Task and Timing Effects in Argument Role Sensitivity: Evidence From Production, EEG, and Computational Modeling.

Comprehenders generate expectations about upcoming lexical items in language processing using various types of contextual information. However, a number of studies have shown that argument roles do not impact neural and behavioral prediction measures. Despite these robust findings, some prior studies have suggested that lexical prediction might be sensitive to argument roles in production tasks such as the cloze task or in comprehension tasks when additional time is available for prediction.

Fatty acid-binding protein 5 is a functional biomarker and indicator of ferroptosis in cerebral hypoxia.

The progression of human degenerative and hypoxic/ischemic diseases is accompanied by widespread cell death. One death process linking iron-catalyzed reactive species with lipid peroxidation is ferroptosis, which shows hallmarks of both programmed and necrotic death in vitro. While evidence of ferroptosis in neurodegenerative disease is indicated by iron accumulation and involvement of lipids, a stable marker for ferroptosis has not been identified.

Molecular Determinants of Calcitriol Signaling and Sensitivity in Glioma Stem-like Cells.

Glioblastoma is the most common primary brain cancer in adults and represents one of the worst cancer diagnoses for patients. Suffering from a poor prognosis and limited treatment options, tumor recurrences are virtually inevitable. Additionally, treatment resistance is very common for this disease and worsens the prognosis.

Grammatical dependencies shape compositional units in sentence production.

Five recall-based structural priming experiments tested the predictions about dative structural priming derived from a new theory of structure building in sentence production. When both prime and target sentences contained direct object filler-gap dependencies, repeating a dative verb enhanced dative priming (the lexical boost). In contrast, the lexical boost was not observed when only target sentences contained object filler-gap dependencies.

Current challenges and future directions for engineering extracellular vesicles for heart, lung, blood and sleep diseases.

Extracellular vesicles (EVs) carry diverse bioactive components including nucleic acids, proteins, lipids and metabolites that play versatile roles in intercellular and interorgan communication. The capability to modulate their stability, tissue-specific targeting and cargo render EVs as promising nanotherapeutics for treating heart, lung, blood and sleep (HLBS) diseases. However, current limitations in large-scale manufacturing of therapeutic-grade EVs, and knowledge gaps in EV biogenesis and heterogeneity pose significant challenges in their clinical application as diagnostics or therapeutics for HLBS diseases.

Cross-clause planning in Nungon (Papua New Guinea): Eye-tracking evidence.

Hundreds of languages worldwide use a sentence structure known as the "clause chain," in which 20 or more clauses can be stacked to form a sentence. The Papuan language Nungon is among a subset of clause chaining languages that require "switch-reference" suffixes on nonfinal verbs in chains. These suffixes announce whether the subject of each upcoming clause will differ from the subject of the previous clause.

Visualizing transfer of microbial biomolecules by outer membrane vesicles in microbe-host-communication in vivo.

The intestinal microbiota influences mammalian host physiology in health and disease locally in the gut but also in organs devoid of direct contact with bacteria such as the liver and brain. Extracellular vesicles (EVs) or outer membrane vesicles (OMVs) released by microbes are increasingly recognized for their potential role as biological shuttle systems for inter-kingdom communication. However, physiologically relevant evidence for the transfer of functional biomolecules from the intestinal microbiota to individual host cells by OMVs in vivo is scarce.

SnRNA sequencing defines signaling by RBC-derived extracellular vesicles in the murine heart.

Extracellular vesicles (EVs) mediate intercellular signaling by transferring their cargo to recipient cells, but the functional consequences of signaling are not fully appreciated. RBC-derived EVs are abundant in circulation and have been implicated in regulating immune responses. Here, we use a transgenic mouse model for fluorescence-based mapping of RBC-EV recipient cells to assess the role of this intercellular signaling mechanism in heart disease.

Filling the gap in gap-filling: Long-distance dependency formation in sentence production.

In a sentence like Who does the artist think chased the chef?, the who at the beginning depends on the last bit of the sentence, chased the chef. This is an instance of a long-distance dependency. What is the nature of the cognitive process that allows speakers to produce sentences that include distant elements that form dependencies? In four experiments, speakers described drawings that elicited long-distance dependencies.

Learning structural alternations: What guides learners' generalization?

Some word-order alternations observed across the world's languages are constrained by specific verb choice, whereas one type of word-order alternation (i.e., scrambling) frequently seen in free word order languages is not lexically-dependent on the verb.

Beyond linear order: The role of argument structure in speaking.

The current study examines how speakers plan sentences in which two words that form hierarchical dependency relationships - arguments and verbs - appear far apart in linear distance, to investigate how linear and hierarchical aspects of sentences simultaneously shape sentence planning processes. The results of six extended picture-word interference experiments suggest that speakers retrieve sentence-final verbs before the articulation of their sentence-initial patient or theme arguments, but not agent arguments, and before retrieving sentence-medial nouns inside modifiers. These results suggest that the time-course of sentence planning reflects hierarchically-defined dependency relationships over and above linear structure.

Extracellular vesicles for remote brain repair.

Recovery of brain function lost to disease or in old age is a challenging task in regenerative medicine. In the last two decades, therapeutic strategies have undergone significant shifts by a succession of major discoveries from adult neural stem cells and neurogenesis to the development of induced pluripotent stem cells to technologies for reprogramming cells in vitro and in vivo. Now, extracellular vesicles, small membrane-bound vesicles released by all cells and containing lipids, proteins, and nucleic acids, emerge as the next major technological opportunity.

