Will the white blood cells tell? A potential novel tool to assess broiler chicken welfare.

This study assessed qualitative and quantitative leukocyte evaluation as potential broiler chicken welfare indicators, contributing to the limited literature on white blood cell (WBC) morphology as a diagnostic tool for welfare. Broiler chicken welfare within four poultry houses (PH) 1 to 4, each on a different farm, was assessed using on-field indicators of affective states and health, and WBC morphology was examined. Affective states were evaluated using the Qualitative Behavior Assessment (QBA), with 25 behavioral expressions scored on a visual analogue scale (VAS) and grouped into two categories.

Work bout handling of a search dog reduces handgrip strength and increases firefighters' risk of falls.

Objectives: Firefighters work with dogs as support for their search activities for victims. Firefighters who handle dogs supposedly have higher acute muscle pain, more stumbling, and fatigue. This study aimed to verify the influence of a mantrailing dog on the firefighter's psychophysiological and muscular responses and the number of imbalances in a simulated activity of searching for people.

Welfare assessment of Thoroughbred horses naturally infected with gastrointestinal parasites in Southern Brazil: Quantifying the host-parasite relationship.

Horse welfare assessment (HWA) does not account for individual or herd parasite infection. This study investigated the connection between HWA and individual parasite fecal egg count (FEC) in 90 Thoroughbred horses. All horses were naturally infected with gastrointestinal parasites and were evaluated for individual welfare indicators and FEC monthly, for 12 months.

Animal Welfare Science: Why and for Whom?

There are, in the literature, distinct ways to approach animal welfare. The objective of this work was to study the value attributed to farm animals in the scientific papers published in animal welfare and animal production journals at three different points in time, separated by a decade each. The first ten papers mentioning "animal welfare" or "animal well-being" in their objectives or hypotheses from each journal and each focus year were selected.

Demand changes meat as changing meat reshapes demand: The great meat revolution.

As consumer acceptance and overall demand for the different types of meat are important determinants of the new balance between conventional and alternative meats, our goal was to approach the changes in meat demand, as affected by the increasingly available alternative meats coupled to the challenges of conventional meat including the meat paradox, with emphasis on the Brazilian scenario. Then, some aspects of the demand for alternative meats are presented, with a brief historical background. As the decisions taken in the present shape this unprecedented revolution in the way we produce and choose whether to eat meat and, if so, which one, the details of the transition to alternative meat chains in Brazil are yet to be written.

Ordinal or visual analogue scales for assessing aspects of broiler chicken welfare?

When the gradation of animal welfare is scored through ordinal scales and equidistant tags are used, empirical data between tags tend to be non-equidistant. Ordinal rate scales (ORS) and visual analogue scales (VAS) were tested for the assessment of contact dermatitis on breast and abdominal areas (CD), footpad dermatitis (FP), hock burns (HB) and bird soiling (BS) in broiler chickens. Calculations regarding the inter-rater reliability, the correlation between VAS and ORS and amongst the welfare indicators measured with both scales, and the equidistance of ORS categories in relation to values measured using VAS, were made.

Twelve Threats of Precision Livestock Farming (PLF) for Animal Welfare.

Research and development of Precision Livestock Farming (PLF) is booming, partly due to hopes and claims regarding the benefits of PLF for animal welfare. These claims remain largely unproven, however, as only few PLF technologies focusing on animal welfare have been commercialized and adopted in practice. The prevailing enthusiasm and optimism about PLF innovations may be clouding the perception of possible threats that PLF may pose to farm animal welfare.

From the Point of View of the Chickens: What Difference Does a Window Make?

We aimed to investigate what broiler chickens prefer when given free choice between a barn side with artificial lighting only as opposed to the other barn side with natural light through glass windows and artificial light. Eighty-five 1 day-old male Cobb 500 broiler chickens were divided into 10 pens; half of each pen area was provided with only artificial light (OAL) and the other half with natural and artificial light (NAL), and birds were free to move across sides. Environmental indicators and external conditions such as temperature, relative humidity, air velocity, ammonia and illuminance were monitored inside and outside the barn.

Mind the Gap: Animal Protection Law and Opinion of Sheep Farmers and Lay Citizens Regarding Animal Maltreatment in Sheep Farming in Southern Brazil.

We aimed to study the gaps between the law and sheep farmer and citizen opinions regarding animal maltreatment by discussing the risk of sheep maltreatment in regular farming practices in Southern Brazil. We surveyed the perception of 56 farmers and 209 citizens regarding general animal and specific on-farm sheep maltreatment issues. The main themes from these two groups about the key components of animal maltreatment were similar: failing to provide for the basic animal needs (27.

Welfare of broiler chickens reared in two different industrial house types during the winter season in Southern Brazil.

1. The following trial compared broiler chicken welfare in closed-sided (CS) open-sided (OS) industrial house types during the winter season in the South of Brazil.2.

Tilapia On-Farm Welfare Assessment Protocol for Semi-intensive Production Systems.

The aim of this study was to develop and test a tilapia on-farm welfare assessment protocol, based on Brazilian semi-intensive production systems. The study included two mains steps: the elaboration of tilapia welfare protocol and its on-field feasibility test. The protocol, including the potential indicators organized into health, environmental, nutritional, and behavioral categories, was tested on three farms.

Critical Perspective of Animal Production Specialists on Cell-Based Meat in Brazil: From Bottleneck to Best Scenarios.

Recently, many studies regarding consumer perception of cell-based meat have been published. However, the opinion of the professionals involved in animal production also seems relevant. In particular, veterinarians and animal scientists may be important players in the new cell-based meat production, acting as proponents or barriers to this major improvement for farm animal welfare.

