Exploring positive welfare measures: preliminary findings from a prototype protocol in loose housing dairy cattle farms.

Introduction: Following the increasing interest about the development of indicators of positive welfare and affective state in farm animals, the aim of this research is to present some preliminary results on the application of a prototype protocol based exclusively on positive welfare measures and to suggest potential benefits that can promote positive welfare.

Methods: The protocol was applied in 20 loose housing dairy cattle farms (6 on deep litter with straw, 14 in cubicles) and included only indicators of positive welfare and emotional states: feeding and resting synchronization, rumination during resting, comfortable lying postures, no visible eye white, relaxed ear postures, percentage of cow contacts with humans in the Avoidance Distance test. Potential benefits in terms of housing, feeding and management were then related to these variables (Mann-Whitney U test).

A methodological approach to compare continuous and instantaneous sampling and two methods to deal with animals out of sight on dairy cattle behavior and interaction with their calf in the first hours post-partum.

Animal behavior can provide useful information about animal welfare, but methods and tools used to gather behavioral data and data treatment can influence the results. Therefore, this study was carried out on dairy cow () behavior and interaction with calves early post-partum aiming at comparing two sampling rules, namely continuous and instantaneous sampling at scan intervals of 30 s, 1, 2, 3, 4, 5, and 10 min, and of two methods to deal with out of sight animals. The study was based on three assumptions: (1) continuous sampling provides the most complete and accurate data, allowing the observation of seldom behaviors and short events; (2) instantaneous sampling can provide accurate measurements of frequency and duration, especially at short scan intervals; (3) differences in behavioral results may occur depending on whether a correction for time out of sight is applied or not.

A systematic approach to defining and verifying descriptors used in the Qualitative Behavioural Assessment of sows.

Qualitative Behaviour Assessment (QBA) is a welfare evaluation tool that uses a holistic approach to capturing an animal's emotional state. Lists of QBA descriptors validated to assess pig welfare exist, but their definitions are often not described in peer-reviewed literature and the processes used to develop definitions are lacking. The objective of this study is to detail a systematic approach to creating clear definitions for a pre-existing fixed list of QBA descriptors and test their application.

Humans and Goats: Improving Knowledge for a Better Relationship.

There is consensus that the quality of the human-animal relationship (HAR) is relevant to guarantee appropriate levels of animal welfare. Given the impact that HAR may have on both goats and human beings, the aim of the present review is to elucidate: (1) how humans and goats communicate; (2) which are the factors affecting human-goat interactions; (3) how we can measure the quality of this relationship. The systematic review led to the selection of 58 relevant articles.

Feasibility and Reliability of the AWIN Welfare Assessment Protocol for Dairy Goats in Semi-extensive Farming Conditions.

The aim of this study was to test the feasibility and reliability of the Animal Welfare Indicators (AWIN) protocol for welfare assessment of dairy goats when applied to semi-extensive farming conditions. We recruited 13 farms located in the NW Italian Alps where three assessors individually and independently applied a modified version of the AWIN welfare assessment protocol for goats integrated with some indicators derived from the AWIN welfare assessment protocol for sheep. The applied protocol consisted of nine individual-level (body condition score, hair coat condition, abscesses, overgrown claws, udder asymmetry, fecal soiling, nasal discharge, ocular discharge, and improper disbudding) and seven group-level (severe lameness, Qualitative Behavior Assessment-QBA, thermal stress, oblivion, Familiar Human Approach Test-FHAT, synchrony at grazing, synchrony at resting) animal-based indicators.

Evaluation of Inter-Observer Reliability of Animal Welfare Indicators: Which Is the Best Index to Use?

This study focuses on the problem of assessing inter-observer reliability (IOR) in the case of dichotomous categorical animal-based welfare indicators and the presence of two observers. Based on observations obtained from Animal Welfare Indicators (AWIN) project surveys conducted on nine dairy goat farms, and using udder asymmetry as an indicator, we compared the performance of the most popular agreement indexes available in the literature: Scott's π, Cohen's k, kPABAK, Holsti's H, Krippendorff's α, Hubert's Γ, Janson and Vegelius' J, Bangdiwala's B, Andrés and Marzo's ∆, and Gwet's γ(AC1). Confidence intervals were calculated using closed formulas of variance estimates for π, k, kPABAK, H, α, Γ, J, ∆, and γ(AC1), while the bootstrap and exact bootstrap methods were used for all the indexes.

What's in a Meow? A Study on Human Classification and Interpretation of Domestic Cat Vocalizations.

Although the domestic cat () is probably the most widespread companion animal in the world and interacts in a complex and multifaceted way with humans, the human-cat relationship and reciprocal communication have received far less attention compared, for example, to the human-dog relationship. Only a limited number of studies have considered what people understand of cats' human-directed vocal signals during daily cat-owner interactions. The aim of the current study was to investigate to what extent adult humans recognize cat vocalizations, namely meows, emitted in three different contexts: waiting for food, isolation, and brushing.