Neuronal activity triggers uptake of hematopoietic extracellular vesicles in vivo.

Communication with the hematopoietic system is a vital component of regulating brain function in health and disease. Traditionally, the major routes considered for this neuroimmune communication are by individual molecules such as cytokines carried by blood, by neural transmission, or, in more severe pathologies, by the entry of peripheral immune cells into the brain. In addition, functional mRNA from peripheral blood can be directly transferred to neurons via extracellular vesicles (EVs), but the parameters that determine their uptake are unknown.

Syntactic category constrains lexical competition in speaking.

We report two experiments that suggest that syntactic category plays a key role in limiting competition in lexical access in speaking. We introduce a novel sentence-picture interference (SPI) paradigm, and we show that nouns (e.g.

Syntactic Entrainment: The Repetition of Syntactic Structures in Event Descriptions.

Syntactic structures can convey certain (subtle) emergent properties of events. For example, the double-object dative ("the doctor is giving a patient pills") can convey the successful transfer of possession, whereas its syntactic alternative, the prepositional dative ("the doctor is giving pills to a patient"), conveys just a transfer to a location. Four experiments explore how syntactic structures may become associated with particular semantic content - such as these emergent properties of events.

Beyond linear order: The role of argument structure in speaking.

The current study examines how speakers plan sentences in which two words that form hierarchical dependency relationships - arguments and verbs - appear far apart in linear distance, to investigate how linear and hierarchical aspects of sentences simultaneously shape sentence planning processes. The results of six extended picture-word interference experiments suggest that speakers retrieve sentence-final verbs before the articulation of their sentence-initial patient or theme arguments, but not agent arguments, and before retrieving sentence-medial nouns inside modifiers. These results suggest that the time-course of sentence planning reflects hierarchically-defined dependency relationships over and above linear structure.

Zeb1 potentiates genome-wide gene transcription with Lef1 to promote glioblastoma cell invasion.

Glioblastoma is the most common and aggressive brain tumor, with a subpopulation of stem-like cells thought to mediate its recurring behavior and therapeutic resistance. The epithelial-mesenchymal transition (EMT) inducing factor Zeb1 was linked to tumor initiation, invasion, and resistance to therapy in glioblastoma, but how Zeb1 functions at molecular level and what genes it regulates remain poorly understood. Contrary to the common view that EMT factors act as transcriptional repressors, here we show that genome-wide binding of Zeb1 associates with both activation and repression of gene expression in glioblastoma stem-like cells.

Advances, challenges, and opportunities in extracellular RNA biology: insights from the NIH exRNA Strategic Workshop.

Extracellular RNA (exRNA) has emerged as an important transducer of intercellular communication. Advancing exRNA research promises to revolutionize biology and transform clinical practice. Recent efforts have led to cutting-edge research and expanded knowledge of this new paradigm in cell-to-cell crosstalk; however, gaps in our understanding of EV heterogeneity and exRNA diversity pose significant challenges for continued development of exRNA diagnostics and therapeutics.

Molecular hydrogen in the treatment of acute and chronic neurological conditions: mechanisms of protection and routes of administration.

Oxidative stress caused by reactive oxygen species is considered a major mediator of tissue and cell injuries in various neuronal conditions, including neurological emergencies and neurodegenerative diseases. Molecular hydrogen is well characterized as a scavenger of hydroxyl radicals and peroxynitrite. Recently, the neuroprotective effects of treatment with molecular hydrogen have been reported in both basic and clinical settings.

Obstacles and opportunities in the functional analysis of extracellular vesicle RNA - an ISEV position paper.

The release of RNA-containing extracellular vesicles (EV) into the extracellular milieu has been demonstrated in a multitude of different cell systems and in a variety of body fluids. RNA-containing EV are in the limelight for their capacity to communicate genetically encoded messages to other cells, their suitability as candidate biomarkers for diseases, and their use as therapeutic agents. Although EV-RNA has attracted enormous interest from basic researchers, clinicians, and industry, we currently have limited knowledge on which mechanisms drive and regulate RNA incorporation into EV and on how RNA-encoded messages affect signalling processes in EV-targeted cells.

Astrocytic Calcium Waves Signal Brain Injury to Neural Stem and Progenitor Cells.

Brain injuries, such as stroke or trauma, induce neural stem cells in the subventricular zone (SVZ) to a neurogenic response. Very little is known about the molecular cues that signal tissue damage, even over large distances, to the SVZ. Based on our analysis of gene expression patterns in the SVZ, 48 hr after an ischemic lesion caused by middle cerebral artery occlusion, we hypothesized that the presence of an injury might be transmitted by an astrocytic traveling calcium wave rather than by diffusible factors or hypoxia.

Linguistic representations and memory architectures: The devil is in the details.

Attempts to explain linguistic phenomena as consequences of memory constraints require detailed specification of linguistic representations and memory architectures alike. We discuss examples of supposed locality biases in language comprehension and production, and their link to memory constraints. Findings do not generally favor Christiansen & Chater's (C&C's) approach.

The timing of verb selection in Japanese sentence production.

Many influential models of sentence production (e.g., Bock & Levelt, 1994; Kempen & Hoenkamp, 1987; Levelt, 1989) emphasize the central role of verbs in structural encoding, and thus predict that verbs should be selected early in sentence formulation, possibly even before the phonological encoding of the first constituent (Ferreira, 2000).