from liquid samples which was achieved by forcing the flow of the sample through the sorbent layer: silica or polymer (styrene/divinylbenzene). Specially designed needle was packed with three different sorbents on which the analytes (phenol, p-benzoquinone, 4-chlorophenol, thymol and caffeine) were retained.

An in-needle extraction technique in determination of organic compounds released from dental tissue conditioners incubated in artificial saliva.

The use of an in-needle technique for direct isolation of analytes from real liquid samples is a new proposal. The in-needle technique has been relatively seldom used for direct sampling of liquid matrix through the needle. In this work the in-needle technique has been applied for the determination of compounds evolved to artificial saliva from dental prosthetic materials.

Solubility parameter used to predict the effectiveness of monolithic in-needle extraction (MINE) device for the direct analysis of liquid samples.

The sorbent/eluent systems combined from three macroporous poly(styrene-divinylbenzene) (PS-DVB) monoliths and four solvents as eluents were used for the extraction of phenol, 4-chlorophenol and p-benzochinon from water samples. Monolithic in-needle extraction (MINE) devices were used in the preparation of a series of test water samples for chromatographic analysis. The extraction of phenolic compounds from water samples was carried out by pumping liquid samples through the MINE device.

Preparation and examination of monolithic in-needle extraction (MINE) device for the direct analysis of liquid samples.

Combination of extraction and chromatographic techniques opens NEW possibilities in sample preparation area. Macroporous poly(styrene-divinylbenzene) (PS-DVB) monoliths were prepared by in situ polymerization in stainless steel needles. The surface of stainless steel needle was modified earlier by the silane coupling agent.

Effectiveness of in-needle extraction device for liquid samples.

In this work in-needle technique as the method for liquid samples preparation which faces with the problem of high flow resistance produced by a sorbent layer is considered. The influence of parameters (needle size, sorbent particle size and length of sorbent layer) of in-needle device on the flow rate was examined. Acceptable sampling conditions were selected on the basis of experiments and their chemometric evaluation.