Intrinsic and Extrinsic Quality Attributes of Fresh and Semi-Hard Goat Cheese from Low- and High-Input Farming Systems.

In this study, we investigated the lipid composition of fresh and semi-hard goat cheese produced in three Italian farms as well as the welfare assessment of goats reared in these farms. The fatty acid (FA) profile of cheese samples were found to be strictly related to the livestock system. Cheese collected from farms in which goats were allowed to graze and were fed diets with a higher forage/concentrate (F/C) ratio showed a FA profile represented by higher contents of health-promoting fatty acids.

The role of claw deformation and claw size on goat lameness.

Lameness due to claw overgrowth remains one of the main welfare challenges in dairy goat farms. Although claw trimming is a crucial part of the solution, most times there is a delay in its implementation, with no perceived consequences. The goal of this cross-sectional study was to assess the correlation between the size and deformation of dairy goats claws with lameness score.

Welfare Assessment on Pasture: A Review on Animal-Based Measures for Ruminants.

Outdoor and extensive farming systems allow animals to behave in a natural way and are often perceived as welfare friendly. Nonetheless, the natural environment poses multiple challenges to the welfare of animals, sometimes hampering their capacity to cope. Welfare assessment in outdoor and extensive systems has been rarely investigated, and little is known about the most appropriate indicators.

Evaluation of Pain Mitigation Strategies in Goat Kids after Cautery Disbudding.

Nowadays, most of the goat milk production in developed countries is done in intensive indoors production systems. In these systems, procedures such as disbudding are performed routinely. Disbudding is done in young goat kids and is a recognised as a painful procedure.

How Can We Assess Positive Welfare in Ruminants?

Until now, most research has focused on the development of indicators of negative welfare, and relatively few studies provide information on valid, reliable, and feasible indicators addressing positive aspects of animal welfare. However, a lack of suffering does not guarantee that animals are experiencing a positive welfare state. The aim of the present review is to identify promising valid and reliable animal-based indicators for the assessment of positive welfare that might be included in welfare assessment protocols for ruminants, and to discuss them in the light of the five domains model, highlighting possible gaps to be filled by future research.

Automatic Classification of Cat VocalizationsEmitted in Different Contexts.

Cats employ vocalizations for communicating information, thus their sounds can carry a widerange of meanings. Concerning vocalization, an aspect of increasing relevance directly connected withthe welfare of such animals is its emotional interpretation and the recognition of the production context.To this end, this work presents a proof of concept facilitating the automatic analysis of cat vocalizationsbased on signal processing and pattern recognition techniques, aimed at demonstrating if the emissioncontext can be identified by meowing vocalizations, even if recorded in sub-optimal conditions.

Understanding Cows' Emotions on Farm: Are Eye White and Ear Posture Reliable Indicators?

Understanding the emotions of dairy cows is primarily important in enhancing the level of welfare and provide a better life on farm. This study explored whether eye white and ear posture can reliably contribute to interpret valence and arousal of emotions in dairy cows. The research was conducted in five Italian dairy farms.

Text Mining Analysis to Evaluate Stakeholders' Perception Regarding Welfare of Equines, Small Ruminants, and Turkeys.

Welfare of animals significantly depends on how stakeholders perceive their needs and behave in a way to favor production systems that promote better welfare outcomes. This study aimed at investigating stakeholders' perception of the welfare of equines, small ruminants, and turkeys using text mining analysis. A survey composed by open-ended questions referring to different aspects of animal welfare was carried out.

The Use of Qualitative Behaviour Assessment for the On-Farm Welfare Assessment of Dairy Goats.

This research investigated whether using qualitative behaviour assessment (QBA) with a fixed list of descriptors may be related to quantitative animal- (ABM) and resource-based (RBM) measures included in the AWIN (Animal Welfare Indicators) welfare assessment prototype protocol for goats, tested in 60 farms. A principal component analysis (PCA) was conducted on QBA descriptors; then PCs were correlated to some ABMs and RBMs. Subsequently, a combined PCA merged QBA scores, ABMs and RBMs.

On-FarmWelfare Assessment Protocol for Adult Dairy Goats in Intensive Production Systems.

Within the European AWIN project, a protocol for assessing dairy goats' welfareon the farm was developed. Starting from a literature review, a prototype includinganimal-based indicators covering four welfare principles and 12 welfare criteria was set up.The prototype was tested in 60 farms for validity, reliability, and feasibility.

Feasibility and validity of animal-based indicators for on-farm welfare assessment of thermal stress in dairy goats.

This investigation tested the feasibility and validity of indicators of cold and heat stress in dairy goats for on-farm welfare assessment protocols. The study was performed on two intensive dairy farms in Italy. Two different 3-point scale (0-2) scoring systems were applied to assess cold and heat stress.