Screening Clinical, Laboratory and Host Markers for Diagnosis of Disease Severity in Clinical Samples.

Unlabelled: Malaria is one of the most infectious disease that affects lives of million people throughout the world. Recently, there are several reports which indicate causing severe disease in infected patients from different parts of the world. For disease severity, the data related to immunological and inflammatory status in human host is very limited.

Seroprevalence and characterization of Epstein-Barr virus exposure among paediatric population.

The study explored Epstein Barr Virus (EBV) exposure in 244 children using EBV-specific serology. Seroprevalence of EBV was 75-80%. Past infection and primary infection were observed in 52.

Trends in clinical features and severity of Plasmodium vivax malaria among children at tertiary care center in North India.

Background: Malaria is a significant cause of morbidity and mortality in adults and children. Plasmodium falciparum is the primary cause of severe malaria, but recently Plasmodium vivax is also recognized to cause severe malaria-associated morbidity and mortality. The study focuses on determining the mortality related to severity parameters in individuals under 12 years and their critical presentation in P.

A Case-Control Study of Serum and Tissue Catalase among Morphological Variants of Vitiligo.

Background: Role of oxidative stress in the pathogenesis of vitiligo.

Aim: Estimation of serum and tissue catalase levels in morphological variants of vitiligo.

Settings And Design: A prospective case-control study was conducted in the outpatient department of Dermatology in Safdarjung hospital.

Molecular characterization of the severe malaria with typhoid co-infection: A case report.

Malaria and typhoid co-infections can be a serious public health issue in tropical countries leading to incorrect diagnosis due to overlapping clinical presentations of malaria and typhoid and hence, causing a delay in implementing the appropriate treatment regimen for these concurrent infections. This study reports a case of six-year-old female child co-infected with severe malaria (Plasmodium falciparum) and typhoid (Salmonella typhi) diagnosed by rapid malaria antigen test (RMAT) and blood culture respectively. Further, analysis of the chloroquine resistance gene Pfcrt for the falciparum demonstrated the presence of K76T mutant allele in pfcrt gene with high IC50 (150nM) for chloroquine (CQ) drug.

Scrub Typhus Co-Infections in a Young Boy With Varicella and Malaria: A Rare Case Report.

Scrub typhus is one of the leading causes of acute febrile illness of unknown origin in India. Though several co-infections of other vector-borne diseases have been described in the literature, few such cases have been described in children. As such, it is challenging to diagnose scrub typhus alone and becomes that more complicated when a varicella infection precedes it.

Studying the disease severity in clinical isolates of Plasmodiumvivax.

Among the human malaria Plasmodium species, Plasmodium vivax is the most widespread species globally. In recent times, this historically benign species is now being recognized as also responsible for severe malaria infections in humans. Hence, a deeper insight of P.

Development of Two-Tube Loop-Mediated Isothermal Amplification Assay for Differential Diagnosis of and and Its Comparison with Loopamp™ Malaria.

To strengthen malaria surveillance, field-appropriate diagnostics requiring limited technical resources are of critical significance. Loop-mediated isothermal amplification (LAMP) based malaria diagnostic assays are potential point-of-care tests with high sensitivity and specificity and have been used in low-resource settings. -specific consensus repeat sequence (CRS)-based and -specific 18S rRNA primers were designed, and a two-tube LAMP assay was developed.

Pancytopenia with hemophagocytic lymphohistiocytosis in : A unusual presentation.

Hematological manifestations such as anemia and thrombocytopenia are known complications in malaria. Here, we report two cases presented as pancytopenia with hepatosplenomegaly and initial diagnosis kept as hematological malignancy like leukemia but later on its diagnosed as malaria-associated hemophagocytic lymphohistiocytosis which is a rare entity. The aim of this report is to draw the attention of physicians, especially in tropical countries such as India and Sub-Saharan nations to keep in mind this uncommon presentation of malaria, though the exact pathophysiological mechanism still remains obscure.

Neonatal Chikungunya: A case series.

