Four years of monitoring antibiotic resistance in microorganisms from bacteremic patients.

From the second semester of 2002 to the end of the first semester of 2005, a total of 2544 bacterial strains were isolated from the blood stream of patients with clinical sepsis and bacteremia hospitalized in six University Hospitals in the Slovak Republic. Almost 30% of strains were coagulase-negative staphylococci (CONS), about 14% were Staphylococcus aureus and, of the Gram-negative bacteria, up to 9% were Klebsiella pneumoniae. All CONS, S.

[Microbiological findings in children with an indwelling central venous catheter].

Introduction: Besides their obvious advantages for the patient, central venous catheters (CVC) also carry the risk of possible infectious complications. The purpose of our investigation was to carry out a microbiological evaluation of a 5-year set of paediatric patients with indwelling CVCs.

Patients And Methods: In the group were 218 CVCs inserted to 165 children over a period of 5 years.

Monitoring of antibiotic resistance in bacterial isolates from bacteremic patients.

The aim of the study was to monitor the prevalence of pathogens and development of resistance in bacteria isolated from bacteremic patients. Five University Clinics and/or Regional Hospitals in the Slovak Republic participated in the study and a total of 421 isolates were collected in the second half of the year 2002. The most prevalent organisms were coagulase-negative staphylococci (CONS) (19%), Staphylococcus aureus (18.

Detection of virulence factors in alpha-haemolytic Escherichia coli strains isolated from various clinical materials.

In total, 201 alpha-haemolytic Escherichia coli isolates from various clinical materials (urine samples and vaginal and rectal swabs) were examined by PCR for the presence of genes for the virulence factors alpha-haemolysin (hly), cytotoxic necrotising factor type 1 (cnf1), P-fimbriae (pap), S/F1C-fimbriae (sfa/foc), aerobactin (aer) and afimbrial adhesin (afaI). Among vaginal isolates, 96% were positive for cnf1, compared with 80% of urine strains (p 0.02) and 63% of rectal strains (p 0.

Non-albicans Candida species isolated from plastic devices.

In this work we point out an occurrence of non-albicans Candida species isolated from catheters, cannulas and drains. We detected eight non-albicans Candida species in 49 examined samples: C. parapsilosis (n = 26), C.

Virulence-associated factors in Escherichia coli strains isolated from children with urinary tract infections.

One hundred and sixty-eight strains of Escherichia coli were isolated from cases of pyelonephritis (24) and lower urinary tract infections (UTI) (144) from hospitalised and outpatient children up to 2 years old. These strains were investigated for the expression of P fimbriae (PF), mannose-resistant and mannose-sensitive haemagglutination, cell-surface hydrophobicity, serum resistance and the production of alpha-haemolysin (AH), colicins and aerobactin. PF, AH, aerobactin production and serum resistance were significantly more frequent amongst strains expressing mannose-resistant haemagglutination.

[Sorbitol-negative E. coli 016 in a 10-year-old child diagnosed with hemolytic-uremic syndrome and in his asymptomatic father].

In Escherichia coli strains isolated from faeces and smears from the rectum in haemolytic-uraemic syndrome of a child and healthy relatives the authors assessed the biochemical activity, antigenic characteristic, sensitivity to twelve antimicrobial substances and factors of virulence. Sorbitol negative strains were isolated from the ill child and his father. The strains belonged into serogroup 016.

Enterohaemolysin production in Escherichia coli strains.

Enterohaemolysin production was found in 11 (20.3%) out of 54 Escherichia coli strains isolated from stools of infants with dyspepsia and in 3 (2.3%) out of 130 E.