Publications by authors named "Molly H Nelson Holte"
Menopause is a natural physiological process in older women that is associated with reduced estrogen production and results in increased risk for obesity, diabetes, and osteoporosis. 17α-estradiol (17α-E2) treatment in males, but not females, reverses several metabolic conditions associated with advancing age, highlighting sexually dimorphic actions on age-related pathologies. In this study we sought to determine if 17α-E2 could prevent ovariectomy (OVX)-mediated detriments on adiposity and bone parameters in females.
View Article and Find Full Text PDFAim: Tieg1 is involved in multiple signalling pathways, human diseases, and is highly expressed in muscle where its functions are poorly understood.
Methods: We have utilized Tieg1 knockout (KO) mice to identify novel and important roles for this transcription factor in regulating muscle ultrastructure, metabolism and mitochondrial functions in the soleus and extensor digitorum longus (EDL) muscles. RNA sequencing, immunoblotting, transmission electron microscopy, MRI, NMR, histochemical and mitochondrial function assays were performed.
Article Synopsis
- Diabetic gastroparesis often involves a depletion of interstitial cells of Cajal (ICCs), while some diabetic patients experience accelerated gastric emptying (GE), especially when accompanied by obesity and high blood glucose levels.
- Research using mutant mice revealed that hyperglycemia led to increased proliferation of ICCs and faster GE, possibly due to enhanced signaling pathways involving MAPK1 and MAPK3.
- Various experimental methods, including breath tests and genetic manipulation, were used to analyze the effects of hyperglycemia on ICCs and their role in gastric motility.
We have previously demonstrated that TGFβ Inducible Early Gene-1 (TIEG1), also known as KLF10, plays important roles in mediating skeletal development and homeostasis in mice. TIEG1 has also been identified in clinical studies as one of a handful of genes whose altered expression levels or allelic variations are associated with decreased bone mass and osteoporosis in humans. Here, we provide evidence for the first time that TIEG1 is involved in regulating the canonical Wnt signaling pathway in bone through multiple mechanisms of action.
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- The regulation of chromatin structure in eukaryotic cells involves architectural factors like HMGB proteins, which help balance genome accessibility and compaction.
- HMO1 specifically binds to ribosomal RNA gene regions, aiding transcription and stabilizing chromatin even without histones.
- By using techniques like single molecule stretching and atomic force microscopy, researchers found that HMO1 compacts DNA quickly and forms stable loops over time, suggesting it helps maintain stable regions of chromatin without nucleosomes through dynamic DNA structures.
Despite progress in cardiovascular research, a cure for peripheral vascular disease has not been found. We compared the vascularization and tissue regeneration potential of murine and human undifferentiated multipotent adult progenitor cells (mMAPC-U and hMAPC-U), murine MAPC-derived vascular progenitors (mMAPC-VP), and unselected murine BM cells (mBMCs) in mice with moderate limb ischemia, reminiscent of intermittent claudication in human patients. mMAPC-U durably restored blood flow and muscle function and stimulated muscle regeneration, by direct and trophic contribution to vascular and skeletal muscle growth.
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