Publications by authors named "Monier Alhadi Abdelrahman Mohammed"

Article Synopsis
  • * The specific enzyme (PGA) was successfully immobilized through a chemical reaction, and optimal conditions for this process were identified with a 93.71% enzyme activity recovery and significant loading capacity.
  • * The immobilized PGA demonstrated better stability and efficiency, retaining 58% of its initial activity after 11 uses, suggesting its potential for practical applications in various fields.
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Preparation of biocatalyst dependent on immobilized penicillin G acylase (PGA) was of substantial importance for proteomic research, organic synthesis, and industrial applications. Herein, we developed an easy method for nano-magnetic composite to immobilize PGA. FeO nano-magnetic particles were co-precipitated with Fe and Fe in an ammonia solution (NH) and treated with silicon dioxide (SiO), which was developed using the sol-gel process.

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In this work, Fe O nanoparticles (NPs) were coated with polydopamine (PDA) to structure Fe O @PDA NPs by the spontaneous oxygen-mediated self-polymerization of dopamine (DA) in an aqueous solution of pH = 8.5. The fabricated Fe O @PDA NPs were grafted by glutaraldehyde to realize the immobilization of penicillin G acylase (PGA) under mild conditions.

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