Household Food Metabolism: Losses, Waste and Environmental Pressures of Food Consumption at the Regional Level in Spain.

Dealing with an increasing population is challenging the global food system not only in productive terms, but also through the associated environmental pressures. A growing diagnostic effort is being made by global and national agencies. Innovative approaches are needed to support effective policy efforts.

Functional expression of a single-chain antibody to ErbB-2 in plants and cell-free systems.

Background: Aberrant signaling by ErbB-2 (HER 2, Neu), a member of the human Epidermal Growth Factor (EGF) receptor family, is associated with an aggressive clinical behaviour of carcinomas, particularly breast tumors. Antibodies targeting the ErbB-2 pathway are a preferred therapeutic option for patients with advanced breast cancer, but a worldwide deficit in the manufacturing capacities of mammalian cell bioreactors is foreseen.

Methods: Herein, we describe a multi-platform approach for the production of recombinant Single chain Fragments of antibody variable regions (ScFvs) to ErbB-2 that involves their functional expression in (a) bacteria, (b) transient as well as stable transgenic tobacco plants, and (c) a newly developed cell-free transcription-translation system.