Chikungunya virus is an RNA virus that belongs to the family Togaviridae, genus , transmitted by Aedes mosquitoes. The disease usually manifests as fever, arthralgia and petechial or maculopapular rash. The illness is usually self-limiting.

Assessing the in vitro sensitivity with associated drug resistance polymorphisms in Plasmodium vivax clinical isolates from Delhi, India.

The drug resistance of Plasmodium vivax in clinical cases remains largely unknown till date because of the difficulty in diagnosing the resistant P. vivax strains. The present study was undertaken to determine the prevalence of mutant alleles in drug resistance genes viz P.

Severe vivax malaria trends in the last two years: a study from a tertiary care centre, Delhi, India.

Background: Plasmodium vivax, once considered benign species, is recently being recognised to be causing severe malaria like Plasmodium falciparum. In the present study, the authors report the trends in malaria severity in P. vivax among patients from a Delhi government hospital.

Allelic variation of msp-3α gene in Plasmodium vivax isolates and its correlation with the severity of disease in vivax malaria.

Malaria is a global socio-economic burden of which Plasmodium vivax contributes for about 70-80 million cases on an annual basis worldwide and 60-65% cases in India. Diversity observed in highly polymorphic Merozoite Surface Protein-3α (msp-3α) encoded by MSP-3 gene family, has been used efficiently for genotyping of P. vivax infection.

Early treatment failure in concurrent dengue and mixed malaria species infection with suspected resistance to artemisinin combination therapy from a tertiary care center in Delhi: a case report.

Background: Concurrent dengue and mixed malaria infections in a single patient present with overlapping clinical manifestations which pose a diagnostic challenge and management dilemma in areas of common endemicities.

Methods: We report a case of a young male who tested positive for both and along with dengue infection. He showed signs of early treatment failure to artemisinin combination therapy (artesunate with sulfadoxine+pyrimethamine).

HIV-TB Cross-referral and Collaborative Strategy: 8 Years of Our Experience from An Urban Health Centre in North India.

Background: Human immunodeficiency virus (HIV) and tuberculosis (TB) epidemics continue to fuel each other and with dual infections with these two deadly diseases on the rise, it becomes imperative to devise effective HIV-TB collaborative strategies. The present study was designed to evaluate the existing HIV-TB cross-referral mechanism at an urban health centre; to determine HIV sero-prevalence among pulmonary TB patients referred from chest clinic to the integrated counselling and testing centre (ICTC); and to evaluate the TB suspects referred from ICTC to the chest clinic for a possible TB aetiology.

Methods: The present study was a retrospective analysis of HIV-TB cross-referrals whereby a line list of all the patients referred under this strategy from January 2006 to December 2013 was retrieved and analysed.

Routine Screening for Rubella and CMV Antibodies During Pregnancy: Is it Justifiable?

Background: Rubella and cytomegalovirus (CMV) screening during pregnancy is routinely carried out in India. However, its value has been questioned due to the absence of clearly effective intervention.

Objectives: This retrospective study evaluates the usefulness of rubella and CMV antibody screening during pregnancy.

Onychomycosis due to rhizomucor in psoriatic patient with HIV infection.

Onychomycosis is frequently seen in dermatological clinical practice worldwide. The causative agents are usually two pathogenic groups of fungi namely, dermatophytes and yeasts of the genus Candida. In some cases, non-dermatophytic molds belonging to different genera and species may be the etiological agents.

Clinico-laboratory findings of patients during dengue outbreak from a tertiary care hospital in Delhi.

There are outbreaks of dengue every year in India. They vary in the predominant serotype involved, clinical features and predominant laboratory findings. This study of the 2006 outbreak in Delhi highlights clinical features and laboratory parameters of dengue cases and compares the clinical features among the adult and paediatric age groups.

Immunodiagnosis of dengue virus infection using saliva.

Keeping in view the complications and the case fatality associated with dengue virus, several serologic tests have been developed. However, the major drawback of these serologic tests is the need for a venous blood sample obtained by invasive venipuncture. As a noninvasive alternative, saliva provides a body fluid that contains antibodies of diagnostic importance.