Uncoupling Meat From Animal Slaughter and Its Impacts on Human-Animal Relationships.

Slaughter sets the debate about what is acceptable to do to animals at an extremely low bar. Recently, there has been considerable investment in developing cell-based meat, an alternative meat production process that does not require the raising and slaughtering of animals, instead using muscle cells cultivated in a bioreactor. We discuss the animal ethics impacts of cell-based and plant-based meat on human-animal interactions from animal welfare and rights perspectives, focusing on industrial meat production scenarios.

Animal Welfare Centres: Are They Useful for the Improvement of Animal Welfare?

Animal welfare has emerged as a scientific discipline only in the past 30 years, but a significant body of scientists has developed worldwide in this time. Over the past quarter century, several aggregations of scientists, centres of animal welfare, have become established. This can bring the benefits of the recognition of expertise and an opportunity to support animal industries in improving welfare, but it also brings the risk of scientists being influenced by these industries and failing to identify animal welfare problems as such.

Factors Related to Animal Neglect in Brazilian Laboratories.

Our objectives were to identify the prevalence of negligence of laboratory animals in Brazil, to determine the primary factors associated with its occurrence and to suggest prevention strategies. A questionnaire was made available online between October 2015 and March 2016. A total of 116 respondents with experience in the use of laboratory animals and/or the use of alternative methods answered the questionnaire.

Only When It Feels Good: Specific Cat Vocalizations Other Than Meowing.

Our objective was to identify and characterize the types of vocalization other than meowing (VOM) in two contexts, a pleasant and an aversive situation, and to study the effect of the sex of the animal. A total of 74 cats (32 tom cats and 42 queens) living in the city of Curitiba, Brazil, participated in the study; in total, 68 (29 tom cats and 39 queens) were divided into two groups according to the stimulus they were exposed to: either a pleasant situation (PS), when they were offered a snack, or an aversive situation (AS), with the simulation of a car transport event. The other six animals (three tom cats and three queens) participated in both situations.

First glimpse on attitudes of highly educated consumers towards cell-based meat and related issues in Brazil.

Our aim was to study Brazilian consumer attitudes towards cell-based meat and related issues. From 408 respondents from Curitiba and 218 from Joinville, the majority was women with higher level of education; 65.2% and 70.

Spatial distance and reactivity traits alter the positive perception of brushing by ewes.

Gentle handling seems to elicit positive states in sheep. The study investigated whether spatial distance alters sheep responses to brushing and whether spatial distance is influenced by reactivity. Twenty Romane ewes were assessed in three sessions: in Sessions 1 and 3, one grid separated the test animal from pen mates, with no distance between them, and in Session 2 two grids separated the test animal from pen mates by a distance of about 1.

Perception of animal sentience by Brazilian and French citizens: The case of sheep welfare and sentience.

The study compared the perception of ordinary citizens from Curitiba, Brazil (OB) and Clermont-Ferrand, France (OF), as well as OB, Brazilian veterinarians (VB), biologists (BB) and animal scientists (AB), concerning animal welfare and sentience. An online survey containing 18 open-ended, multiple choices and Likert scale questions was conducted from November 2014 to May 2016. The survey covered questions on demographics, perception of animal welfare, sheep welfare, sentience and animals' emotional capacities.

Perception of Fish Sentience, Welfare and Humane Slaughter by Highly Educated Citizens of Bogotá, Colombia and Curitiba, Brazil.

Discussions on farm animal welfare have become frequent, especially in developed countries. The aim of this research was to study the perception of fish sentience, welfare and slaughter by highly educated citizens from Bogotá, Colombia, and Curitiba, Brazil. An online survey with 12 questions presented as open-ended, multiple choice and 5-point Likert-type scale formats was available to respondents.

The use of a decision tree based on the rabies diagnosis scenario, to assist the implementation of alternatives to laboratory animals.

Brazilian federal legislation makes the use of alternatives mandatory, when there are validated methods to replace the use of laboratory animals. The objective of this paper is to introduce a novel decision tree (DT)-based approach, which can be used to assist the replacement of laboratory animal procedures in Brazil. This project is based on a previous analysis of the rabies diagnosis scenario, in which we identified certain barriers that hinder replacement, such as: a) the perceived higher costs of alternative methods; b) the availability of staff qualified in these methods; c) resistance to change by laboratory staff; d) regulatory obstacles, including incompatibilities between the Federal Environmental Crimes Act and specific norms and working practices relating to the use of laboratory animals; and e) the lack of government incentives.

Assessment of welfare of Brazilian and Belgian broiler flocks using the Welfare Quality protocol.

The Welfare Quality consortium has proposed a science-based protocol for assessing broiler chicken welfare on farms. Innovative features make the protocols particularly suited for comparative studies, such as the focus on animal-based welfare measures and an integration procedure for calculating an overall welfare status. These protocols reflect the scientific status up to 2009 but are meant to be updated on the basis of inter alia implementation studies.

Comparative costs of the Mouse Inoculation Test (MIT) and Virus Isolation in Cell Culture (VICC) for use in rabies diagnosis in Brazil.

The decision to use laboratory animals rather than in vitro methods is frequently based on the financial costs involved, so the objective of our study was to compare the costs of performing the Mouse Inoculation Test (MIT) and Virus Isolation in Cell Culture (VICC) for use in rabies diagnosis in Brazil. Based on observations of laboratory routines at the Pasteur Institute, São Paulo, we listed the fixed cost (FC) and variable cost (VC) items necessary to perform both tests. Considering that 200 MITs are equivalent to 350 VICC assays, in terms of facilities and staff-hours needed per month, we calculated, for both tests, the average total cost per sample, the costs of the implementation of the laboratory structure, and the costs of routine use.