Capacity of Abbott RealTi MTB RIF/INH to detect rifampicin- and isoniazid-resistant tuberculosis.

Abbott RealTi MTB RIF/INH Resistance (RT RIF/INH) is a new assay for the detection of resistance to rifampicin (RIF) and isoniazid (INH) in tuberculosis (TB) patients. To evaluate the capacity of RT RIF/INH to detect resistance-associated mutations in target genes. A total of 311 strains that had been pre-characterised using genotypic methods (GenoType MTBDR, Sanger sequencing) and phenotypic drug susceptibility testing were subjected to DNA extraction on Abbott 2000 and analysed using RT RIF/INH.

Automated detection of bacterial growth on 96-well plates for high-throughput drug susceptibility testing of Mycobacterium tuberculosis.

M. tuberculosis grows slowly and is challenging to work with experimentally compared with many other bacteria. Although microtitre plates have the potential to enable high-throughput phenotypic testing of M.

Evaluation of the Abbott RealTime MTB and RealTime MTB INH/RIF Assays for Direct Detection of Mycobacterium tuberculosis Complex and Resistance Markers in Respiratory and Extrapulmonary Specimens.

The Abbott RealTime MTB (RT MTB) assay is a new automated nucleic acid amplification test for the detection of Mycobacterium tuberculosis complex (MTBC) in clinical specimens. In combination with the RealTime MTB INH/RIF (RT MTB INH/RIF) resistance assay, which can be applied to RT MTB-positive specimens as an add-on assay, the tests also indicate the genetic markers of resistance to isoniazid (INH) and rifampin (RIF). We aimed to evaluate the diagnostic sensitivity and specificity of RT MTB using different types of respiratory and extrapulmonary specimens and to compare performance characteristics directly with those of the FluoroType MTB assay.

Development of laser induced fluorescence diagnostic for measuring the parameters of plasma containing rare gas species.

Laser induced fluorescence (LIF) technique development activity for measurement of plasma parameters in ITER divertor plasma is described. Helium density is the task of priority, but Doppler measurement of ion (atom) temperatures is also the aim of the program. The concept of ITER scenarios includes injection of "extrinsic" impurities (Ne, Ar, and Kr).

[Induction of T-suppressors of delayed-type hypersensitivity with a background of T-effector formation].

Administration to mice of 10(5) syngeneic splenocytes modified with trinitrobenzene sulfonic acid leads to the formation of a population of T suppressors which are capable to sorb on a specific antigen. In recipients, these cells suppress only one phase of the induction of delayed type hypersensitivity (DTH). Their precursors are sensitive to the action of low doses of cyclophosphamide.

[Isolation and some properties of N-acetyl-beta-D-hexosaminidase from Sarcoscipha coccinea].

N-Acetyl-beta-D-hexosaminidase was isolated from the extract of the discomycet Sarcoscipha coccinea and purified 510--550-fold by gel filtration on Sephadex G-200 and by ion-exchange chromatography on KM-Sephadex C-50 and DEAE-Sephadex A-50 or by a combination of hydrophobic and affinity chromatographies. Gel electrophoresis confirmed the homogeneity of the enzyme in both cases. Some properties of purified N-acetyl-beta-D-hexosaminidase (e.

[Isolation and properties of N-acetyl-beta-D-hexosaminidase from the fungus Hohenbuechelia serotina].

N-Acetyl-beta-D-hexosaminidase (EC 3.2.1.

[Effect of acid polysaccharides on the corticosteroid biosynthesis].

Repeated administration of carboxymethylchitin and chondroitinsulfate to rats in doses of 20 and 120 mg/kg/24 hours led to reduction (by 40-55%) of the rate of aldosterone and 18-oxycorticosterone biosynthesis by the rat adrenal glands in vitro. Carboxymethylcellulose, algin and hyaluronic acid displayed no inhibitory effect. In case of a single administration of carboxymethylchitin to rats (50 mg/kg) inhibition of the rate of aldosterone and 18-oxycorticosterone biosynthesis was seen 48 hours after the administration of the preparation and lasted four days.

[Change in aldosterone secretion by the adrenals and in its urinary excretion in rats under the action of acid polysaccharides].

Repeated administration of chondroitin sulfate to rats in doses of 20 and 120 mg/kg/24 hours led to reduction in the level of urinary aldosterone excretion in rats. Carboxymethylcellulose and alginic acid displayed no inhibitory effect. Urinary aldosterone excretion decreased in rats 24 hours after a single administration of chondroitin sulfate; this effect persisted for the following 3-4 days.